SN 2 |
Catalog No.GC37655 |
SN 2 is a potent activator of TRPML3 ion channel with an EC50 of 1.8 μM.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 823218-99-1
Sample solution is provided at 25 µL, 10mM.
Quality Control & SDS
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- Purity: >99.50%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
SN 2 is a novel and potent activator of TRPML3 ion channel with EC50 of 1.8±0.13 μM. EC50: 1.8±0.13 μM (TRPML3), >29.9 μM (TRPML1)[1]
The conductance of TRPML3 channels is estimate, when activated with 10 µM SN-2 is approximately 10 pS at -80 mV. TRPML3-expressing HEK293 cells are perfused with a series starting with compound alone (in SBS), with compound in ELS, and finally with ELS alone. Two representative compounds, SF-24 and SN-2, are tested. SF-24 is one of the least effective compounds, and SN-2 is one of the most active ones. SN-2 has a similar synergistic effect, also reaching up-to 10-fold enhancement of the combined response when compared with the individual responses, reaching average current densities of up to 3 nA/pF at -80 mV. Dominant negative TRPML3(D458K) is highly effective in eliminating SN-2-induced activity in epidermal melanocytes, suggesting that SN-2 activates a channel that is not responsive in presence of TRPML3(D458K). Such a dominant negative action might be attributed to potential heteromerization of TRPML3(D458K) with an SN-2-responsive channel[1].
[1]. Grimm C, et al. Small molecule activators of TRPML3. Chem Biol. 2010 Feb 26;17(2):135-48.
Cas No. | 823218-99-1 | SDF | |
Canonical SMILES | CC(C=C(C)C=C1C)=C1C2=NOC3C2C4CC3CC4 | ||
Formula | C17H21NO | M.Wt | 255.35 |
Solubility | DMSO: 100 mg/mL (391.62 mM) | Storage | Store at -20°C |
General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. |
Prepare stock solution | |||
1 mg | 5 mg | 10 mg | |
1 mM | 3.9162 mL | 19.581 mL | 39.1619 mL |
5 mM | 0.7832 mL | 3.9162 mL | 7.8324 mL |
10 mM | 0.3916 mL | 1.9581 mL | 3.9162 mL |
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
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