| BODIPY 493/503 Catalog No.GC42959 |
Sample solution is provided at 25 µL, 10mM.
Quality Control & SDS
- View current batch:
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Purity = 99.50%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
| Cell experiment : | |
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Preparation method |
The stock solution of this compound can be prepared by dissolving 1.3 mg of the product in 1ml DMSO to a concentration of 5 mM. The staining solution can be prepared by diluting stock solution in PBS. 2 μM BODIPY staining solution can be prepared by diluting stock solution 1:2,500 in PBS. |
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Reaction Conditions |
Incubate in the dark for 15 min at 37 °C |
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Applications |
This compound can be used as a stain for neutral lipds and as a tracer for oil and other nonpolar lipids. |
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References: [1]. Qiu, M Celeste, & Simon. (2016). Bodipy 493/503 staining of neutral lipid droplets for microscopy and quantification by flow cytometry. Bio-protocol. [2]. Wen-Chung, H. , Ya-Ling, C. , Hui-Chia, L. , Shu-Ju, W. , & Chian-Jiun, L. . (2018). Ginkgolide c reduced oleic acid-induced lipid accumulation in hepg2 cells. Saudi Pharmaceutical Journal, 26, S1319016418301452-. [3]. Karmann, S. , Follonier, Stéphanie, Bassas-Galia, M. , Panke, S. , & Zinn, M. . (2016). Robust at-line quantification of poly(3-hydroxyalkanoate) biosynthesis by flow cytometry using a bodipy 493/503-syto 62 double-staining. Journal of Microbiological Methods, 166-171. |
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| Cas No. | 121207-31-6 | SDF | Download SDF |
| Synonyms | N/A | ||
| Chemical Name | (T-4)-[2-[1-(3,5-dimethyl-2H-pyrrol-2-ylidene-κN)ethyl]-3,5-dimethyl-1H-pyrrolato-κN]difluoro-boron | ||
| Canonical SMILES | [F-][B+3]([N-]1C2=C(C)C=C1C)([N](C(C(C)=C3)=C2C)=C3C)[F-] | ||
| Formula | C14H17BF2N2 | M.Wt | 262.1 |
| Solubility | Soluble in DMSO, DMF, methanol, chloroform, | Storage | Store at -20°C |
| General tips | For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months. | ||
| Shipping Condition | Evaluation sample solution : ship with blue ice All other available size: ship with RT , or blue ice upon request |
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BODIPY 493/503 is a lipophilic fluorescent probe that localizes to polar lipids and can be used to label cellular neutral lipid contents, particularly those localized to lipid droplets, in live and fixed cells.[1],[2] BODIPY 493/503 is compatible with epifluorescent, confocal, and two-photon microscopy, as well as flow cytometry. It displays excitation/emission maxima of 493/503 nm, respectively, and can be used for live and fixed cell applications.
Reference:
[1]. Listenberger, L.L., and Brown, D.G. Fluorescent Detection of Lipid Droplets and Associated Proteins. Curr. Protoc. Cell. Biol. Supplement 35, 24.22.21-24.22.11 (2007).
[2]. Qiu, B., and Simon, M.C. BODIPY 493/503 staining of neutral lipid droplets for microscopy and quantification by flow cytometry. Bio. Protoc. 6(17), (2016).