Azoxymethane |
| Catalog No.GC19445 |
Azoxymethane, as a colon carcinogen, can lead to the formation of DNA adducts[1].
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 25843-45-2
Sample solution is provided at 25 µL, 10mM.
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Azoxymethane, as a colon carcinogen, can lead to the formation of DNA adducts[1]. Azoxymethane (AOM) causes O(6)-methylguanine adduct formation which leads to G-->A transitions[2].
In vivo, 7-week-old conventional male C57BL/6 mice were injected intraperitoneally with 6 doses of 10 mg/kg AOM, feces from obese individuals promote colorectal cancer formation in AOM-treated CRC mouse model[3]. SWR/J, A/J mice was treated with 10 mg/kg AOM i.p. once a week for 8 weeks developed tumors, with a distribution that was limited to the distal colon[4]. In wild-type (WT) mice, AOM treatment (10 mg/kg) induced a 2.5-fold increase in intestinal crypt stem cell survival. WT mice receiving AOM exhibited increased intestinal apoptosis and a simultaneous reduction in crypt mitotic figures within 8 h of injection[5].
[1] Chen W, et al. Effect of tea on the formation of DNA adducts by azoxymethane. Xenobiotica. 1998 Feb;28(2):213-7.
[2] Wali RK, et al. Inhibition of O(6)-methylguanine-DNA methyltransferase increases azoxymethane-induced colonic tumors in rats. Carcinogenesis. 1999 Dec;20(12):2355-60.
[3] Kang X, et al. Altered gut microbiota of obesity subjects promotes colorectal carcinogenesis in mice. EBioMedicine. 2023 Jul;93:104670.
[4] Papanikolaou A, et al. Azoxymethane-induced colon tumors and aberrant crypt foci in mice of different genetic susceptibility. Cancer Lett. 1998 Aug 14;130(1-2):29-34.
[5] Riehl TE, et al. Azoxymethane protects intestinal stem cells and reduces crypt epithelial mitosis through a COX-1-dependent mechanism. Am J Physiol Gastrointest Liver Physiol. 2006 Dec;291(6):G1062-70.
References:
| Cell experiment [1]: | |
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Cell lines |
hepatic microsomes |
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Preparation Method |
The assay for AOM-induced in vitro DNA adduct formation was based on a published method. Briefly, microsomes (0.5-2.0 mg/mL) were incubated with calf thymus DNA (1 mg/mL) and AOM (200 µM) in a total volume of 1.0 mL. The assay buffer consisted of 0.1 M Tris-HCl (pH 7.4), 1 mM EDTA, 20 mM MgCl2, 0.3 M KCl, and 1.5 mM NADPH. Incubations were carried out at 37 °C for 60 min in a shaking water bath. An additional 30 nmol of NADPH was added after the first 30 min. The reaction was stopped by the addition of 0.5 mL of ice-cold 7.5 M ammonium acetate. DNA was then extracted as described above for tissue homogenates. Control incubations were performed without NADPH. |
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Reaction Conditions |
200 µM; 60 min |
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Applications |
On an equal protein basis, hepatic microsomes were much more active than SI and colon microsomes in NADPH-dependent AOM bioactivation and N7-mG adduct formation. Microsomes of the liver (but not those of the SI or colon) of LCN mice showed significantly lower activity in N7-mG formation compared to that of WT mice. |
| Animal experiment [2]: | |
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Animal models |
8 to 10-week-old male C57BL/6J mice |
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Preparation Method |
On day 0, mark and weight each mice. Prepare 1 mg/mL (1 µg/µL) AOM working solution in PBS, and inject each mouse intraperitoneally with 10 mg/kg (body weight) of AOM (For example, a 20 g mouse shall be injected with 200 µL AOM solution). Then each mice was providedwith 2.5% DSS-containing drinking water for 7 days, followed with normal water for 10 days. This cycle of DSS -containing water and normal water shall be repeated three times, and then the normal water treatment stays last to the experimental endpoint. |
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Dosage form |
10 mg/kg, i.p. |
|
Applications |
The colitis-associated CRC (colorectal cancer) model in mice can be made by using AOM and DSS. AOM- and DSS-induced murine colorectal cancer model has a body weight dynamics. DSS administration could cause significant weight loss. |
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References: [1] Megaraj V, et al. Role of hepatic and intestinal p450 enzymes in the metabolic activation of the colon carcinogen azoxymethane in mice. Chem Res Toxicol. 2014 Apr 21;27(4):656-62. |
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| Cas No. | 25843-45-2 | SDF | |
| Canonical SMILES | C/[N+]([O-])=N/C | ||
| Formula | C2H6N2O | M.Wt | 74.08 |
| Solubility | Soluble in water, ethanol, and ether | Storage | Store at -20°C, protect from light; It is easy to evaporate, please use immediately after opening. |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 13.4989 mL | 67.4946 mL | 134.9892 mL |
| 5 mM | 2.6998 mL | 13.4989 mL | 26.9978 mL |
| 10 mM | 1.3499 mL | 6.7495 mL | 13.4989 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
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Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 12 reference(s) in Google Scholar.)
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