VIP(6-28)(human, rat, porcine, bovine) is a potent vasoactive intestinal peptide (VIP) antagonist. In neuron-rich culture systems, VIP(6-28)(human, rat, porcine, bovine) inhibits VIP-induced increases in cAMP and VIP-PHI mRNA[1].
VIP(6-28)(human, rat, porcine, bovine) (100μM; 48h) inhibited the VIP‑stimulated increase in VIP–PHI mRNA by 60%, while having no effect on basal VIP–PHI mRNA levels on its own in neonatal dissociated rat SCGs[1]. In short‑term cultures of adult rat SCGs, VIP(6-28)(human, rat, porcine, bovine) at concentrations of 10, 30, or 100μM for 1h inhibited the 10μM VIP‑induced elevation of cAMP levels by 52%, 64%, and 81%, respectively[1]. In human odontoblast-like cells, VIP(6-28)(human, rat, porcine, bovine) reduced the VIP-induced cAMP elevation to 66.4% and 55.8% of control at 10nM and 100nM for 2min, respectively[2]. VIP(6-28)(human, rat, porcine, bovine) (5µM; 48h) antagonized the VIP-induced promotion of follicle activation in rat ovarian follicles[3].
References:
[1] Mohney RP, Zigmond RE. Vasoactive intestinal peptide enhances its own expression in sympathetic neurons after injury. J Neurosci. 1998;18(14):5285-5293.
[2] Kitayama E, Kimura M, Ouchi T, Furusawa M, Shibukawa Y. Functional Expression of IP, 5-HT4, D1, A2A, and VIP Receptors in Human Odontoblast Cell Line. Biomolecules. 2023;13(6):879.
[3] Li S, Fan Q, Xie Y, et al. VIP activates primordial follicles of rat through ERK-mTOR pathway in tissue culture. Reproduction. 2019;157(5):475-484.