|SV40 large T antigen NLS Catalog No.GC34257|
Sample solution is provided at 25 µL, 10mM.
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|Cas No.||N/A||SDF||Download SDF|
|Solubility||Soluble in H2O||Storage||Store at -20°C|
|General tips||For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.|
|Shipping Condition||Evaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request
SV40 large T antigen NLS is from Large T antigen residue 47 to 55, enables protein import into cell nucleus.
SV40 large tumor-antigen (T-ag) nuclear import is enhanced by the protein kinase CK2 (CK2) site (Ser111Ser112) flanking the nuclear localization sequence (NLS). T-ag NLS can enhance nuclear import, despite the fact that PK-C has been shown to inhibit nuclear import of proteins such as lamin B2.
. Xiao CY, et al. Negative charge at the protein kinase CK2 site enhances recognition of the SV40 large T-antigen NLS by importin: effect of conformation. FEBS Lett. 1998 Dec 4;440(3):297-301. . Xiao CY, et al. An engineered site for protein kinase C flanking the SV40 large T-antigen NLS confers phorbol ester-inducible nuclear import. FEBS Lett. 1998 Oct 9;436(3):313-7.