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Diphenylterazine (DTZ)

Catalog No.GC33428

Diphenylterazine (DTZ) Chemical Structure

Size Price Stock Qty
5mg
$321.00
In stock
10mg
$506.00
In stock
50mg
$1,057.00
In stock

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Sample solution is provided at 25 µL, 10mM.

Quality Control

Quality Control & SDS

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Protocol

Cell experiment:

BALB/c mice are used and transfected with cells expressing teLuc, Antares, Antares2 and FLuc by injecting cells into the tail vein of BALB/c mice. After the diminishing of the FLuc bioluminescence, 0.3 µmol Diphenylterazine or furimazine is intraperitoneally injected. Mice are imaged with a 1-min exposure per frame over a course of 20 min. The images are processed using the Fiji image analysis software and the frames with highest signals in individual experiments are used for comparison[1].

References:

[1]. Yeh HW, et al. Red-shifted luciferase-luciferin pairs for enhanced bioluminescence imaging. Nat Methods. 2017 Oct;14(10):971-974.

Background

Diphenylterazine is a bioluminescence agent.

Diphenylterazine is a bioluminescence agent. Diphenylterazine alone yields very little background, leading to excellent signal-to-background ratios. Furthermore, Diphenylterazine elicits minimal cell toxicity at millimolar concentrations. Diphenylterazine injections into untransfected BALB/c mice do not yield any background emission. The bioluminescence resulting from intraperitoneally injected Diphenylterazine displays extended kinetics[1].

[1]. Yeh HW, et al. Red-shifted luciferase-luciferin pairs for enhanced bioluminescence imaging. Nat Methods. 2017 Oct;14(10):971-974.

Chemical Properties

Cas No. 344940-63-2 SDF
Synonyms N/A
Chemical Name N/A
Canonical SMILES O=C1C(CC2=CC=CC=C2)=NC3=C(C4=CC=CC=C4)NC(C5=CC=CC=C5)=CN31
Formula C25H19N3O M.Wt 377.44
Solubility DMSO : ≥ 19 mg/mL (50.34 mM);H2O : < 0.1 mg/mL (insoluble) Storage Store at -20°C, protect from light
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.
Shipping Condition Evaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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