5-Fluorouridine |
Catalog No.GC38141
One-Click Copy Product Info
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5-Fluorouridine is a ribonucleotide metabolite of 5-Fluorouracil with anticancer activity. 5-Fluorouridine can mark active transcription sites of cells in vivo and in vitro for immunodetection of nascent RNA.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 316-46-1
Sample solution is provided at 25 µL, 10mM.
5-Fluorouridine is a ribonucleotide metabolite of 5-Fluorouracil with anticancer activity[1]. 5-Fluorouridine can mark active transcription sites of cells in vivo and in vitro for immunodetection of nascent RNA[2, 3]. 5-Fluorouridine is an organofluorine compound belonging to pyrimidine nucleosides and their analogs. It is uridine with a fluorine substituent at the 5th position of the uracil ring and has a mutagenic effect[4].
In vitro, 5-Fluorouridine (10µM) treatment of HCT-116 cells for 24h increased the percentage of apoptotic cells, affected the expression of 1200 different genes during apoptosis, and increased the expression of macrophage inhibitory cytokine 1 (MIC-1) by up to 100-fold[5]. 5-Fluorouridine (167μM) treatment of gastric cancer cells (MKN45 and MKN28 cells) for 24-96h inhibited cell proliferation in a time-dependent manner[6].
In vivo, 5-Fluorouridine (567-1500mg/kg/day) was intraperitoneally injected into male CBA/J mice for 20 days, which induced gastrointestinal toxicity in mice[7].
References:
[1]Ghoshal K, Jacob S T. An alternative molecular mechanism of action of 5-fluorouracil, a potent anticancer drug[J]. Biochemical pharmacology, 1997, 53(11): 1569-1575.
[2]Puente-Bedia A, Berciano M T, Tapia O, et al. Nuclear reorganization in hippocampal granule cell neurons from a mouse model of Down syndrome: changes in chromatin configuration, nucleoli and Cajal bodies[J]. International Journal of Molecular Sciences, 2021, 22(3): 1259.
[3]Awasthi S, Verma M, Mahesh A, et al. DDX49 is an RNA helicase that affects translation by regulating mRNA export and the levels of pre-ribosomal RNA[J]. Nucleic Acids Research, 2018, 46(12): 6304-6317.
[4]Gmeiner W H. Chemistry of fluorinated pyrimidines in the era of personalized medicine[J]. Molecules, 2020, 25(15): 3438.
[5]Schmittgen T D, Gissel K A, Zakrajsek B A, et al. Diverse gene expression pattern during 5-fluorouridine-induced apoptosis[J]. International journal of oncology, 2005, 27(2): 297-306.
[6]Wu F, Li R T, Yang M, et al. Gelatinases-stimuli nanoparticles encapsulating 5-fluorouridine and 5-aza-2′-deoxycytidine enhance the sensitivity of gastric cancer cells to chemical therapeutics[J]. Cancer Letters, 2015, 363(1): 7-16.
[7]Houghton J A, Houghton P J, Wooten R S. Mechanism of induction of gastrointestinal toxicity in the mouse by 5-fluorouracil, 5-fluorouridine, and 5-fluoro-2′-deoxyuridine[J]. Cancer research, 1979, 39(7_Part_1): 2406-2413.
This plan only provides a guide, please modify it to meet your specific needs.
1. Solution preparation
(1) Working solution: Dissolve 5-Fluorouridine in the experimental buffer and prepare the working solution of the required concentration.
Note: Please adjust the optimal working concentration according to the actual situation or refer to the literature to set the gradient concentration by yourself. The working solution must be prepared and used immediately.
2. 5-Fluorouridine in vivo labeling[1](Source literature, for reference only)
(1) Inject 5-Fluorouridine intraperitoneally into mice at a dose of 10μL/g. 0.4M 5-Fluorouridine stock solution was dissolved in 0.9% saline.
(2) Euthanize the mice 45min after injection and fix them by perfusion with 3.7% paraformaldehyde in HPEM buffer (2×HPEM: 60mM Hepes; 130mM Pipes; 20mM EGTA; 4mM MgCl2·6H2O) containing 0.5% Triton X for 10min.
(3) The brain was removed, fixed with perfusion buffer for 1h, and washed in HPEM.
(4) Mechanical hippocampal granule cell dissociation was performed.
(5) The cells were treated with mouse monoclonal anti-BrdU at 37°C for 1h.
(6) After washing with PBS solution containing 0.1% Tween20, the cells were incubated with FITC-conjugated secondary antibody for 45min at room temperature.
(7) After washing with PBS, the slides were sealed and the incorporation of 5-Fluorouridine into RNA was detected under a fluorescence microscope.
3. 5-Fluorouridine labeling in vitro[2] (Source literature, for reference only)
(1) The cells were pulse-labeled with 2mM 5-Fluorouridine for 20min.
(2) The cells were fixed with 2% formaldehyde for 10min, permeabilized with 0.5% Triton X-100 for 10min, and then blocked with PBS solution containing 0.1% Tween20 for 1h.
(3) Incubate with BrdU antibody at 4°C overnight.
(4) After washing with PBS, incubate the cells with FITC-conjugated secondary antibody at room temperature for 1h.
(5) After washing with PBS, treat the cells with DAPI and detect the incorporation of 5-Fluorouridine into RNA under a fluorescence microscope.
Note: For your safety and health, please wear a lab coat and disposable gloves when operating.
References:
[1] Puente-Bedia A, Berciano M T, Tapia O, et al. Nuclear reorganization in hippocampal granule cell neurons from a mouse model of Down syndrome: changes in chromatin configuration, nucleoli and Cajal bodies[J]. International Journal of Molecular Sciences, 2021, 22(3): 1259.
[2]Awasthi S, Verma M, Mahesh A, et al. DDX49 is an RNA helicase that affects translation by regulating mRNA export and the levels of pre-ribosomal RNA[J]. Nucleic Acids Research, 2018, 46(12): 6304-6317.
| Cas No. | 316-46-1 | SDF | |
| Canonical SMILES | OC[C@@H]1[C@H]([C@H]([C@H](N2C(NC(C(F)=C2)=O)=O)O1)O)O | ||
| Formula | C9H11FN2O6 | M.Wt | 262.19 |
| Solubility | Water: 100 mg/mL (381.40 mM); DMSO: ≥ 100 mg/mL (381.40 mM) | Storage | Store at 4°C, stored under nitrogen |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.814 mL | 19.0701 mL | 38.1403 mL |
| 5 mM | 762.8 μL | 3.814 mL | 7.6281 mL |
| 10 mM | 381.4 μL | 1.907 mL | 3.814 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 8 reference(s) in Google Scholar.)