GLPBio

Scutellarin

Catalog No.: GN10624Purity:>98.00%Synonyms: Scutellarein 7-O-β-D-glucuronide
COASDSData Sheet
Scutellarin is a flavonoid with multiple biological activities.

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Scutellarin
Cas No.: 27740-01-8
10mM (in 1mL DMSO)In Stock
$35.00
1
10mgIn Stock
$32.00
1
25mgIn Stock
$55.00
1
50mgIn Stock
$98.00
1
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Citations

1 publicationsPublications from researchers using this product

Description

Scutellarin is a flavonoid with multiple biological activities[1]. Scutellarin can prevent endothelial dysfunction and prevent atherosclerosis through antioxidant activity[2]. Scutellarin has anti-HIV-1IIIB, HIV-1(74V) and HIV-1KM018 activities with EC50 of 26μM, 253μM and 136μM, respectively[3].

In vitro, Scutellarin (0-210μM) treated human renal cancer cells (ACHN and 786-O cells) for 24-72h inhibited cell proliferation in a dose- and time-dependent manner, induced cell apoptosis and cell cycle arrest at the G0/G1 phase, and increased the expression of phosphatase and tensin homolog (PTEN)[4]. Scutellarin (50μM) pre-treated BV-2 cells for 30min inhibited the production of NO, TNFα, IL-1β and ROS induced by lipopolysaccharide (LPS), and inhibited LPS-induced nuclear translocation and DNA binding activity of nuclear factor κB (NF-κB)[5].

In vivo, Scutellarin (30, 60mg/kg/day) was orally treated for 3 weeks in mice with H1975-Luciferase cell xenografts, which significantly inhibited the growth of transplanted tumors in mice, upregulated the levels of LC3-II and p-ERK1/2 in mouse lung tissue, and downregulated the level of p-AKT[6]. Scutellarin (5mg/kg) was intravenously injected to treat doxorubicin (DOX)-induced cardiac toxicity in rats, significantly reducing serum lactate dehydrogenase (LDH) activity, lipid peroxide (MDA) levels, and cardiac troponin T (cTnT) concentrations, and significantly reducing cardiac tissue damage[7].

References:
[1] Wang Z L, Wang S, Kuang Y, et al. A comprehensive review on phytochemistry, pharmacology, and flavonoid biosynthesis of Scutellaria baicalensis[J]. Pharmaceutical biology, 2018, 56(1): 465-484.
[2] Mo J, Yang R, Li F, et al. Scutellarin protects against vascular endothelial dysfunction and prevents atherosclerosis via antioxidation[J]. Phytomedicine, 2018, 42: 66-74.
[3] Zhang G H, Wang Q, Chen J J, et al. The anti-HIV-1 effect of scutellarin[J]. Biochemical and Biophysical Research Communications, 2005, 334(3): 812-816.
[4] Deng W, Han W, Fan T, et al. Scutellarin inhibits human renal cancer cell proliferation and migration via upregulation of PTEN[J]. Biomedicine & Pharmacotherapy, 2018, 107: 1505-1513.
[5] Wang S, Wang H, Guo H, et al. Neuroprotection of Scutellarin is mediated by inhibition of microglial inflammatory activation[J]. Neuroscience, 2011, 185: 150-160.
[6] Sun C Y, Li C Y, Li X F, et al. Scutellarin induces apoptosis and autophagy in NSCLC cells through ERK1/2 and AKT Signaling Pathways in vitro and in vivo[J]. Journal of Cancer, 2018, 9(18): 3247.
[7] Sun X P, Wan L L, Yang Q J, et al. Scutellarin protects against doxorubicin-induced acute cardiotoxicity and regulates its accumulation in the heart[J]. Archives of pharmacal research, 2017, 40: 875-883.

Protocol

Cell experiment []:

Cell lines

ACHN、786-O cells

Preparation Method

ACHN and 786-O cells were seeded into 96-well plates at the concentration of 5×103 cells per well and cultured to 70–80% confluence, cells were treated with different concentrations (0, 30, 60, 90, 120, 150, 180, and 210μM) of Scutellarin dissolved in DMSO for 24, 48, 72h at the indicated time point, 10μl of CCK-8 solution was added into each well and incubated for 2h.

Reaction Conditions

0, 30, 60, 90, 120, 150, 180, 210μM; 24, 48, 72h

Applications

Scutellarin inhibites cell proliferation in dose- and time- dependent manner.

Animal experiment []:

Animal models

BALB/c nude mice

Preparation Method

Mice were subcutaneously injected with 4×106 H1975-Luciferase cells (stably expressing fluorescence). When tumor reached approximately 100mm3, mice were randomly divided into three groups (n=8): the vehicle; the low dose Scutellarin (30mg/kg); the high dose Scutellarin (60mg/kg). At this point, Scutellarin was dissolved in PBS, and was orally administered to each mouse once a day for three weeks. The tumor dimensions were measured using digital calliper every 3 days. Before treatment or after the last administration, the tumor size was monitored by in vivo bioluminescence imaging. After 3 weeks, all mice were humanely sacrificed and the tumors were resected for protein quantitation analysis.

Dosage form

30, 60mg/kg/day for 3 weeks; p.o.

Applications

Scutellarin suppressed tumor growth in mouse xenograft model. Scutellarin treatment could up-regulate LC3-II and p-ERK1/2 level, and down-regulate p-AKT.

References:
[1]Deng W, Han W, Fan T, et al. Scutellarin inhibits human renal cancer cell proliferation and migration via upregulation of PTEN[J]. Biomedicine & Pharmacotherapy, 2018, 107: 1505-1513.
[2]Sun C Y, Li C Y, Li X F, et al. Scutellarin induces apoptosis and autophagy in NSCLC cells through ERK1/2 and AKT Signaling Pathways in vitro and in vivo[J]. Journal of Cancer, 2018, 9(18): 3247.

Product Documents

Purity:>98.00%Appearance:A solid
COASDSData Sheet

Chemical Properties

CAS No.
27740-01-8
Synonyms
Scutellarein 7-O-β-D-glucuronide
Chemical Name
(2S,3S,4S,5R,6S)-6-[5,6-dihydroxy-2-(4-hydroxyphenyl)-4-oxochromen-7-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid
SMILES
C1=CC(=CC=C1C2=CC(=O)C3=C(C(=C(C=C3O2)OC4C(C(C(C(O4)C(=O)O)O)O)O)O)O)O
Molecular Formula
C21H18O12
Molecular Weight
462.37
Solubility
≥ 46.2mg/mL in DMSO
Storage
Store at 2-8°C, protect from light
General tips
Select a suitable solvent according to the product solubility data. Once prepared, aliquot the stock solution to avoid repeated freeze-thaw cycles.
Use stock solutions within 6 months when stored at -80°C or within 1 month when stored at -20°C.
To improve solubility, warm the tube to 37°C and sonicate it for an appropriate period.
Shipping Condition
Evaluation samples are shipped with blue ice. Other available sizes are shipped at room temperature or with blue ice upon request.

CalculatorsMolarity / Dilution / Molecular Weight / Unit Conversion / In Vivo Formulation / Solubility

g/mol

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