Peimisine |
Catalog No.GN10319
One-Click Copy Product Info
|
Peimisine is a kind of bioactive alkaloids found in Fritillaria bulbs which can inhibit angiotensin converting enzyme (ACE) activity with an IC50 of 526.5μM.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 19773-24-1
Sample solution is provided at 25 µL, 10mM.
_1-3.$$$39978408@51.jpg');)
_1-9.$$$38538795@65.jpg');)
_1-9.$$$39112701@51.jpg');)
_1-7.$$$37316672@70.jpg');)
_366-372.$$$36198801@70.jpg');)
.$$$38565142@65.jpg');)
_1867-1883.$$$33248023@67.jpg');)
_eads6055.$$$39977546@45.jpg');)
_eadm9805.$$$40080571@45.jpg');)
_eadj3020.$$$39666821@45.jpg');)
_1-20.$$$39757301@28.jpg');)
_1-24.$$$38898113@28.jpg');)
_273-287.$$$36806353@46.jpg');)
_1-16.$$$37156877@44.jpg');)
_1-19.$$$37460805@44.jpg');)
_1291-1307.$$$34518654@46.jpg');)
Peimisine is a kind of bioactive alkaloids found in Fritillaria bulbs which can inhibit angiotensin converting enzyme (ACE) activity with an IC50 of 526.5μM [1]. Peimisine has anti-acute lung injury effect and has obvious cytotoxicity against various tumor cells [2, 3].
Peimisine (10, 20 and 40μM; 24h) reduced the reactive oxygen species (ROS), malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE) content and restored the level of superoxide dismutase (SOD) in cigarette smoke extract (CSE)-induced BEAS-2B cells; Peimisine (10, 20 and 40μM; 24h) significantly decreased the number of apoptotic cells and the cell apoptosis rate in CSE-induced BEAS-2B cells [4]. Peimisine (160μM; 24h) significantly upregulated the expression of ADRB2 and NOS3 and downregulated the expression of SRC and MMP2 in BEAS-2B cells stimulated with CSE [5].
Peimisine (5 and 15mg/kg; 7d; p.o.) significantly alleviated the disease signs of colitis C57BL/C mice; Peimisine (5 and 15mg/kg; 7d; p.o.) significantly downregulated the expression levels of CD68 and CD11b in colitis C57BL/C mice [6].
References:
[1] Oh H, Kang D G, Lee S, et al. Angiotensin converting enzyme (ACE) inhibitory alkaloids from Fritillaria ussuriensis [J]. Planta medica, 2003, 69(6): 564-565.
[2] Jin X, Gao X, Lan M, et al. Study the mechanism of peimisine derivatives on NF-κB inflammation pathway on mice with acute lung injury induced by lipopolysaccharide [J]. Chemical biology & drug design, 2022, 99(5): 717-726.
[3] Wang D, Jiang Y, Wu K, et al. Evaluation of antitumor property of extracts and steroidal alkaloids from the cultivated Bulbus Fritillariae ussuriensis and preliminary investigation of its mechanism of action [J]. BMC complementary and alternative medicine, 2015, 15: 29.
[4] Liu C, Zhu X, Aga E, et al. Ebeiedinone and peimisine inhibit cigarette smoke extract-induced oxidative stress injury and apoptosis in BEAS-2B cells [J]. Cell stress & chaperones, 2024, 29(6): 697-708.
[5] Xian M, Xu J, Zheng Y, et al. Network pharmacology and experimental verification reveal the regulatory mechanism of Chuanbeimu in treating chronic obstructive pulmonary disease [J]. International journal of chronic obstructive pulmonary disease, 2024, 19: 799-813.
[6] Li Y, Yang X, Han J, et al. Peimisine ameliorates DSS-induced colitis by suppressing Jak-Stat activation and alleviating gut microbiota dysbiosis in mice [J]. The Journal of pharmacy and pharmacology, 2024, 76(5): 545-558.
| Cell experiment [1]: | |
Cell lines | BEAS-2B cells |
Preparation Method | BEAS-2B cells were stimulated with cigarette smoke extract (CSE) to induce oxidative stress injury. Cells were co-incubated with 0.5% CSE and peimisine (10, 20, and 40μM) for 24h and then the reactive oxygen species (ROS), malondialdehyde (MDA), superoxide dismutase (SOD) levels were measured using the kits and the level of 4-hydroxynonenal (4-HNE) was determined by enzyme-linked immunosorbent assay. |
Reaction Conditions | 10, 20 and 40μM; 24h |
Applications | Peimisine reduced the ROS, MDA and 4-HNE content and restored the level of SOD. |
| Animal experiment [2]: | |
Animal models | Male C57BL/C mice |
Preparation Method | Mice were received Peimisine (5 and 15mg/kg) via oral administration for 1 week before Dextran Sulfate Sodium (DSS) stimulation. All groups were subsequently treated with 2.5% DSS for 7 days to induce colitis. The disease activity index (DAI) was determined from days 0 to 7. DAI is the sum of scores for stool consistency, weight loss, and gross bleeding extent. |
Dosage form | 5 and 15mg/kg; 7d; p.o. |
Applications | Peimisine significantly alleviated the disease signs of colitis induced by DSS. |
References: | |
| Cas No. | 19773-24-1 | SDF | |
| Chemical Name | 3,9-bis(azepan-1-yl)-2,4,8,10-tetraoxa-3ж╦5,9ж╦5-diphosphaspiro[5.5]undecane 3,9-dioxide | ||
| Canonical SMILES | C1CCCN(CC1)P2(=O)OCC3(CO2)COP(=O)(OC3)N4CCCCCC4 | ||
| Formula | C27H41NO3 | M.Wt | 427.31 |
| Solubility | ≥ 50mg/mL in DMSO with ultrasonic and warming | Storage | Store at 2-8°C, protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 2.3402 mL | 11.7011 mL | 23.4022 mL |
| 5 mM | 468 μL | 2.3402 mL | 4.6804 mL |
| 10 mM | 234 μL | 1.1701 mL | 2.3402 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)