Dihydrokaempferol (Synonyms: (+)-Aromadendrin, (+)-Dihydrokaempferol, Dihydrokaempferol, trans-Dihydrokaempferol) |
Catalog No.GC38617
One-Click Copy Product Info
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Dihydrokaempferol (Aromadendrin) is a naturally occurring flavonoid compound with biological activities such as free radical scavenging, anti-inflammatory and anti-tumor.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 480-20-6
Sample solution is provided at 25 µL, 10mM.
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Dihydrokaempferol (Aromadendrin) is a naturally occurring flavonoid compound with biological activities such as free radical scavenging, anti-inflammatory and anti-tumor[1, 2]. Dihydrokaempferol can activate the SIRT1 pathway, thereby activating cell autophagy, reducing oxidative stress damage and inhibiting inflammatory response[3].
In vitro, Dihydrokaempferol (10, 30μM) treatment of rheumatoid arthritis fibroblast-like synoviocytes (RA-FLSs) for 48h concentration-dependently inhibited cell viability, induced cell apoptosis and was able to express apoptosis-related proteins[4]. Dihydrokaempferol (0-200μM) pretreatment of RAW264.7 macrophages for 1h inhibited LPS-induced NO and PGE2 production in a concentration-dependent manner[5]. Dihydrokaempferol (20μM) pretreatment of SH-SY5y neuronal cells for 1h inhibited methamphetamine (METH)-induced cytotoxicity and enhanced cell confluence[6].
In vivo, oral administration of Dihydrokaempferol (80mg/kg) to treat severe acute pancreatitis (SAP) mice regulated the Keap1/Nrf2 pathway, reduced oxidative stress damage, and improved SAP-induced pancreatic damage[7].
References:
[1] Kim J K, Park S U. Recent studies on kaempferol and its biological and pharmacological activities[J]. EXCLI journal, 2020, 19: 627-634.
[2] Wang S H, Hu Y L, Liu T X. Plant distribution and pharmacological activity of flavonoids[J]. Tradit. Med. Res, 2019, 4: 269-287.
[3] Zhang J, Hu C, Li X, et al. Protective effect of dihydrokaempferol on acetaminophen-induced liver injury by activating the SIRT1 pathway[J]. The American Journal of Chinese Medicine, 2021, 49(03): 705-718.
[4] Zhang Y, Yan G, Sun C, et al. Apoptosis effects of dihydrokaempferol isolated from Bauhinia championii on synoviocytes[J]. Evidence‐Based Complementary and Alternative Medicine, 2018, 2018(1): 9806160.
[5] Lee J W, Kim N H, Kim J Y, et al. Aromadendrin inhibits lipopolysaccharide-induced nuclear translocation of NF-κB and phosphorylation of JNK in RAW 264.7 macrophage cells[J]. Biomolecules & Therapeutics, 2013, 21(3): 216.
[6] Lee H S, Kim E N, Jeong G S. Aromadendrin protects neuronal cells from methamphetamine-induced neurotoxicity by regulating endoplasmic reticulum stress and PI3K/Akt/mTOR signaling pathway[J]. International Journal of Molecular Sciences, 2021, 22(5): 2274.
[7] Liang X, Hu C, Liu C, et al. Dihydrokaempferol (DHK) ameliorates severe acute pancreatitis (SAP) via Keap1/Nrf2 pathway[J]. Life Sciences, 2020, 261: 118340.
| Cell experiment [1]: | |
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Cell lines |
Rheumatoid arthritis fibroblast-like synoviocytes (RA-FLSs) |
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Preparation Method |
RA-FLSs cultured in 96-well plates were treated with Dihydrokaempferol at various concentrations (0.3, 3, 30, 300μM) for 48h, followed by incubation with MTS for an additional 4h at 37°C. Then the absorbance at 570nm was taken by a microplate reader. |
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Reaction Conditions |
0.3, 3, 30, 300μM; 48h |
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Applications |
Dihydrokaempferol (0.3, 3, 30, 300μM) had no significant effect of cell survival on normal synoviocytes. But Dihydrokaempferol (0.3, 3, 30, 300μM) concentration dependently decreased the viability of RA-FLSs. |
| Animal experiment [2]: | |
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Animal models |
C57BL/6 mice |
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Preparation Method |
Mice were randomly divided into six distinct groups as follows: ad-control, ad-control+Severe acute pancreatitis (SAP), ad-control+SAP+Dihydrokaempferol (80mg/kg), ad-Keap1, ad- Keap1+SAP, and ad-Keap1+SAP+Dihydrokaempferol (80mg/kg). These mice received seven i.p. of 50μg/kg cerulein diluted in saline, with a 1 h interval between the injections. On the last injection, these mice received an injection that contained both cerulein and LPS (10mg/kg). Subsequently, these mice were intravenously (i.v.) injected with ad-Keap1 (5×108 pfu) or ad-control (5×108 pfu) to be infected and Dihydrokaempferol administered orally 1h after SAP induction. Lastly, these mice were sacrificed 6h after SAP induction and their plasma and pancreas tissues collected. |
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Dosage form |
80mg/kg; p.o. |
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Applications |
Keap1 overexpression adenovirus (ad-Keap1) suppressed the nuclear translocation of Nrf2 which is enhanced by Dihydrokaempferol, and suppressed the antioxidant functionality of Dihydrokaempferol both in mice and cells models. |
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References: |
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| Cas No. | 480-20-6 | SDF | |
| Synonyms | (+)-Aromadendrin, (+)-Dihydrokaempferol, Dihydrokaempferol, trans-Dihydrokaempferol | ||
| Canonical SMILES | O=C1C2=C(O)C=C(O)C=C2O[C@H](C3=CC=C(O)C=C3)[C@H]1O | ||
| Formula | C15H12O6 | M.Wt | 288.25 |
| Solubility | DMSO: 10 mM | Storage | 4°C, protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.4692 mL | 17.3461 mL | 34.6921 mL |
| 5 mM | 693.8 μL | 3.4692 mL | 6.9384 mL |
| 10 mM | 346.9 μL | 1.7346 mL | 3.4692 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.50% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 15 reference(s) in Google Scholar.)