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BODIPY 493/503 Catalog No.GC42959

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Sample solution is provided at 25 µL, 10mM.

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Preparation method

The stock solution of this compound can be prepared by dissolving 1.3 mg of the product in 1ml DMSO to a concentration of 5 mM. The staining solution can be prepared by diluting stock solution in PBS. 2 μM BODIPY staining solution can be prepared by diluting stock solution 1:2,500 in PBS.

Reaction Conditions

Incubate in the dark for 15 min at 37 °C


This compound can be used as a stain for neutral lipds and as a tracer for oil and other nonpolar lipids.


[1]. Qiu, M Celeste, & Simon. (2016). Bodipy 493/503 staining of neutral lipid droplets for microscopy and quantification by flow cytometry. Bio-protocol.

[2]. Wen-Chung, H. , Ya-Ling, C. , Hui-Chia, L. , Shu-Ju, W. , & Chian-Jiun, L. . (2018). Ginkgolide c reduced oleic acid-induced lipid accumulation in hepg2 cells. Saudi Pharmaceutical Journal, 26, S1319016418301452-.

[3]. Karmann, S. , Follonier, Stéphanie, Bassas-Galia, M. , Panke, S. , & Zinn, M. . (2016). Robust at-line quantification of poly(3-hydroxyalkanoate) biosynthesis by flow cytometry using a bodipy 493/503-syto 62 double-staining. Journal of Microbiological Methods, 166-171.

Chemical Properties

Cas No. 121207-31-6 SDF Download SDF
Synonyms N/A
Chemical Name (T-4)-[2-[1-(3,5-dimethyl-2H-pyrrol-2-ylidene-κN)ethyl]-3,5-dimethyl-1H-pyrrolato-κN]difluoro-boron
Canonical SMILES [F-][B+3]([N-]1C2=C(C)C=C1C)([N](C(C(C)=C3)=C2C)=C3C)[F-]
Formula C14H17BF2N2 M.Wt 262.1
Solubility Soluble in DMSO, DMF, methanol, chloroform, Storage Store at -20°C
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.
Shipping Condition Evaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request
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**When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / CoA (available online).



BODIPY 493/503 is a lipophilic fluorescent probe that localizes to polar lipids and can be used to label cellular neutral lipid contents, particularly those localized to lipid droplets, in live and fixed cells.[1],[2] BODIPY 493/503 is compatible with epifluorescent, confocal, and two-photon microscopy, as well as flow cytometry. It displays excitation/emission maxima of 493/503 nm, respectively, and can be used for live and fixed cell applications.

[1]. Listenberger, L.L., and Brown, D.G. Fluorescent Detection of Lipid Droplets and Associated Proteins. Curr. Protoc. Cell. Biol. Supplement 35, 24.22.21-24.22.11 (2007).
[2]. Qiu, B., and Simon, M.C. BODIPY 493/503 staining of neutral lipid droplets for microscopy and quantification by flow cytometry. Bio. Protoc. 6(17), (2016).