Blasticidin S HCl |
| Catalog No.: GC18094 |
Blasticidin S HCl is a nucleoside antibiotic isolated from Streptomyces griseochromogenes[1].
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.:3513-03-9
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
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Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
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Cell experiment [1]: |
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Cell lines |
HeLa cells |
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Preparation method |
Cells were seeded in six-well-plates and transfected after 24 h with 0.6 μg/well of the reporter plasmids pCI-Renilla-HBB WT or pCI-Renilla-HBB-Nonsense-Mutation 39 (NS39).After 16 h, the cells were washed with full medium and treated for 3 h with Blasticidin S HCl or untreated. |
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Reaction Conditions |
0-100μg/ml; 3 h |
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Applications |
Blasticidin S HCl treatment stabilizes the premature stop codon-mutated mRNA in HeLa cells. |
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References: [1]. Powers KT, Stevenson-Jones F,et,al. Blasticidin S inhibits mammalian translation and enhances production of protein encoded by nonsense mRNA. Nucleic Acids Res. 2021 Jul 21;49(13):7665-7679. doi: 10.1093/nar/gkab532. PMID: 34157102; PMCID: PMC8287960. |
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Blasticidin S HCl is a nucleoside antibiotic isolated from Streptomyces griseochromogenes[1]. Blasticidin S HCl specifically inhibits protein synthesis in prokaryotic or eukaryotic cells by interfering with ribosome binding peptides. The inhibitory effect of Blasticidin S HCl on translation termination is conserved in eukaryotes and bacteria[2-4].
Blasticidin S HCl treatment stabilizes the premature stop codon-mutated mRNA in HeLa cells, Blasticidin S HCl inhibits nonsense-mediated mRNA decay and, at subinhibitory concentrations, modulates translation dynamics at premature termination codons leading to enhanced protein production[5]. The presence of Blasticidin S HCl protects DNA from Cu(II) -induced damage[6].
References:
[1]. TAKEUCHI S, HIRAYAMA K, et,al. Blasticidin S, a new antibiotic. J Antibiot (Tokyo). 1958 Jan;11(1):1-5. PMID: 13525246.
[2]. Svidritskiy E, Korostelev AA. Mechanism of Inhibition of Translation Termination by Blasticidin S. J Mol Biol. 2018 Mar 2;430(5):591-593. doi: 10.1016/j.jmb.2018.01.007. PMID: 29366636; PMCID: PMC5831496.
[3]. Kinoshita T, Tanaka N, et,al. Binding of blasticidin S to ribosomes. J Antibiot (Tokyo). 1970 Jun;23(6):288-90. doi: 10.7164/antibiotics.23.288. PMID: 4917792.
[4]. Svidritskiy E, Ling C, et,al. Blasticidin S inhibits translation by trapping deformed tRNA on the ribosome. Proc Natl Acad Sci U S A. 2013 Jul 23;110(30):12283-8. doi: 10.1073/pnas.1304922110. Epub 2013 Jul 3. PMID: 23824292; PMCID: PMC3725078.
[5]. Powers KT, Stevenson-Jones F, et,al. Blasticidin S inhibits mammalian translation and enhances production of protein encoded by nonsense mRNA. Nucleic Acids Res. 2021 Jul 21;49(13):7665-7679. doi: 10.1093/nar/gkab532. PMID: 34157102; PMCID: PMC8287960.
[6]. Stokowa-Sołtys K, Jeżowska-Bojczuk M. A rice fungicide blasticidin S efficiently binds Cu(II) ions and prevents DNA from metal-induced damage. J Inorg Biochem. 2013 Oct;127:73-8. doi: 10.1016/j.jinorgbio.2013.06.014. Epub 2013 Jun 29. PMID: 23880034.
| Cas No. | 3513-03-9 | SDF | |
| Chemical Name | (2S,3S,6R)-3-[[(3R)-3-amino-5-[carbamimidoyl(methyl)amino]pentanoyl]amino]-6-(4-amino-2-oxopyrimidin-1-yl)-3,6-dihydro-2H-pyran-2-carboxylic acid;hydrochloride | ||
| Canonical SMILES | CN(CCC(CC(=O)NC1C=CC(OC1C(=O)O)N2C=CC(=NC2=O)N)N)C(=N)N.Cl | ||
| Formula | C17H27ClN8O5 | M.Wt | 458.9 |
| Solubility | Water: 92 mg/mL | Storage | Store at -20°C |
| General tips | For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months. | ||
| Shipping Condition | Evaluation sample solution : ship with blue ice All other available size: ship with RT , or blue ice upon request |
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Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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μL
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Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
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