GLPBio

CY7

Catalog No.: GC35772Purity:>97.00%
COASDSData Sheet
CY7 is a cyanine-containing fluorescent dye that is commonly used to label proteins, antibodies, and small molecule compounds. Its maximum excitation/emission wavelengths are 745/800nm, respectively.

Products are for research use only. Not for human use. We do not sell to patients.

CY7
Cas No.: 943298-08-6
10mM (in 1mL DMSO)In Stock
$179.00
1
1mgIn Stock
$48.00
1
5mgIn Stock
$119.00
1
10mgIn Stock
$147.00
1
25mgIn Stock
$294.00
1
Tel: (909) 407-4943 / (909) 689-7124Email: sales@glpbio.com

Citations

2 publicationsPublications from researchers using this product

Description

CY7 is a cyanine-containing fluorescent dye that is commonly used to label proteins, antibodies, and small molecule compounds. Its maximum excitation/emission wavelengths are 745/800nm, respectively[1]. The basic structure of CY7 is a cyclic compound with a conjugated double bond system. It has excellent fluorescence properties in the far-red region and good photostability[2]. CY7 is a cyanine compound with the characteristics of long wavelength, adjustable absorption and emission, high extinction coefficient, good water solubility, and relatively simple synthesis[3]. CY7 usually exists in the form of its active ester or azide, which enables it to be labeled with biological molecules such as proteins and nucleic acids through covalent bonds[4]. The optimal molar ratio of CY dye to protein is about 10:1[5]. CY7 has a wide range of applications. It is not only suitable for biomarkers and cell imaging, but also for nucleic acid detection, protein analysis, and drug screening[6]. The molecular weight of this product is 682.85. It is for research purposes only and is not suitable for food, drugs, medical devices, or cosmetics.

References:
[1] Zhang R, Yang J, Radford D C, et al. FRET Imaging of Enzyme‐Responsive HPMA Copolymer Conjugate[J]. Macromolecular bioscience, 2017, 17(1): 1600125.
[2] Yuan L, Lin W, Zheng K, et al. Far-red to near infrared analyte-responsive fluorescent probes based on organic fluorophore platforms for fluorescence imaging[J]. Chemical Society Reviews, 2013, 42(2): 622-661.
[3] Pandey R K, James N, Chen Y, et al. Cyanine dye-based compounds for tumor imaging with and without photodynamic therapy[J]. Heterocyclic Polymethine Dyes: Synthesis, Properties and Applications, 2008: 41-74.
[4] Gerowska M, Hall L, Richardson J, et al. Efficient reverse click labeling of azide oligonucleotides with multiple alkynyl Cy-Dyes applied to the synthesis of HyBeacon probes for genetic analysis[J]. Tetrahedron, 2012, 68(3): 857-864.
[5] Yu H, Chao J, Patek D, et al. Cyanine dye dUTP analogs for enzymatic labeling of DNA probes[J]. Nucleic Acids Research, 1994, 22(15): 3226-3232.
[6] Ntziachristos V, Schellenberger E A, Ripoll J, et al. Visualization of antitumor treatment by means of fluorescence molecular tomography with an annexin V–CY7. 5 conjugate[J]. Proceedings of the National Academy of Sciences, 2004, 101(33): 12294-12299.

Protocol

This protocol only provides a guide, please modify it to meet your specific needs.

1. Solution preparation

(1) Working solution: Dissolve CY7 solid in DMSO to a final concentration of 10mM.

Note: The working solution must be prepared and used immediately. Before use, 500μg/mL condensation solution (Cat. No.: GA11126) must be used for activation before subsequent labeling experiments can be performed.

2. Experimental steps for labeling proteins with CY7

(1) Protein preparation: Dissolve the protein in a pH 8.5 buffer without primary amines (such as Tris or glycine) and ammonium ions, and prepare the concentration to 2-10mg/mL to obtain the best labeling efficiency. If the pH is lower than 8.0, adjust with 1M sodium bicarbonate.

(2) Calculation of the amount of CY7 working solution: The amount of CY7 (MW=682.85) required for the labeling reaction depends on the amount of protein to be labeled. The optimal molar ratio of CY dye to protein is about 10:1.

(3) Labeling reaction: Take the calculated volume of CY7 working solution and slowly add it to the protein sample solution, gently shake to mix, and then briefly centrifuge to collect the sample at the bottom of the reaction tube. Avoid violent mixing to prevent the protein sample from denaturing and inactivating.

(4) Incubation reaction: Place the reaction tube in a dark place and incubate at room temperature or 4°C with gentle shaking for 60min. Every 10-15min, gently invert the reaction tube several times to fully mix the two reactants and improve the labeling efficiency. The labeling time is 2-18h, and the labeling ratio and time are adjusted according to the labeling effect.

Note:

(1)This product is an unactivated fluorescent dye. To use this product to label biological molecules such as peptides and proteins, it must first be activated with carboxylic acid. If an activated form is required, sulfo-Cyanine5 NHS Ester (Cat. No.: GC59174) is recommended.

(2)This protocol provides guidance for using CY7 for protein labeling experiments. It can be adjusted according to other literature and specific experimental requirements. The operation should be carried out in a sterile environment to prevent contaminants from interfering with the reaction. Avoid direct contact with the reaction reagents.

Product Documents

Purity:>97.00%Appearance:A solid
COASDSData Sheet

Chemical Properties

CAS No.
943298-08-6
SMILES
CC(/C(N1CCCCCC(O)=O)=C\C=C\C=C\C=C\C2=[N+](CC)C(C=CC(S(=O)([O-])=O)=C3)=C3C2(C)C)(C)C4=C1C=CC(S(=O)(O)=O)=C4
Molecular Formula
C35H42N2O8S2
Molecular Weight
682.85
Solubility
DMSO: ≥ 33 mg/mL (48.33 mM)
Storage
Store at -20°C, protect from light
General tips
Select a suitable solvent according to the product solubility data. Once prepared, aliquot the stock solution to avoid repeated freeze-thaw cycles.
Use stock solutions within 6 months when stored at -80°C or within 1 month when stored at -20°C.
To improve solubility, warm the tube to 37°C and sonicate it for an appropriate period.
Shipping Condition
Evaluation samples are shipped with blue ice. Other available sizes are shipped at room temperature or with blue ice upon request.

CalculatorsMolarity / Dilution / Molecular Weight / Unit Conversion / In Vivo Formulation / Solubility

g/mol

Reviews

Review for CY7

Rating: 5
  • 5 Star100%
  • 4 Star0%
  • 3 Star0%
  • 2 Star0%
  • 1 Star0%