STh |
Catalog No.GC61489
One-Click Copy Product Info
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STh is a small, heat-stable enterotoxin produced by enterotoxin-induced Escherichia coli (ETEC). STh is a 19-amino acid polypeptide containing three disulfide bridges.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 118447-40-8
Sample solution is provided at 25 µL, 10mM.
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STh is a small, heat-stable enterotoxin produced by enterotoxin-induced Escherichia coli (ETEC). STh is a 19-amino acid polypeptide containing three disulfide bridges[1, 2]. STh stimulates an increase in intracellular cGMP levels by binding to intestinal guanylate cyclase C on the surface of small intestinal epithelial cells, leading to increased intestinal fluid secretion and electrolyte imbalance, thereby causing secretory diarrhea[3]. STh is often used in vaccine development, but its low immunogenicity and small size present challenges, requiring the use of proteins or chemical binding to generate an immune response[4].
References:
[1] Shimonishi Y, Hidaka Y, Koizumi M, et al. Mode of disulfide bond formation of a heat‐stable enterotoxin (STh) produced by a human strain of enterotoxigenic Escherichia coli[J]. FEBS letters, 1987, 215(1): 165-170.
[2] Dubreuil J D. Escherichia coli heat-stable enterotoxins[J]. The Comprehensive Sourcebook of Bacterial Protein Toxins; Elsevier: Amsterdam, The Netherlands, 2015: 874-910.
[3] Prasad H, Mathew J K K, Visweswariah S S. Receptor guanylyl cyclase C and cyclic GMP in health and disease: perspectives and therapeutic opportunities[J]. Frontiers in Endocrinology, 2022, 13: 911459.
[4] Wang H, Zhong Z, Luo Y, et al. Heat-stable enterotoxins of enterotoxigenic Escherichia coli and their impact on host immunity[J]. Toxins, 2019, 11(1): 24.
| Cas No. | 118447-40-8 | SDF | |
| Formula | C79H112N22O30S6 | M.Wt | 2042.25 |
| Solubility | Storage | Store at -20°C | |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 489.7 μL | 2.4483 mL | 4.8966 mL |
| 5 mM | 97.9 μL | 489.7 μL | 979.3 μL |
| 10 mM | 49 μL | 244.8 μL | 489.7 μL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 19 reference(s) in Google Scholar.)