Elastase from porcine pancreas |
| Catalog No.GC61737 |
Elastase from porcine pancreas (PPE), as one of the most prominent serine proteases,it can induce protease antiprotease imbalance and has been applied in an animal model of emphysema and Chronic pulmonary obstructive disease (COPD).
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 39445-21-1
Sample solution is provided at 25 µL, 10mM.
Elastase from porcine pancreas (PPE), as one of the most prominent serine proteases,it can induce protease antiprotease imbalance and has been applied in an animal model of emphysema and Chronic pulmonary obstructive disease (COPD).It has 240 amino acids and a molecular weight of 26 kD. The conserved active site of elastase consists of three residues of serine 214, histidine 71, and aspartate 119 [1-3].
Elastase from porcine pancreas (0-60 mU/ml;24 h) treatment increased placenta growth factor (PlGF) expression and secretion in LE cells[4].
Elastase from porcine pancreas (100 mU/ml; weekly for one month;i.p) instillation increase PlGF expression, activation of JNK and p38 MAPK, and PlGF secretion in the pulmonary cells of mice[4]. Elastase from porcine pancreas can induces emphysema in hamsters[5].
References:
[1]. Shapiro SD. Animal models for COPD. Chest 2000; 117: 223S-227S.
[2]. M. WÜrtele, M. Hahn, K. Hilpert, W. HÖhne. Acta Crystallogr. D Biol. Crystallogr., 56 (2000), p. 520
[3]. Shotton DM, et, al. Amino-acid sequence of porcine pancreatic elastase and its homologies with other serine proteinases. Nature. 1970 Feb 28;225(5235):802-6.
[4]. Hou HH, Cheng SL, et, al. Elastase induced lung epithelial cell apoptosis and emphysema through placenta growth factor. Cell Death Dis. 2013 Sep 5;4(9):e793. doi: 10.1038/cddis.2013.329. PMID: 24008737; PMCID: PMC3789187.
[5]. Stone PJ, Lucey EC, et, al. Lnduction and exacerbation of emphysema in hamsters with human neutrophil elastase inactivated reversibly by a peptide boronic acid. Am Rev Respir Dis. 1990 Jan;141(1):47-52. doi: 10.1164/ajrccm/141.1.47. PMID: 2297186.
| Cell experiment [1]: | |
|
Cell lines |
Mouse LE cells (mouse alveolar type II epithelial cells) |
|
Preparation Method |
Cells were treated with 0-60 mU/ml Elastase from porcine pancreas for 24 h |
|
Reaction Conditions |
0-60 mU/ml Elastase from porcine pancreas ;24 h |
|
Applications |
Elastase from porcine pancreas treatment increased placenta growth factor (PlGF) expression and secretion in LE cells. |
| Animal experiment [2]: | |
|
Animal models |
C57BL/6 mice |
|
Preparation Method |
Mice were intratracheally instillated with saline (CON) or 100 mU/ml Elastase from porcine pancreas weekly for one month. |
|
Dosage form |
100 mU/ml Elastase from porcine pancreas; weekly for one month;i.p |
|
Applications |
Elastase from porcine pancreas instillation increase PlGF expression, activation of JNK and p38 MAPK, and PlGF secretion in the pulmonary cells of mice. |
|
References: [1]. Hou HH, Cheng SL, et, al. Elastase induced lung epithelial cell apoptosis and emphysema through placenta growth factor. Cell Death Dis. 2013 Sep 5;4(9):e793. doi: 10.1038/cddis.2013.329. PMID: 24008737; PMCID: PMC3789187. |
|
| Cas No. | 39445-21-1 | SDF | |
| Formula | C1135H1759N331O346S10 | M.Wt | 25898.13 |
| Solubility | H2O : 50 mg/mL (ultrasonic and adjust pH to 9 with NaOH); H2O : 1 mg/mL (Need ultrasonic) | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 0.0386 mL | 0.1931 mL | 0.3861 mL |
| 5 mM | 0.0077 mL | 0.0386 mL | 0.0772 mL |
| 10 mM | 0.0039 mL | 0.0193 mL | 0.0386 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
-
Biological Activity: 30 U/mg solid
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 21 reference(s) in Google Scholar.)
GLPBIO products are for RESEARCH USE ONLY. Please make sure your review or question is research based.
Required fields are marked with *