Coelenterazine 400a |
| Catalog No.GC43290 |
Coelenterazine 400a (Bisdeoxycoelenterazine), a derivative of Coelenterazine, is a Renilla luciferase (RLuc) substrate.
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Cas No.: 70217-82-2
Sample solution is provided at 25 µL, 10mM.
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Coelenterazine 400a is a bisdeoxy derivative of coelenterazine that displays an emission maximum of 395 nm following conversion by Renilla luciferase (Rluc). It is used in bioluminescence resonance energy transfer 2 (BRET2) protocols, whereas coelenterazine h , which displays an emission maximum of 475 nm upon conversion by Rluc, is used in BRET1 protocols. Coelenterazine 400a is commonly paired with class 1 and 3 GFP acceptors, including GFP2 and GFP10. BRET2 assays are commonly used in evaluating protein-protein interactions, including those involved in G protein-coupled receptor signaling.
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