CleanCap Reagent AU |
Catalog No.GN20150
One-Click Copy Product Info
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CleanCap Reagent AU is specifically designed for co-transcriptional capping, with the structure m7G(5')ppp(5')(2'OMeA)pU, to produce mRNA with a natural Cap1 structure.
Products are for research use only. Not for human use. We do not sell to patients.
Sample solution is provided at 25 µL, 10mM.
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CleanCap Reagent AU is specifically designed for co-transcriptional capping, with the structure m7G(5')ppp(5')(2'OMeA)pU, to produce mRNA with a natural Cap1 structure[1]. CleanCap Reagent AU is specifically designed for self-replicating RNA based on the genomes of positive-strand RNA viruses (such as Venezuelan equine encephalitis virus (VEEV), Semliki Forest virus (SFV), and Sindbis virus (SIN)), whose positive-strand genomes begin with 5'-AU...[2, 3]. Compared to Cap0 mRNA prepared by traditional co-transcriptional capping methods (such as anti-reverse cap analog, ARCA), mRNA with a Cap1 structure exhibits superior activity, stability, and lower immunogenicity in vivo[4]. CleanCap Reagent AU can be used in conjunction with GlpBio's series of modified NTP products.
References:
[1] Henderson J M, Ujita A, Hill E, et al. Cap 1 messenger RNA synthesis with co‐transcriptional cleancap® analog by in vitro transcription[J]. Current protocols, 2021, 1(2): e39.
[2] Lu X, Wu Y, Zhao C, et al. Co‐expression of HSV‐1 ICP34. 5 enhances the expression of gene delivered by self‐amplifying RNA and mitigates its immunogenicity[J]. FEBS Open Bio, 2025.
[3] Kairuz D, Samudh N, Ely A, et al. Production, characterization, and assessment of permanently cationic and ionizable lipid nanoparticles for use in the delivery of self-amplifying RNA vaccines[J]. Pharmaceutics, 2023, 15(4): 1173.
[4] Zhang H, Ma J, Ma T, et al. GPT-driven generation and biological activity evaluation of novel mRNA trinucleotide Cap1 analogs for mRNA vaccine or immunotherapy[J]. Journal of Materials Chemistry B, 2025.
This protocol only provides a guide, please modify it to meet your specific needs.
CleanCap Reagent AU is used for transcription reactions:
1. Add RNase-free water and NTPs to the reaction tube.
2. Add CleanCap Reagent AU to the tube and vortex to mix. Briefly centrifuge to collect the liquid at the bottom of the tube.
3. Add 10X transcription buffer. Vortex to mix. Briefly centrifuge to collect the liquid at the bottom of the tube.
4. Add DNA template.
5. Add murine RNase inhibitor, yeast inorganic pyrophosphatase, and T7 RNA polymerase.
6. Gently flick or invert the tube 10 times to mix thoroughly, then briefly centrifuge to collect the liquid at the bottom of the tube.
7. Incubate at 37°C for 2-3h.
Note: All reagents must be strictly RNase-free. Use disposable RNase-free tubes and bottles. Surfaces and pipettes can be wiped with RNaseZap to inactivate RNase. If possible, use dedicated RNase-free pipettes. Avoid using pipettes that have been used for RNase A plasmid preparation.
| purity | ＞95.00% | Extinction Coefficient | 33,400 Lmol-1cm-1 at 258 nm |
| Formula | C31H42N12O25P4 (free acid) | M.Wt | 1106.6 g/mole (free acid) |
| Salt Form | Na+ | Concentration | 102mM |
| Buffer | H2O | Storage | -20°C or below, always avoid freeze-thaw cycles. |
| Synonyms | CleanCap AU for Self-Amplifying mRNA, CleanCap Reagent AU for co-transcriptional capping of mRNA, m7G(5')ppp(5')(2'OMeA)pU | Backbone | 5'-5'-Triphosphate |
| Base Analog | Adenosine, Uridine | Sugar Type | RNA |
| Nucleotide Category | Cap analogs | ||
| Applications | CRISPR, In vitro Transcription, Recombinases, TALENS, Transposases, Vaccine Development, Zinc Finger Nucleases | ||
Quality Control & SDS
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Appearance: Clear colorless solution
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 4 reference(s) in Google Scholar.)