GLPBio

CNQX

Catalog No.: GC11799Purity:>98.00%Synonyms: 6cyano7Nitroquinoxaline2,3dione, FG 9065
COASDSData Sheet
CNQX is an effective competitive antagonist of AMPA/kainate receptors, with IC50 values of 0.3µM and 1.5µM, respectively[1].

Products are for research use only. Not for human use. We do not sell to patients.

CNQX
Cas No.: 115066-14-3
10mM (in 1mL DMSO)In Stock
$41.00
1
5mgIn Stock
$37.00
1
10mgIn Stock
$52.00
1
25mgIn Stock
$105.00
1
50mgIn Stock
$165.00
1
100mgIn Stock
$300.00
1
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Description

CNQX is an effective competitive antagonist of AMPA/kainate receptors, with IC50 values of 0.3µM and 1.5µM, respectively[1]. CNQX is a non-NMDA receptor antagonist[2].

In vitro, CNQX (1µM) reduces the frequency of light-induced peak potentials in retinal ganglion cells by 28%±22% and also induces voltage- and magnesium-dependent delayed spike firing[2]. CNQX (0-100 mM; 20-24 h) exhibits dose-dependent toxicity in mixed mouse cortical cells, with neuronal damage effects[3]. CNQX (20 µM) reversibly depolarizes the membrane potential of TRN neurons, inducing smaller depolarizations in thalamic relay neurons in VB[4].

In vivo, CNQX (0.5 µg; i.c.v.) can block hippocampal CA3 pyramidal cell death induced by kainic acid in ICR mice, partially attenuate the increase of p-ERK and the decrease of p-CREB, and inhibit the expression of phosphorylated ERK protein[5]. Bilateral infusion of CNQX (0.5 or 1.25µg) into the amygdala or dorsal hippocampus of rats can block the expression of startle reflex[6].

References:
[1]TAGE HONORÉ, STEVE N. DAVIES, JØRGEN DREJER, et al. Quinoxalinediones: Potent Competitive Non-NMDA Glutamate Receptor Antagonists[J]. SCIENCE.1988(241):701-703.
[2] Jeffrey S. Diamond, David R. Copenhagen. The contribution of NMDA and Non-NMDA receptors to the light-evoked input-output characteristics of retinal ganglion cells[J]. Cell Press. October, 1993:725-738.
[3]Uliasz T F , Hewett S J . A microtiter trypan blue absorbance assay for the quantitative determination of excitotoxic neuronal injury in cell culture[J]. Journal of Neuroscience Methods, 2000, 100(1-2):157-163.
[4]Sang Hun Lee,G. Govindaiah,and Charles L. Cox. Selective Excitatory Actions of DNQX and CNQX in Rat Thalamic Neurons[J].Journal of Neurophysiology, 2010(4):103.
[5]Lee J K , Choi S S , Lee H K ,et al.Effects of MK-801 and CNQX on various neurotoxic responses induced by kainic acid in mice.[J]. Molecules & Cells, 2002, 14(3):339-347.
[6]Kim M, Campeau S, Falls W A, et al. Infusion of the non-NMDA receptor antagonist CNQX into the amygdala blocks the expression of fear-potentiated startle. Behav Neural Biol, 1993, 59(1): 5-8.

Protocol

Cell experiment [1]:

Cell lines

Mixed murine cortical cell

Preparation Method

Mixed murine cortical cell cultures were exposed to kainate (0-100 mM) in the absence or presence of increasing concentrations of CNQX. Then 20-24 h later, injury was assessed by spectrophotometric measurement of lactate dehydrogenase (LDH) activity and trypan blue.

Reaction Conditions

0-100 mM; 20-24 h

Applications

There is a time-dependent increase in neuronal cell death as CNQX concentration increases.
Animal experiment [2]:

Animal models

Male ICR mice

Preparation Method

CNQX were prepared in 5 % DMSO as vehicle. The i.c.v. administrations of KA, MK-801, and CNQX were performed following the procedure established by Laursen and Belknap (1986). Briefly, each mouse was injected at bregma with a 50 µl Hamilton microsyringe fitted with a 26-gauge needle that was inserted to a depth of 2.4 mm. The injection volume was 5µl. CNQX (0.5 µg) were injected 10 min prior to KA injection.

Dosage form

0.5 µg; i.c.v.

Applications

CNQX can block kainic acid-induced pyramidal cell death in the CA3 area of the mouse hippocampus, partially attenuate the increase in p-ERK and the decrease in p-CREB, and inhibit the expression of phosphorylated ERK protein.

References:

[1] Uliasz T F , Hewett S J . A microtiter trypan blue absorbance assay for the quantitative determination of excitotoxic neuronal injury in cell culture[J]. Journal of Neuroscience Methods, 2000, 100(1-2):157-163.
[2] Lee J K , Choi S S , Lee H K ,et al.Effects of MK-801 and CNQX on various neurotoxic responses induced by kainic acid in mice.[J]. Molecules & Cells, 2002, 14(3):339-347.

Product Documents

Purity:>98.00%Appearance:A solid
COASDSData Sheet

Chemical Properties

CAS No.
115066-14-3
Synonyms
6cyano7Nitroquinoxaline2,3dione, FG 9065
Chemical Name
7-nitro-2,3-dioxo-1,2,3,4-tetrahydroquinoxaline-6-carbonitrile
SMILES
O=C1NC2=CC([N+]([O-])=O)=C(C#N)C=C2NC1=O
Molecular Formula
C9H4N4O4
Molecular Weight
232.16
Solubility
≥ 23.2mg/mL in DMSO
Storage
Store at -20°C
General tips
Select a suitable solvent according to the product solubility data. Once prepared, aliquot the stock solution to avoid repeated freeze-thaw cycles.
Use stock solutions within 6 months when stored at -80°C or within 1 month when stored at -20°C.
To improve solubility, warm the tube to 37°C and sonicate it for an appropriate period.
Shipping Condition
Evaluation samples are shipped with blue ice. Other available sizes are shipped at room temperature or with blue ice upon request.

CalculatorsMolarity / Dilution / Molecular Weight / Unit Conversion / In Vivo Formulation / Solubility

g/mol

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