Asiaticoside (Synonyms: Ba 2742, NSC 36002, NSC 166062) |
| Catalog No.GN10534 |
Asiaticoside is a pentacyclic triterpenoid glycoside extracted from Centella asiatica.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 16830-15-2
Sample solution is provided at 25 µL, 10mM.
Asiaticoside is a pentacyclic triterpenoid glycoside extracted from Centella asiatica [1]. Asiaticoside activates the Smad signaling pathway (specifically in a TGF-β receptor I kinase-independent manner), promoting the synthesis of type I collagen by fibroblasts, thereby strengthening skin structure and wound healing [2-3]. Asiaticoside is commonly used in wound healing and skin disease treatment [4].
In HCT116 cells, Asiaticoside (0.1-2µM; 24-72h) significantly reduced cell viability in a time- and dose-dependent manner [5]. In human brain microvascular endothelial cells, Asiaticoside (50µM; 24h) effectively attenuated Aβ1-42-induced cell growth inhibition [6].
In Swiss mice, Asiaticoside (5-40mg/kg; po; single administration) treatment increased the percentage of mice entering the open arm and the time they stayed in the open arm [7], In Swiss mice Asiaticoside (10-20mg/kg; po; single administration) significantly shortened the immobility time of mice in the tail suspension test [8].
References:
[1]. Shukla A, Rasik A M, Jain G K, et al. In vitro and in vivo wound healing activity of asiaticoside isolated from Centella asiatica[J]. Journal of ethnopharmacology, 1999, 65(1): 1-11.
[2]. Tang B, Zhu B, Liang Y, et al. Asiaticoside suppresses collagen expression and TGF-β/Smad signaling through inducing Smad7 and inhibiting TGF-βRI and TGF-βRII in keloid fibroblasts[J]. Archives of dermatological research, 2011, 303(8): 563-572.
[3]. Lee J, Jung E, Kim Y, et al. Asiaticoside induces human collagen I synthesis through TGFβ receptor I kinase (TβRI kinase)-independent Smad signaling[J]. Planta medica, 2006, 72(04): 324-328.
[4]. Bandopadhyay S, Mandal S, Ghorai M, et al. Therapeutic properties and pharmacological activities of asiaticoside and madecassoside: A review[J]. Journal of cellular and molecular medicine, 2023, 27(5): 593-608.
[5]. Zhou X, Ke C, Lv Y, et al. Asiaticoside suppresses cell proliferation by inhibiting the NF‑κB signaling pathway in colorectal cancer[J]. International Journal of Molecular Medicine, 2020, 46(4): 1525-1537.
[6]. Song D, Jiang X, Liu Y, et al. Asiaticoside attenuates cell growth inhibition and apoptosis induced by Aβ1-42 via inhibiting the TLR4/NF-κB signaling pathway in human brain microvascular endothelial cells[J]. Frontiers in pharmacology, 2018, 9: 28.
[7]. Chen S W, Wang W J, Li W J, et al. Anxiolytic-like effect of asiaticoside in mice[J]. Pharmacology Biochemistry and Behavior, 2006, 85(2): 339-344.
[8]. Liang X, Huang Y N, Chen S W, et al. Antidepressant-like effect of asiaticoside in mice[J]. Pharmacology Biochemistry and Behavior, 2008, 89(3): 444-449.
| Cell experiment [1]: | |
Cell lines | HCT116 cells |
Preparation Method | Cell viability was assessed using a CCK-8 assay. HCT116 cells in the logarithmic growth phase were digested and seeded into 96-well plates at a density of 5×103 cells/well followed by treatment with various concentrations of Asiaticoside (0.1, 0.5 and 2µM) at 37℃. Cells treated with 0.3% DMSO were used as the negative controls. Subsequently, 10% CCK-8 reagent was added to each well at different time intervals (24, 48 and 72h), and cells incubated for 2h at 37℃. The absorbance was then measured using a microplate reader at a wavelength of 450nm. |
Reaction Conditions | 0.1-2µM; 24-72h |
Applications | Asiaticoside significantly reduced cell viability in a time- and dose-dependent manner. |
| Animal experiment [2]: | |
Animal models | Swiss mice |
Preparation Method | The elevated plus-maze consists of four arms (30 × 5cm) elevated 45cm above the floor, with each arm positioned at 90° relative to the adjacent arms. The two enclosed arms had 30cm walls and to facilitate grip on the open arms these included a raised edge of 0.25cm. Open and closed arms were connected via a central area (5 × 5cm) to form a plus sign. The maze floor was constructed of black Plexiglas and the wall of the enclosed arms was constructed of clear Plexiglas. Four 25-W red fluorescent lights arranged as a cross at 100cm above the maze were used as the source of illumination. Mice (n= 9-10 per group) were randomly assigned (with a slight adjustment for matched body-weight) to eight experimental groups (vehicle-control, 0.5, 1, 2mg/kg diazepam or 5, 10, 20, 40mg/kg Asiaticoside). Drug was administration PO 60min prior to the test. Testing commenced by placing a mouse on the central platform of the maze facing an open arm. The number of entries into and the time spent on each of the two types of arms were recorded during the 5-min trial. An arm entry was defined as all four paws having crossed the dividing line between an arm and the central area. The plus-maze was thoroughly cleaned after each trial. |
Dosage form | 5-40mg/kg; po; single administration |
Applications | Asiaticoside treatment increased the percentage of mice entering the open arm and the time they stayed in the open arm. |
References: | |
| Cas No. | 16830-15-2 | SDF | |
| Synonyms | Ba 2742, NSC 36002, NSC 166062 | ||
| Chemical Name | Asiaticoside | ||
| Canonical SMILES | CC1CCC2(CCC3(C(=CCC4C3(CCC5C4(CC(C(C5(C)CO)O)O)C)C)C2C1C)C)C(=O)OC6C(C(C(C(O6)COC7C(C(C(C(O7)CO)OC8C(C(C(C(O8)C)O)O)O)O)O)O)O)O | ||
| Formula | C48H78O19 | M.Wt | 959.12 |
| Solubility | ≥ 44.6mg/mL in DMSO | Storage | Store at 2-8°C, protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 1.0426 mL | 5.2131 mL | 10.4262 mL |
| 5 mM | 208.5 μL | 1.0426 mL | 2.0852 mL |
| 10 mM | 104.3 μL | 521.3 μL | 1.0426 mL |
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
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- Purity: >98.00%
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Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)
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