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CNQX (Synonyms: 6cyano7Nitroquinoxaline2,3dione, FG 9065)

Catalog No.GC11799

AMPA/kainate receptor antagonist

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CNQX Chemical Structure

Cas No.: 115066-14-3

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10mM (in 1mL DMSO)
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5mg
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10mg
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25mg
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50mg
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100mg
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Sample solution is provided at 25 µL, 10mM.

Product Documents

Quality Control & SDS

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Protocol

Cell experiment:

CNQX is applied by injecting a bolus into the input line of the chamber over 60 s using a motorized syringe pump. In a subpopulation of neurons, CNQX is bath applied for 5 min. Control injections of physiological saline or vehicle (DMSO) does not alter membrane potential/input resistance during voltage recordings[4].

References:

[1]. Blake JF, et al. CNQX blocks acidic amino acid induced depolarizations and synaptic components mediated by non-NMDA receptors in rathippocampal slices. Neurosci Lett. 1988 Jun 29;89(2):182-6.
[2]. Neuman RS, et al. Blockade of excitatory synaptic transmission by 6-cyano-7-nitroquinoxaline-2,3-dione(CNQX) in the hippocampus in vitro. Neurosci Lett. 1988 Sep 23;92(1):64-8.
[3]. Alford S, et al. CNQX and DNQX block non-NMDA synaptic transmission but not NMDA-evoked locomotion in lamprey spinal cord. Brain Res. 1990 Jan 8;506(2):297-302.
[4]. Lee SH, et al. Selective excitatory actions of DNQX and CNQX in rat thalamic neurons. J Neurophysiol. 2010 Apr;103(4):1728-34.
[5]. Kim M, et al. Infusion of the non-NMDA receptor antagonist CNQX into the amygdala blocks the expression of fear-potentiated startle. Behav Neural Biol. 1993 Jan;59(1):5-8.

Background

CNQX (FG9065) is a potent AMPA/kainate receptor antagonist.

In rat hippocampal slices bathed in Mg2+-free medium, 10 μM CNQX reversibly blocks responses to a-amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid (AMPA), quisqualate and kainate but not NMDA[1]. Superfusion of hippocampal slices with 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX, 2-5 μM) reversibly blocks the Schaffer collateral and mossy fibre excitatory postsynaptic potential (EPSP), while sparing the fast and slow GABA-mediated inhibition[2]. CNQX (1-5 μM) produces a selective and dose-dependent reduction in the amplitude of the monosynaptic component of the DR-VRR recorded from lumbar spinal segments[3]. CNQX-mediated depolarizations are mediated by AMPAR but not kainate receptors in TRN neurons[4].

The bilateral infusion of CNQX (0.5 or 1.25 μg) into the amygdala or dorsal hippocampus 10 min prior to a retention test partially blocks the expression of stepdown inhibitory avoidance in rats 24 h after training. CNQX causes a complete blockade at a dose of 0.5 μg[5].

References:
[1]. Blake JF, et al. CNQX blocks acidic amino acid induced depolarizations and synaptic components mediated by non-NMDA receptors in rathippocampal slices. Neurosci Lett. 1988 Jun 29;89(2):182-6.
[2]. Neuman RS, et al. Blockade of excitatory synaptic transmission by 6-cyano-7-nitroquinoxaline-2,3-dione(CNQX) in the hippocampus in vitro. Neurosci Lett. 1988 Sep 23;92(1):64-8.
[3]. Alford S, et al. CNQX and DNQX block non-NMDA synaptic transmission but not NMDA-evoked locomotion in lamprey spinal cord. Brain Res. 1990 Jan 8;506(2):297-302.
[4]. Lee SH, et al. Selective excitatory actions of DNQX and CNQX in rat thalamic neurons. J Neurophysiol. 2010 Apr;103(4):1728-34.
[5]. Kim M, et al. Infusion of the non-NMDA receptor antagonist CNQX into the amygdala blocks the expression of fear-potentiated startle. Behav Neural Biol. 1993 Jan;59(1):5-8.

Chemical Properties

Cas No. 115066-14-3 SDF
Synonyms 6cyano7Nitroquinoxaline2,3dione, FG 9065
Chemical Name 7-nitro-2,3-dioxo-1,2,3,4-tetrahydroquinoxaline-6-carbonitrile
Canonical SMILES O=C1NC2=CC([N+]([O-])=O)=C(C#N)C=C2NC1=O
Formula C9H4N4O4 M.Wt 232.16
Solubility ≥ 23.2mg/mL in DMSO Storage Store at -20°C
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table

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1 mg 5 mg 10 mg
1 mM 4.3074 mL 21.5369 mL 43.0737 mL
5 mM 0.8615 mL 4.3074 mL 8.6147 mL
10 mM 0.4307 mL 2.1537 mL 4.3074 mL
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Average Rating: 5 ★★★★★ (Based on Reviews and 9 reference(s) in Google Scholar.)

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