Soyasapogenol B is a soy-derived component with multiple biological activities. Soyasapogenol B can modulate cellular autophagy, apoptosis, inflammation, and oxidative damage[1-2]. Soyasapogenol B is applicable for research related to colorectal cancer, clear cell renal cell carcinoma, and neurodegenerative diseases[3-4].
In vitro, Soyasapogenol B (0.5-3mM) incubation with Caco-2 cells for 4 hours. Soyasapogenol B significantly inhibited epithelial sodium channel (ENaC)-mediated sodium absorption. The uptake of Soyasapogenol B in Caco-2 cells was concentration-dependent, and Soyasapogenol B inhibited cell viability[5]. Treatment of differentiated 3T3-L1 adipocytes with Soyasapogenol B (6.25-12.5μM) for 10 days. Soyasapogenol B significantly reduced intracellular triglyceride content while increasing the levels of glycerol and free fatty acids in the culture medium[6].
In vivo, dietary administration of Soyasapogenol B (0.02%; mixed in feed) to 12-week-old male C57BL/6 mice for 8 weeks significantly increased the animals' grip strength, exercise endurance, and the weights of the quadriceps and gastrocnemius muscles[7]. In 6-week-old male ICR mice, Soyasapogenol B (10mg/kg) was orally administered once daily for 3 days, starting 24 hours after the seventh intraperitoneal injection of LPS (8μg/kg). Soyasapogenol B significantly restored the LPS-induced impairments in spontaneous alternation behavior and latency time, increased the expression of brain-derived neurotrophic factor (BDNF) and CREB phosphorylation in the hippocampus, and inhibited NF-κB activation and TNF-α expression[8].
References:
[1] Sasaki K, Minowa N, Kuzuhara H, et al. Preventive effects of soyasapogenol B derivatives on liver injury in a concanavalin A-induced hepatitis model. Bioorg Med Chem. 2005 Aug 15;13(16):4900-11.
[2] Yun HM, Lee JY, Kim SH, et al. Effects of Triterpene Soyasapogenol B from Arachis hypogaea (Peanut) on Differentiation, Mineralization, Autophagy, and Necroptosis in Pre-Osteoblasts. Int J Mol Sci. 2022 Jul 27;23(15):8297.
[3] Zhang W, Popovich DG. Effect of soyasapogenol A and soyasapogenol B concentrated extracts on HEP-G2 cell proliferation and apoptosis. J Agric Food Chem. 2008 Apr 23;56(8):2603-8.
[4] Evidente A, Cimmino A, Fernández-Aparicio M, et al. Soyasapogenol B and trans-22-dehydrocam- pesterol from common vetch (Vicia sativa L.) root exudates stimulate broomrape seed germination. Pest Manag Sci. 2011 Aug;67(8):1015-22.
[5] Hu J, Reddy MB, Hendrich S, et al. Soyasaponin I and sapongenol B have limited absorption by Caco-2 intestinal cells and limited bioavailability in women. J Nutr. 2004 Aug;134(8):1867-73.
[6] Iwamoto K, Kamo S, Takada Y, et al. Soyasapogenols reduce cellular triglyceride levels in 3T3-L1 mouse adipocyte cells by accelerating triglyceride lipolysis. Biochem Biophys Rep. 2018 Sep 27;16:44-49.
[7] Ahn BM, Noh Y, Lee JJ, et al. Soyasapogenol B prevents sarcopenia by increasing skeletal muscle mass and function through the Sirt1/PGC-1α and PI3K pathway. Biomed Pharmacother. 2025 Aug;189:118316.
[8] Lee HJ, Lim SM, Ko DB, et al. Soyasapogenol B and Genistein Attenuate Lipopolysaccharide-Induced Memory Impairment in Mice by the Modulation of NF-κB-Mediated BDNF Expression. J Agric Food Chem. 2017 Aug 16;65(32):6877-6885.