Riboflavin phosphate |
Catalog No.GC37530
One-Click Copy Product Info
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Riboflavin phosphate sodium is a water-soluble phosphate of riboflavin, which retains the main structure and function of riboflavin.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 130-40-5
Sample solution is provided at 25 µL, 10mM.
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Riboflavin phosphate sodium is a water-soluble phosphate of riboflavin, which retains the main structure and function of riboflavin. the pure Riboflavin phosphate sodium solution exhibits the maximum emission peak at 565nm[1]. Riboflavin phosphate sodium is a well-known oral nutritional supplement, has been widely used to treat localized inflammatory diseases, such as angulus infectiosus, chilitis, glossitis and radiotherapy related esophagitis[2][3].
In vitro, the macrophages were treated with various concentrations of Riboflavin phosphate sodium (6.25-200µg/ml) and were incubated for 24h at 37℃ in slide chambers. Phagocytic activity was then determined. Riboflavin phosphate sodium directly activated macrophages in vitro[4].
In vivo, Riboflavin phosphate sodium (100mg/kg) was injected intraperitoneally or intravenously into ICR male mice at 1 day before the phagocytosis assay. The treated mice showed a significantly higher phagocytic index. Riboflavin phosphate sodium could enhance macrophage function in vivo[4]. Riboflavin phosphate sodium can be applied as the treatment of sepsis and septic shock. Riboflavin phosphate sodium (10, 20, 40, 80mg/kg) was administered into ICR mice 6h after lipopolysaccharide injection or 1h after bacterial infection by 6h i.v. infusion. Riboflavin phosphate sodium significantly improved lipopolysaccharide-induced endotoxin shock and reduced the lethality of Escherichia coli and Staphylococcus aureus infection. The i.v. infusion of Riboflavin phosphate sodium reduced the elevations of proinflammatory cytokines and nitric oxide induced by lipopolysaccharide[5].
References:
[1] Feng G Y, Zhang S T, Zhong S L, et al. Temperature and pH dual-responsive supramolecular hydrogels based on riboflavin sodium phosphate and 2,6-Diaminopurine with thixotropic and fluorescent properties. Colloids and Surfaces A: Physicochemical and Engineering Aspects. 2021 Dec. Volume 630, 127548
[2] Kodama K, Suzuki M, Toyosawa T, Araki S. Inhibitory mechanisms of highly purified vitamin B2 on the productions of proinflammatory cytokine and NO in endotoxin-induced shock in mice. Life Sci. 2005 Nov 26;78(2):134-9.
[3] Shen K, Huang X E. Clinical investigation in effect of riboflavin sodium phosphate on prevention and treatment for patients with radiotherapy related esophagitis. Asian Pac J Cancer Prev. 2015;16(4):1525-7.
[4] Kimura, M., Suzuki, M., Araki, S., et al. In vitro and in vivo effects of riboflavin sodium phosphate on the phagocytic activity of peritoneal macrophages in mice. Animal Science and Technology. 1996. 67, 368 – 373.
[5] Toyosawa T, Suzuki M, Kodama K, Araki S. Effects of intravenous infusion of highly purified vitamin B2 on lipopolysaccharide-induced shock and bacterial infection in mice. Eur J Pharmacol. 2004 May 25;492(2-3):273-80.
| Cell experiment [1]: | |
Cell lines | Mouse macrophages |
Preparation Method | Macrophages were harvested from 10 untreated mice and pooled. Then the macrophages were treated with various concentrations of Riboflavin phosphate sodium or 100μg/ml muramyldipeptide, and were incubated for 24h at 37℃ in slide chambers. |
Reaction Conditions | 6.25, 12.5, 25, 50, 100, 200μg/mL; 24h |
Applications | The phagocytosis of latex particles by mouse peritoneal macrophages showed that Riboflavin phosphate sodium directly activated macrophages in vitro. |
| Animal experiment [2]: | |
Animal models | Male ICR mice |
Preparation Method | Mice were given an i.v. bolus injection of lipopolysaccharide at 12mg/kg through the tail vein. Six hours after lipopolysaccharide injection, mice were treated with saline (control), Riboflavin phosphate sodium (10, 20, 40 and 80mg/kg) by i.v. infusion for 6h at a speed of 0.58ml/h via the tail vein using an infusion pump. |
Dosage form | 10, 20, 40 and 80mg/kg; i.v.; 6h |
Applications | Riboflavin phosphate sodium significantly reduced the lethality of Escherichia coli and Staphylococcus aureus infection. The i.v. infusion of Riboflavin phosphate sodium reduced the elevations of proinflammatory cytokines and nitric oxide induced by lipopolysaccharide. |
References: | |
| Cas No. | 130-40-5 | SDF | |
| Canonical SMILES | CC1=CC2=C(C=C1C)N=C(C(N2C[C@H](O)[C@H](O)[C@H](O)COP([O-])(O)=O)=N3)C(NC3=O)=O.[Na+] | ||
| Formula | C17H20N4NaO9P | M.Wt | 478.33 |
| Solubility | 50mg/mL in Water | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.0906 mL | 10.453 mL | 20.9061 mL |
| 5 mM | 418.1 μL | 2.0906 mL | 4.1812 mL |
| 10 mM | 209.1 μL | 1.0453 mL | 2.0906 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >73.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 33 reference(s) in Google Scholar.)