5-fluoro Orotic Acid (hydrate) (Synonyms: 5-FOA, 5-fluoro OA) |
| Catalog No.GC42523 |
5-fluoro Orotic acid (5-FOA) is used to detect URA3 gene expression in yeast molecular genetic constructs.
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Cas No.: 220141-70-8
Sample solution is provided at 25 µL, 10mM.
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5-fluoro Orotic acid (5-FOA) is used to detect URA3 gene expression in yeast molecular genetic constructs. Yeast with an active URA3 gene, which encodes orotine-5'-monophosphate decarboxylase, converts 5-FOA to fluorodeoxyuridine, which is toxic to cells. Resistant strains of yeast that carry a mutation in the URA3 gene grow in the presence of 5-FOA if the media is supplemented with uracil.
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