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[Sar9,Met(O2)11]-Substance P

Catalog No.GC13610

NK1 tachykinin receptor agonist

Products are for research use only. Not for human use. We do not sell to patients.

[Sar9,Met(O2)11]-Substance P Chemical Structure

Cas No.: 110880-55-2

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1mg
$92.00
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Sample solution is provided at 25 µL, 10mM.

Product Documents

Quality Control & SDS

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Protocol

Kinase experiment:

Rats initially receive an i.c.v. injection of artificial cerebrospinal fluid (aCSF; 1 μl) followed 60 min later by a single dose of either [Sar9,Met(O2)11]-Substance P (10 pmol (n=9), 25 pmol (n=9), 65 pmol (n=8) or 100 pmol (n=8)) or septide (10 pmol (n=12), 25 pmol (n=9), 65 pmol (n=6) or 100 pmol (n=6)) to construct a complete dose-response curve. Each rat is selected randomly and injected with only one of the two agonists for the remainder of the protocol. Increasing doses of [Sar9,Met(O2)11]-Substance P or septide are given at 24 h intervals on day 1 (10 pmol), day 2 (25 pmol), day 3 (65 pmol) and day 4 (100 pmol). Control rats (n=18) receive only the vehicle (aCSF) each day of experiment. Peptides are administered in a volume of 1 μL of vehicle followed by 5 μL flush volume of aCSF which corresponds to the void volume of the catheter. Each dose is calculated per rat in 1 μL solution[1].

References:

[1]. Cellier E, et al. Characterization of central and peripheral effects of septide with the use of five tachykinin NK1 receptor antagonists in the rat. Br J Pharmacol. 1999 Jun;127(3):717-28.

Background

[Sar9,Met(O2)11]-Substance P is a tachykinin NK1 receptor selective agonist.
[Sar9,Met(O2)11]-Substance P and septide (10-100 pmol per rat, i.c.v.) are equipotent in increasing mean arterial blood pressure (MAP) and heart rate (HR), yet they have dissimilar time-course. Both agonists increase dose-dependently face washing and sniffing while [Sar9,Met(O2)11]-Substance P is the sole to produce grooming[1].
Reference:
[1]. Cellier E, et al. Characterization of central and peripheral effects of septide with the use of five tachykinin NK1 receptor antagonists in the rat. Br J Pharmacol. 1999 Jun;127(3):717-28.

Chemical Properties

Cas No. 110880-55-2 SDF
Canonical SMILES CC(C[C@@](/N=C(O)/CN(C([C@](/N=C(O)/[C@](/N=C(O)/[C@](/N=C(O)/[C@](/N=C(O)/[C@]1([H])CCCN1C([C@](/N=C(O)/[C@]2([H])CCCN2C([C@](N)([H])CCCNC(N)=N)=O)([H])CCCCN)=O)([H])CCC(O)=N)([H])CCC(O)=N)([H])CC3=CC=CC=C3)([H])CC4=CC=CC=C4)=O)C)([H])/C(O)=N/[C@@](C(O)=
Formula C64H100N18O15S M.Wt 1393
Solubility Soluble to 1 mg/ml in sterile water Storage Desiccate at -20°C
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table

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1 mg 5 mg 10 mg
1 mM 0.7179 mL 3.5894 mL 7.1788 mL
5 mM 0.1436 mL 0.7179 mL 1.4358 mL
10 mM 0.0718 mL 0.3589 mL 0.7179 mL
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Review for [Sar9,Met(O2)11]-Substance P

Average Rating: 5 ★★★★★ (Based on Reviews and 16 reference(s) in Google Scholar.)

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