|BODIPY 493/503 Catalog No.GC42959|
Sample solution is provided at 25 µL, 10mM.
|Cell experiment :|
The stock solution of this compound can be prepared by dissolving 1.3 mg of the product in 1ml DMSO to a concentration of 5 mM. The staining solution can be prepared by diluting stock solution in PBS. 2 μM BODIPY staining solution can be prepared by diluting stock solution 1:2,500 in PBS.
Incubate in the dark for 15 min at 37 °C
This compound can be used as a stain for neutral lipds and as a tracer for oil and other nonpolar lipids.
. Qiu, M Celeste, & Simon. (2016). Bodipy 493/503 staining of neutral lipid droplets for microscopy and quantification by flow cytometry. Bio-protocol.
. Wen-Chung, H. , Ya-Ling, C. , Hui-Chia, L. , Shu-Ju, W. , & Chian-Jiun, L. . (2018). Ginkgolide c reduced oleic acid-induced lipid accumulation in hepg2 cells. Saudi Pharmaceutical Journal, 26, S1319016418301452-.
. Karmann, S. , Follonier, Stéphanie, Bassas-Galia, M. , Panke, S. , & Zinn, M. . (2016). Robust at-line quantification of poly(3-hydroxyalkanoate) biosynthesis by flow cytometry using a bodipy 493/503-syto 62 double-staining. Journal of Microbiological Methods, 166-171.
|Cas No.||121207-31-6||SDF||Download SDF|
|Solubility||Soluble in DMSO, DMF, methanol, chloroform,||Storage||Store at -20°C|
|General tips||For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.|
|Shipping Condition||Evaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request
BODIPY 493/503 is a lipophilic fluorescent probe that localizes to polar lipids and can be used to label cellular neutral lipid contents, particularly those localized to lipid droplets, in live and fixed cells., BODIPY 493/503 is compatible with epifluorescent, confocal, and two-photon microscopy, as well as flow cytometry. It displays excitation/emission maxima of 493/503 nm, respectively, and can be used for live and fixed cell applications.
. Listenberger, L.L., and Brown, D.G. Fluorescent Detection of Lipid Droplets and Associated Proteins. Curr. Protoc. Cell. Biol. Supplement 35, 24.22.21-24.22.11 (2007).
. Qiu, B., and Simon, M.C. BODIPY 493/503 staining of neutral lipid droplets for microscopy and quantification by flow cytometry. Bio. Protoc. 6(17), (2016).