Ac-DEVD-CHO |
| Catalog No.GC32695 |
Ac-DEVD-CHO is a Caspase-3 inhibitor with an IC50 value of 0.016μM.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 169332-60-9
Sample solution is provided at 25 µL, 10mM.
Ac-DEVD-CHO is a Caspase-3 inhibitor with an IC50 value of 0.016μM[1]. Ac-DEVD-CHO also has inhibitory activity against other caspase family members, but is more selective for caspase-3, with a Ki value of 0.23nM [2]. Ac-DEVD-CHO inhibits the apoptosis process by inhibiting the activity of caspases, and can be used to study the apoptosis process and related diseases, such as retinal degeneration[3].
In vitro, Ac-DEVD-CHO (10μM) treated osteoclasts (OCLs) for 24 h, partially blocking sinomenine-induced apoptosis and reducing the number of apoptotic nuclei [4]. Ac-DEVD-CHO (100μM) treated vascular smooth muscle cells for 48 hours, significantly reducing the expression level of caspase-3 and inhibiting artesunate-induced apoptosis [5].
In vivo, Ac-DEVD-CHO (2mg/kg) treated mice with hereditary retinitis through intraperitoneal injection, effectively inhibiting photoreceptor cell apoptosis and temporarily delaying retinal degeneration[6]. Ac-DEVD-CHO (4mg/kg) was injected subcutaneously into mice with acute kidney injury and significantly reduced serum BUN, TNF-α, IL-6, IL-10 concentrations and renal cell apoptosis rate [7].
References:
[1] Firoozpour L, Gao L, Moghimi S, et al. Efficient synthesis, biological evaluation, and docking study of isatin based derivatives as caspase inhibitors[J]. Journal of enzyme inhibition and medicinal chemistry, 2020, 35(1): 1674-1684.
[2] Garcia-Calvo M, Peterson E P, Leiting B, et al. Inhibition of human caspases by peptide-based and macromolecular inhibitors[J]. Journal of Biological Chemistry, 1998, 273(49): 32608-32613.
[3] Yao K, Wang K, Xu W, et al. Caspase-3 and its inhibitor Ac-DEVD-CHO in rat lens epithelial cell apoptosis induced by hydrogen in vitro[J]. Chinese medical journal, 2003, 116(07): 1034-1038.
[4] He L, Li X, Zeng X, et al. Sinomenine induces apoptosis in RAW 264.7 cell-derived osteoclasts in vitro via caspase-3 activation[J]. Acta Pharmacologica Sinica, 2014, 35(2): 203-210.
[5] Zhang J, Wang L, Chen H, et al. Effect of Caspase Inhibitor Ac-DEVD-CHO on Apoptosis of Vascular Smooth Muscle Cells Induced by Artesunate[J]. AIMS Bioengineering, 2014, 1(1): 13-24.
[6] Yoshizawa K, Kiuchi K, Nambu H, et al. Caspase-3 inhibitor transiently delays inherited retinal degeneration in C3H mice carrying the rd gene[J]. Graefe's archive for clinical and experimental ophthalmology, 2002, 240: 214-219.
[7] Liu L X, Hu Z J, Li Y, et al. The effect of caspase-3 inhibitor on the concentrations of serum inflammatory cytokines in sepsis related acute kidney injury induced by peritoneal cavity infection in mice[J]. Zhongguo wei Zhong Bing ji jiu yi xue, 2010, 22(12): 736-739.
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Cell experiment [1]: |
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Cell lines |
OCLs |
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Preparation method |
OCLs were incubated with RANKL and treated with 0.5 mmol/L SIN with or without the specific caspase-3 inhibitor Ac-DEVD-CHO (10μM) for 24 h. |
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Reaction Conditions |
10μM; 24 h |
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Applications |
Ac-DEVD-CHO partially blocked the effect of SIN-induced apoptosis and reduced the number of apoptotic nuclei. |
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Animal experiment [2]: |
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Animal models |
C3H and ICR mice |
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Preparation method |
Starting at 8 days of age, 40 C3H and 40 ICR pups received an i.p. injection of 2 mg/kg body weight of Ac-DEVD-CHO or saline every other day.Ten C3H and ICR mice per group were randomly selected and killed at 13 days of age, and the remaining 10 C3H and ICR mice in each group were killed at 17 days. |
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Dosage form |
2mg/kg; i.p. |
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Applications |
Ac-DEVD-CHO transiently delays inherited retinal degeneration in C3H mice carrying the rd gene. |
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References: |
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| Cas No. | 169332-60-9 | SDF | |
| Canonical SMILES | N-Acetyl-Asp-Glu-Val-Asp-al | ||
| Formula | C20H30N4O11 | M.Wt | 502.47 |
| Solubility | Water : ≥ 50 mg/mL (99.51 mM) | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.9902 mL | 9.9508 mL | 19.9017 mL |
| 5 mM | 0.398 mL | 1.9902 mL | 3.9803 mL |
| 10 mM | 0.199 mL | 0.9951 mL | 1.9902 mL |
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 39 reference(s) in Google Scholar.)
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