TSQ |
Catalog No.GC66458
One-Click Copy Product Info
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TSQ is a fluorescent probe that images zinc proteins in cells, with maximum excitation/emission wavelengths near 360nm/470nm.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 109628-27-5
Sample solution is provided at 25 µL, 10mM.
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TSQ is a fluorescent probe that images zinc proteins in cells, with maximum excitation/emission wavelengths near 360nm/470nm[1]. TSQ was successfully employed in pinpoint a pool of free Zn2+ in the brain[2]. TSQ functions as an effective tool for evaluation of Zn2+ within artificial membranes and sperm cells while demonstrating strong binding to lipid bilayers[3]. TSQ can be used to detect free zinc in breast cells (MCF7, MDA-MB-231, T47D, and MCF10A cells) and to determine the distribution and uptake of zinc[4]. TSQ can be used for histochemical staining of tissue sections, which can show the location of zinc-containing (glutamatergic) nerve endings in neurofibroma[5].
References:
[1] Meeusen J W, Tomasiewicz H, Nowakowski A, et al. TSQ (6-methoxy-8-p-toluenesulfonamido-quinoline), a common fluorescent sensor for cellular zinc, images zinc proteins[J]. Inorganic chemistry, 2011, 50(16): 7563-7573.
[2] Frederickson C J, Kasarskis E J, Ringo D, et al. A quinoline fluorescence method for visualizing and assaying the histochemically reactive zinc (bouton zinc) in the brain[J]. Journal of neuroscience methods, 1987, 20(2): 91-103.
[3] Andrews J C, Nolan J P, Hammerstedt R H, et al. Characterization of N‐(6‐methoxy‐8‐quinolyl)‐p‐toluenesulfonamide for the detection of zinc in living sperm cells[J]. Cytometry: The Journal of the International Society for Analytical Cytology, 1995, 21(2): 153-159.
[4] Hübner C, Keil C, Jürgensen A, et al. Comparison of three low-molecular-weight fluorescent probes for measuring free zinc levels in cultured mammary cells[J]. Nutrients, 2023, 15(8): 1873.
[5] Frederickson C J, Suh S W, Silva D, et al. Importance of zinc in the central nervous system: the zinc-containing neuron[J]. The Journal of nutrition, 2000, 130(5): 1471S-1483S.
This protocol only provides a guideline, and should be modified according to your specific needs.
1. Preparation of Staining Solution
(1) Stock Solution Preparation:
Dissolve TSQ in DMSO to prepare a stock solution with a concentration of 1-5mmol/L.
Note: Aliquot unused stock solution and store at -20°C or -80°C, protected from light. Avoid repeated freeze-thaw cycles.
(2) Working Solution Preparation:
Dilute the stock solution with an appropriate buffer (e.g., serum-free medium or PBS) to prepare a working solution with a concentration of 0.1-100μmol/L.
Note: Adjust the working solution concentration as needed. Prepare fresh before use.
2. Staining of Suspension Cells
(1) Suspension Cells:
Centrifuge at 4°C, 1000×g for 3-5 minutes, discard the supernatant, and wash twice with PBS (5 minutes each).
(2) Adherent Cells:
Wash twice with PBS, then digest the cells with trypsin. After digestion, centrifuge at 1000×g for 3-5 minutes.
(3) Resuspend the cells in 1mL of TSQ working solution and incubate at 37°C in the dark for approximately 30 minutes.
Note: Optimal incubation time varies by cell type. Optimize based on experimental requirements.
(4) After incubation, centrifuge at 1000×g for 5 minutes, discard the supernatant, and wash 2-3 times with PBS (5 minutes each).
(5) Resuspend the cells in pre-warmed serum-free medium or PBS and observe using fluorescence microscopy or flow cytometry.
3. Staining of Adherent Cells
(1) Culture adherent cells on sterile coverslips.
(2) Remove the coverslip from the medium, aspirate excess medium, and place the coverslip in a humid environment.
(3) Add 100μL of the dye working solution to one corner of the coverslip and gently tilt to ensure even coverage of all cells.
(4) Incubate at 37°C in the dark for 30 minutes.
Note: Optimal incubation time varies by cell type. Optimize based on experimental requirements.
(5) After incubation, aspirate the dye working solution and wash the coverslip 2-3 times with pre-warmed culture medium.
4. Microscopy Detection
The maximum excitation/emission wavelengths of TSQ are approximately 360nm/470nm.
Reference:
[1] Meeusen J W, Tomasiewicz H, Nowakowski A, et al. TSQ (6-methoxy-8-p-toluenesulfonamido-quinoline), a common fluorescent sensor for cellular zinc, images zinc proteins[J]. Inorganic chemistry, 2011, 50(16): 7563-7573.
| Cas No. | 109628-27-5 | SDF | |
| Formula | C17H16N2O3S | M.Wt | 328.39 |
| Solubility | DMSO : ≥ 100 mg/mL (304.52 mM) | Storage | 4°C, protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 3.0452 mL | 15.2258 mL | 30.4516 mL |
| 5 mM | 609 μL | 3.0452 mL | 6.0903 mL |
| 10 mM | 304.5 μL | 1.5226 mL | 3.0452 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)