Home>>Signaling Pathways>> DNA Damage/DNA Repair>> HDAC>>UF 010

UF 010

Catalog No.GC14367

Novel and selective class I HDAC inhibitor

Products are for research use only. Not for human use. We do not sell to patients.

UF 010 Chemical Structure

Cas No.: 537672-41-6

Size Price Stock Qty
10mM (in 1mL DMSO)
$45.00
In stock
10mg
$38.00
In stock
50mg
$149.00
In stock

Tel:(909) 407-4943 Email: sales@glpbio.com

Customer Reviews

Based on customer reviews.

  • GlpBio Citations

    GlpBio Citations
  • Bioactive Compounds Premium Provider

    Bioactive Compounds Premium Provider

Sample solution is provided at 25 µL, 10mM.

Product Documents

Quality Control & SDS

View current batch:

Background

UF 010 is a novel and selective class I HDAC inhibitor with IC50 values of 0.5, 0.1, 0.06, 1.5, 9.1, 15.3 and 44.5 μM for HDAC1, 2, 3, 8, 6, 10 and 11 [1].

Histone deacetylases (HDACs) are a class of enzymes that remove acetyl groups from ε-N-acetyl lysines on histones, allowing the histones to wrap the DNA more tightly. DNA expression is regulated by de-acetylation and acetylation.

UF 010 is a novel and selective class I HDAC inhibitor, and is a fast-on but slow-off inhibitor. In fluorescence assays, UF 010 (5 nM to 100 μM) exhibited inhibitory potency with IC50 values of 0.5, 0.1, 0.06, 1.5, 9.1, 15.3 and 44.5 μM for HDAC1, 2, 3, 8, 6, 10 and 11, and exhibited IC50 values of >100 μM for HDAC4, 5, 7 and 9. In HCT116 cells, UF 010 induced the accumulation of acetylated histones at all sites examined and significantly inhibited cellular HDACs. In HCT116 cell cultures, UF 010 inhibited HDAC with IC50 value of 1.8 μM. UF 010 also exhibited IC50 value of 11.2 μM for killing cells. In HepG2 liver cancer cell line, UF 010 inhibited cell survival with IC50 value of 4.6 μM. In MDA-MB-231 cells, UF 010 induced G1/S arrest with increased cells in G1 phase and reduced cells in S phase in a dose-dependent way. UF010 at 1 μM also significantly slowed migration [1].

Reference:
[1].  Wang Y, Stowe RL, Pinello CE, et al. Identification of histone deacetylase inhibitors with benzoylhydrazide scaffold that selectively inhibit class I histone deacetylases. Chem Biol, 2015, 22(2): 273-284.

Chemical Properties

Cas No. 537672-41-6 SDF
Chemical Name 4-bromo-N'-butylbenzohydrazide
Canonical SMILES O=C(NNCCCC)C1=CC=C(Br)C=C1
Formula C11H15BrN2O M.Wt 271.15
Solubility ≥ 10.65mg/mL in DMSO Storage Store at -20°C
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table

Prepare stock solution
1 mg 5 mg 10 mg
1 mM 3.688 mL 18.44 mL 36.88 mL
5 mM 0.7376 mL 3.688 mL 7.376 mL
10 mM 0.3688 mL 1.844 mL 3.688 mL
  • Molarity Calculator

  • Dilution Calculator

Mass
=
Concentration
x
Volume
x
MW*
 
 
 
**When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / CoA (available online).

Calculate

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.

Reviews

Review for UF 010

Average Rating: 5 ★★★★★ (Based on Reviews and 29 reference(s) in Google Scholar.)

5 Star
100%
4 Star
0%
3 Star
0%
2 Star
0%
1 Star
0%
Review for UF 010

GLPBIO products are for RESEARCH USE ONLY. Please make sure your review or question is research based.

Required fields are marked with *

You may receive emails regarding this submission. Any emails will include the ability to opt-out of future communications.