Validamycin A (Synonyms: Validacin) |
Catalog No.GC45135
One-Click Copy Product Info
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Validamycin A is an agricultural antibiotic fungicide that protects crops from the fungus Rhizoctonia solani by inhibiting trehalase, with an IC50 value of 72μM.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 37248-47-8
Sample solution is provided at 25 µL, 10mM.
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Validamycin A is an agricultural antibiotic fungicide that protects crops from the fungus Rhizoctonia solani by inhibiting trehalase, with an IC50 value of 72μM[1]. Validamycin A also inhibits the growth of Aspergillus flavus (MIC value: 1μg/mL) and is commonly used for controlling diseases such as rice sheath blight and potato black scurf, as well as for studying trehalase activity and trehalose synthesis in biological systems[2,3,4,5].
In vitro, Validamycin A (0.125-1μg/mL), either alone or in combination with amphotericin B (2μg/mL), treatment of human bronchial epithelial cells (BEAS-2B) for 24h did not induce significant cytotoxicity. Validamycin A (1μg/mL) treatment of the Aspergillus flavus ATCC 204304 strain for 24h significantly reduced the adhesion capacity of A. flavus[1]. Validamycin A (1μM) treatment of Rhizoctonia cerealis shake-flask cultures for 8 days significantly reduced the total inositol content in the mycelium (by 62%) and decreased the levels of free and hydrolyzed inositol in the culture supernatant[6].
In vivo, exposure of approximately 100 Aedes aegypti mosquito eggs to rearing medium containing Validamycin A (0.1, 0.2, 0.5mg/mL) in a 200 mL volume, with larvae continuously exposed until the adult stage, significantly delayed hatching, pupation, and eclosion processes, and dose-dependently inhibited the flight ability of adults (100% of adults were unable to fly at the 0.5mg/mL concentration)[7]. Validamycin A (150, 300, 600, 1200mg/kg) co-administered orally with pentylenetetrazol (PTZ) to adult zebrafish, followed by 20min of electroencephalogram (EEG) recording, dose-dependently reduced the number and cumulative duration of PTZ-induced seizure-like EEG activities[8].
References:
[1] PLABOUTONG N, EKRONARONGCHAI S, NIWETBOWORNCHAI N, et al. The inhibitory effect of validamycin A on Aspergillus flavus[J]. International Journal of Microbiology, 2020, 2020(1): 3972415.
[2] ASANO N, YAMAGUCHI T, KAMEDA Y, et al. Effect of validamycins on glycohydrolases of Rhizoctonia solani[J]. The Journal of Antibiotics, 1987, 40(4): 526-532.
[3] GARCÍA M D, ARGÜELLES J C. Trehalase inhibition by validamycin A may be a promising target to design new fungicides and insecticides[J]. Pest Management Science, 2021, 77(9): 3832-3835.
[4] HU F, SU W, LI C, et al. Control Efficacy of Validamycin A (0.2 Billion Spores/ml) Paenibacillus polymyxaDN-1 3% AS for Rice Sheath Blight[J]. Agricultural Science & Technology, 2016, 17(11): 2615.
[5] MATSUURA K. Characteristics of validamycin A in controlling Rhizoctonia diseases[M]//Natural Products. Pergamon, 1983: 301-308.
[6] ROBSON G D, KUHN P J, TRINCI A P J. Effect of validamycin A on the inositol content and branching of Rhizoctonia cerealisand other fungi[J]. Journal of General Microbiology, 1989, 135(4): 739-750.
[7] MARTEN A D, STOTHARD A I, KALERA K, et al. Validamycin A delays development and prevents flight in Aedes aegypti(Diptera: Culicidae)[J]. Journal of Medical Entomology, 2020, 57(4): 1096-1103.
[8] LEE E, BANIK A, LEE K B, et al. Assessment of the novel anti-seizure potential of validamycin A using zebrafish epilepsy model[J]. Molecules, 2024, 29(11): 2572.
| Cell experiment [1]: | |
Cell lines | BEAS-2B cells (human bronchial epithelial cells) |
Preparation Method | BEAS-2B cells were treated with Validamycin A (0.125, 0.5, 1μg/mL) alone or in combination with amphotericin B (2μg/mL) for 24h. The toxicity of Validamycin A and amphotericin B on BEAS-2B cells using Lactate Dehydrogenase (LDH) Cytotoxicity Colorimetric Assay Kit II. After 24h, LDH reaction mixture was added (25μL) and incubated at 37℃ for 30min. Then, ODs were measured at 450nm using a spectrophotometer. |
Reaction Conditions | 0.125, 0.5, 1μg/mL; 24h |
Applications | Treatment with Validamycin A (0.125-1μg/mL) alone or in combination with amphotericin B (2μg/mL) did not produce significant toxicity to BEAS-2B cells. |
| Animal experiment [2]: | |
Animal models | Adult zebrafish |
Preparation Method | Validamycin A (150, 300, 600, 1200mg/kg) was administered along with PTZ into the mouth of adult zebrafish, and EEG activity in the brain was monitored using noninvasive flat electrodes and perfomed for 20min. |
Dosage form | 150, 300, 600, 1200mg/kg; p.o. |
Applications | Treatment with Validamycin A can reduce the number and cumulative duration of PTZ-induced seizure-like EEG activity. |
References: | |
| Cas No. | 37248-47-8 | SDF | |
| Synonyms | Validacin | ||
| Canonical SMILES | O[C@H]([C@H]1O)[C@H](O)[C@@H](CO)O[C@@]1([H])O[C@H]2[C@H](O)[C@@H](O)[C@@H](N[C@@]3([H])C=C(CO)[C@@H](O)[C@H](O)[C@H]3O)C[C@@H]2CO | ||
| Formula | C20H35NO13 | M.Wt | 497.5 |
| Solubility | DMSO: 2 mg/ml,PBS (pH 7.2): 10 mg/ml | Storage | 4°C, protect from light, stored under nitrogen |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 2.0101 mL | 10.0503 mL | 20.1005 mL |
| 5 mM | 402 μL | 2.0101 mL | 4.0201 mL |
| 10 mM | 201 μL | 1.005 mL | 2.0101 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
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Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >60.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 5 reference(s) in Google Scholar.)