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β-Glycerophosphate (sodium salt hydrate)

Catalog No.GC11806

β-Glycerophosphate (sodium salt hydrate) Chemical Structure

A protein phosphatase inhibitor

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Sample solution is provided at 25 µL, 10mM.

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Background

β-Glycerophosphate (sodium salt hydrate) is a potent protein phosphatase inhibitor [1].

Protein phosphatase is an enzyme that removes a phosphate group from the phosphorylated amino acid residue of its target protein. Protein phosphorylation is one of the most common protein posttranslational modifications.

β-Glycerophosphate is a potent protein phosphatase inhibitor that acts as a phosphate group donor in bone cell mineralization studies [1][3]. In MC3T3-E1, ROS 17/2.8, and chick osteoblast-like cells, β-Glycerophosphate did not affect the rate of anaerobic glycolysis, but increased the medium Pi levels, suggesting that β-Glycerophosphate was hydrolyzed by bone cells. The local increase in medium Pi concentration promoted rapid mineral deposition [1]. In human bone marrow stromal cells (HBMSC) and human osteoblasts (HOB), BMP-2 resulted in mineralization of their matrix in the presence of beta-glycerophosphate and ascorbic acid [3]. β-Glycerophosphate (10 mM) also accelerated calcification of cultured vascular smooth muscle cells through an alkaline phosphatase-related mechanism [4].

References:
[1].  Chung CH, Golub EE, Forbes E, et al. Mechanism of action of beta-glycerophosphate on bone cell mineralization. Calcif Tissue Int. 1992 Oct;51(4):305-11.
[2].  Fujimoto H, Mabuchi I. Isolation of cleavage furrows from eggs of regular sea urchins and identification of furrow-specific proteins. J Biochem. 1997 Sep;122(3):518-24.
[3].  Lecanda F, Avioli LV, Cheng SL. Regulation of bone matrix protein expression and induction of differentiation of human osteoblasts and human bone marrow stromal cells by bone morphogenetic protein-2. J Cell Biochem. 1997 Dec 1;67(3):386-96.
[4].  Shioi A, Nishizawa Y, Jono S, et al. Beta-glycerophosphate accelerates calcification in cultured bovine vascular smooth muscle cells. Arterioscler Thromb Vasc Biol. 1995 Nov;15(11):2003-9.

Chemical Properties

Cas No. 13408-09-8 SDF
Synonyms N/A
Chemical Name 2-(dihydrogen phosphate)-1,2,3-propanetriol, disodium salt, pentahydrate
Canonical SMILES [O-]P(OC(CO)CO)([O-])=O.[Na+].[Na+].O.O.O.O.O
Formula C3H7O6P • 2Na [5H2O] M.Wt 306.1
Solubility ≥ 30.7mg/mL in H2O, ≥ 14.3mg/mL in DMSO Storage 4°C, protect from light
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.
Shipping Condition Evaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request

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