Isovitexin |
Catalog No.GN10804
One-Click Copy Product Info
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Isovitexin extracted from rice hulls of Oryza sativa, possesses various biological activities, including anti-inflammatory and antioxidant activities.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 38953-85-4 ; 29702-25-8
Sample solution is provided at 25 µL, 10mM.
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Isovitexin extracted from rice hulls of Oryza sativa, possesses various biological activities, including anti-inflammatory and antioxidant activities[1].
In vitro，Isovitexin (5-20μM; 24h) protected HaCaT cells from H2O2-induced oxidative damage, alleviated H2O2-induced apoptosis[2]. Pretreatment HaCaT cells with 10-50μM Isovitexin for 30min completely abolished PM2.5-induced elevation of ROS levels[3].
In vivo, Isovitexin (50 or 100mg/kg; i.p.; once) pretreatment decreased TNF-α and IL-6 production, ROS generation, and MPO and MDA content, while increased SOD and GSH levels and inhibited the protein expression of iNOS and COX-2 in LPS-induced acute lung injury (ALI) mice[4]. Isovitexin (5mg/kg; oral) restored bone mass and architecture of trabecular bones (femur metaphysis and fifth lumbar vertebra/L5) and cortical bones (femur diaphysis) in ovariectomized (OVX) osteopenic mice. Isovitexin has an oral bioavailability of 14.58%[5].
References:
[1] Kang, Jie, et al. "Flavonoids from acai (Euterpe oleracea Mart.) pulp and their antioxidant and anti-inflammatory activities." Food chemistry 128.1 (2011): 152-157.
[2] Lv, Xuechun, et al. "The anti-apoptosis effect of isovitexin on human keratinocytes by regulating miR-98-5p/Bcl-2/Bcl-xL and MAPKs/NF-κB signaling pathways." Food Chemistry Advances 2 (2023): 100238.
[3] Chowjarean, Verisa, et al. "Isovitexin increases stem cell properties and protects against PM2. 5 in keratinocytes." in vivo 33.6 (2019): 1833-1841.
[4] Lv, Hongming, et al. "Isovitexin exerts anti-inflammatory and anti-oxidant activities on lipopolysaccharide-induced acute lung injury by inhibiting MAPK and NF-κB and activating HO-1/Nrf2 pathways." International journal of biological sciences 12.1 (2016): 72.
[5] Pal, Subhashis, et al. "Oral Administration of Isovitexin, a Naturally Occurring Apigenin Derivative Showed Osteoanabolic Effect in Ovariectomized Mice: A Comparative Study with Teriparatide: S. Pal et al." Calcified Tissue International 111.2 (2022): 196-210.
| Cell experiment [1]: | |
Cell lines | RAW 264.7 |
Preparation Method | RAW 264.7 cells were plated in 96-well plates (1×104 cells/well) and incubated with various concentrations of Isovitexin (final concentration: 0-200μg/ml) and LPS (2μg/ml) for 24h. In addition, the cells were pretreated with Isovitexin (25 or 50μg/ml) for 1h, followed by the addition of H2O2 (300μM). After 24h, cell viability was determined by MTT assay. |
Reaction Conditions | 0-200μg/ml; 24h. |
Applications | 100μg/ml Isovitexin were not toxic to RAW 264.7 cells, but treatment with 200μg/ml Isovitexin caused significant cytotoxicity. The addition of H2O2 markedly reduced cell viability and induced apoptosis, which were attenuated by Isovitexin exposure at 25 and 50μg/ml. |
| Animal experiment [1]: | |
Animal models | 6-8 weeks old male BALB/c mice |
Preparation Method | Mice were randomly divided into six groups: control (saline), Isovitexin only (100mg/kg, dissolved in 0.5% DMSO), LPS only (0.5mg/kg, dissolved in saline), LPS (0.5mg/kg) + Isovitexin (50 or 100mg/kg), and LPS (0.5mg/kg) + dexamethasone (Dex, 5mg/kg dissolved in saline). Isovitexin (50 or 100mg/kg) or Dex (5mg/kg) were administered intraperitoneally. After exposure to Isovitexin or Dex for 1h, the mice were anesthetized with diethyl ether, and LPS was administered intranasally (i.n.) to induce lung injury. After LPS administration for 12h, the animals were euthanized. |
Dosage form | 50 or 100mg/kg; i.p.; once. |
Applications | Isovitexin pretreatment decreased TNF-α and IL-6 production, ROS generation, and MPO and MDA content, while increased SOD and GSH levels and inhibited the protein expression of iNOS and COX-2 in LPS-induced ALI mice. |
References: | |
| Cas No. | 38953-85-4 ; 29702-25-8 | SDF | |
| Chemical Name | 5,7-dihydroxy-2-(4-hydroxyphenyl)-6-[(2S,3R,4R,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]chromen-4-one | ||
| Canonical SMILES | C1=CC(=CC=C1C2=CC(=O)C3=C(C(=C(C=C3O2)O)C4C(C(C(C(O4)CO)O)O)O)O)O | ||
| Formula | C21H20O10 | M.Wt | 432.38 |
| Solubility | DMF: 30 mg/ml,DMSO: 30 mg/ml,DMSO:PBS (pH 7.2) (1:5): 0.16 mg/ml | Storage | Store at 2-8°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.3128 mL | 11.5639 mL | 23.1278 mL |
| 5 mM | 462.6 μL | 2.3128 mL | 4.6256 mL |
| 10 mM | 231.3 μL | 1.1564 mL | 2.3128 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)