Sophoridine (Synonyms: (-)-Sophoridine) |
Catalog No.GC38425
One-Click Copy Product Info
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Sophoridine is a common quateracyclic quinolizidine alkaloid, which can induce apoptosis.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 6882-68-4
Sample solution is provided at 25 µL, 10mM.
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Sophoridine is a common quateracyclic quinolizidine alkaloid, which can induce apoptosis [1]. Sophoridine ameliorates cardiac Ca2+-induced Ca2+ transients through the up-regulation of the L-type Ca2+ channels (LTCCs) and recovery of the sarcoplasmic reticulum calcium capacity by upregulating SERCA2a expression in cardiomyocytes [2]. Sophoridine has been widely used to inhibit the proliferation of various cancer cells and induce DNA breaks [3].
In vitro, Sophoridine treatment for 48 hours significantly inhibited the viability of PANC-1 cells, Miapaca-2 cells, and HepG2 cells, with IC50 values of 19.23µM, 22.65µM, and 26.78µM, respectively[4]. Treatment with 1mg/ml of Sophoridine for 24 hours induced cell cycle arrest in U87MG cells, promoted cell apoptosis, and led to an increase in reactive oxygen species (ROS) production and a decrease in glutathione content[5]. Treatment with 20μg/ml Sophoridine for 24 hours significantly inhibited the migration of NCI-H446 cells and activated the p53 signaling pathway and the Hippo pathway[6].
In vivo, Sophoridine treatment via intragastric administration at a dose of 25mg/kg daily for 25 days significantly inhibited tumor growth in the Lewis tumor-bearing mouse model, with no obvious organ toxicity [7]. Oral administration of 12.15mg/kg/day of Sophoridine for 5 consecutive days significantly reduced paw edema in mice induced by carrageenan, and inhibited the levels of TNF-α, IL-6, PGE2, and IL-8[8].
References:
[1] Tang Q, Liu Y, Peng X, et al. Research progress in the pharmacological activities, toxicities, and pharmacokinetics of sophoridine and its derivatives[J]. Drug Design, Development and Therapy, 2023: 191-212.
[2] Song T, Hao Y, Wang M, et al. Sophoridine manifests as a leading compound for anti-arrhythmia with multiple ion-channel blocking effects[J]. Phytomedicine, 2023, 112: 154688.
[3] ur Rashid H, Rasool S, Ali Y, et al. Anti-cancer potential of sophoridine and its derivatives: Recent progress and future perspectives[J]. Bioorganic chemistry, 2020, 99: 103863.
[4] Xu Z, Zhang F, Bai C, et al. Sophoridine induces apoptosis and S phase arrest via ROS-dependent JNK and ERK activation in human pancreatic cancer cells[J]. Journal of Experimental & Clinical Cancer Research, 2017, 36(1): 124.
[5] Wang W X, Sun Z H, Chen H M, et al. Role and mechanism of Sophoridine on proliferation inhibition in human glioma U87MG cell line[J]. International journal of clinical and experimental medicine, 2015, 8(1): 464.
[6] Zhu L, Huang S, Li J, et al. Sophoridine inhibits lung cancer cell growth and enhances cisplatin sensitivity through activation of the p53 and Hippo signaling pathways[J]. Gene, 2020, 742: 144556.
[7] Zhao B, Hui X, Zeng H, et al. Sophoridine inhibits the tumour growth of non-small lung cancer by inducing macrophages M1 polarisation via MAPK-mediated inflammatory pathway[J]. Frontiers in Oncology, 2021, 11: 634851.
[8] Huang X, Li B, Shen L. Studies on the anti‐inflammatory effect and its mechanisms of sophoridine[J]. Journal of analytical methods in chemistry, 2014, 2014(1): 502626.
| Cell experiment [1]: | |
Cell lines | PANC-1 cells |
Preparation Method | PANC-1 cells were grown in DMEM medium supplied with 10% fetal calf serum (FCS), 100U/ml penicillin, and 100μg/ml streptomycin at 37°C in 5% CO2/atmosphere. Cells (5×103 cells/ml) were seeded in 96-well plates and allowed to adhere in a 5% CO2 incubator at 37°C overnight. Different concentrations of Sophoridine (0, 5, 10, 20, 40, 60, 80, and 100μM) were treated for 48h, and the cell viability was analyzed. |
Reaction Conditions | 0, 5, 10, 20, 40, 60, 80, and 100μM; 48h |
Applications | Sophoridine treatment significantly reduced the cell viability of PANC-1 cells in a dose-dependent manner. |
| Animal experiment [2]: | |
Animal models | Female C57BL/6J mice |
Preparation Method | Female C57BL/6J mice (6-week-old) were routinely maintained in a standard environment with food and water ad libitum. LLC cells (8 × 105 cells) in 0.2ml of PBS were injected subcutaneously into the right back side of the mouse. After 1 day, mice were intragastrically (i.g.) administered with 0.2ml of Sophoridine (25mg/kg/day) for 25 days. Tissue (heart, liver, kidney, and spleen) slides were stained with H&E, and the mouse tumors were measured. |
Dosage form | 25mg/kg/day for 25 days; i.g. |
Applications | Sophoridine treatment significantly inhibited the tumor growth of Lewis-bearing mice without causing toxicity on the heart, liver, kidney, and spleen tissues. |
References: | |
| Cas No. | 6882-68-4 | SDF | |
| Synonyms | (-)-Sophoridine | ||
| Canonical SMILES | O=C1CCC[C@]2([H])[C@@]3([H])CCCN4[C@@]3([H])[C@@](CCC4)([H])CN21 | ||
| Formula | C15H24N2O | M.Wt | 248.36 |
| Solubility | DMSO: 50 mg/mL (201.32 mM) | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 4.0264 mL | 20.1321 mL | 40.2641 mL |
| 5 mM | 805.3 μL | 4.0264 mL | 8.0528 mL |
| 10 mM | 402.6 μL | 2.0132 mL | 4.0264 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
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Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >97.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 4 reference(s) in Google Scholar.)