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Blasticidin S HCl

Catalog No.: GC18094

Blasticidin S HCl is a nucleoside antibiotic isolated from Streptomyces griseochromogenes[1].

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Blasticidin S HCl Chemical Structure

Cas No.:3513-03-9

Size Price Stock Qty
10mg
$66.00
In stock
25mg
$147.00
In stock
50mg
$245.00
In stock
100mg
$325.00
In stock

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Sample solution is provided at 25 µL, 10mM.

Product Documents

Quality Control & SDS

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Protocol

Cell experiment [1]:

Cell lines

HeLa cells

Preparation method

Cells were seeded in six-well-plates and transfected after 24 h with 0.6 μg/well of the reporter plasmids pCI-Renilla-HBB WT or pCI-Renilla-HBB-Nonsense-Mutation 39 (NS39).After 16 h, the cells were washed with full medium and treated for 3 h with Blasticidin S HCl or untreated.

Reaction Conditions

0-100μg/ml; 3 h

Applications

Blasticidin S HCl treatment stabilizes the premature stop codon-mutated mRNA in HeLa cells.

References:

[1]. Powers KT, Stevenson-Jones F,et,al. Blasticidin S inhibits mammalian translation and enhances production of protein encoded by nonsense mRNA. Nucleic Acids Res. 2021 Jul 21;49(13):7665-7679. doi: 10.1093/nar/gkab532. PMID: 34157102; PMCID: PMC8287960.

Background

Blasticidin S HCl is a nucleoside antibiotic isolated from Streptomyces griseochromogenes[1]. Blasticidin S HCl specifically inhibits protein synthesis in prokaryotic or eukaryotic cells by interfering with ribosome binding peptides. The inhibitory effect of Blasticidin S HCl on translation termination is conserved in eukaryotes and bacteria[2-4].

Blasticidin S HCl treatment stabilizes the premature stop codon-mutated mRNA in HeLa cells, Blasticidin S HCl inhibits nonsense-mediated mRNA decay and, at subinhibitory concentrations, modulates translation dynamics at premature termination codons leading to enhanced protein production[5]. The presence of Blasticidin S HCl protects DNA from Cu(II) -induced damage[6].

References:

[1]. TAKEUCHI S, HIRAYAMA K, et,al. Blasticidin S, a new antibiotic. J Antibiot (Tokyo). 1958 Jan;11(1):1-5. PMID: 13525246.

[2]. Svidritskiy E, Korostelev AA. Mechanism of Inhibition of Translation Termination by Blasticidin S. J Mol Biol. 2018 Mar 2;430(5):591-593. doi: 10.1016/j.jmb.2018.01.007. PMID: 29366636; PMCID: PMC5831496.

[3]. Kinoshita T, Tanaka N, et,al. Binding of blasticidin S to ribosomes. J Antibiot (Tokyo). 1970 Jun;23(6):288-90. doi: 10.7164/antibiotics.23.288. PMID: 4917792.

[4]. Svidritskiy E, Ling C, et,al. Blasticidin S inhibits translation by trapping deformed tRNA on the ribosome. Proc Natl Acad Sci U S A. 2013 Jul 23;110(30):12283-8. doi: 10.1073/pnas.1304922110. Epub 2013 Jul 3. PMID: 23824292; PMCID: PMC3725078.

[5]. Powers KT, Stevenson-Jones F, et,al. Blasticidin S inhibits mammalian translation and enhances production of protein encoded by nonsense mRNA. Nucleic Acids Res. 2021 Jul 21;49(13):7665-7679. doi: 10.1093/nar/gkab532. PMID: 34157102; PMCID: PMC8287960.

[6]. Stokowa-Sołtys K, Jeżowska-Bojczuk M. A rice fungicide blasticidin S efficiently binds Cu(II) ions and prevents DNA from metal-induced damage. J Inorg Biochem. 2013 Oct;127:73-8. doi: 10.1016/j.jinorgbio.2013.06.014. Epub 2013 Jun 29. PMID: 23880034.

Chemical Properties

Cas No. 3513-03-9 SDF
Chemical Name (2S,3S,6R)-3-[[(3R)-3-amino-5-[carbamimidoyl(methyl)amino]pentanoyl]amino]-6-(4-amino-2-oxopyrimidin-1-yl)-3,6-dihydro-2H-pyran-2-carboxylic acid;hydrochloride
Canonical SMILES CN(CCC(CC(=O)NC1C=CC(OC1C(=O)O)N2C=CC(=NC2=O)N)N)C(=N)N.Cl
Formula C17H27ClN8O5 M.Wt 458.9
Solubility Water: 92 mg/mL Storage Store at -20°C
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.
Shipping Condition Evaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request

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