Dehydrodiisoeugenol
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| Pack Size | Price | Availability | Quantity | Action |
|---|---|---|---|---|
| 10mM (in 1mL DMSO) | $70.00 | In Stock | 1 | |
| 1mg | $24.00 | In Stock | 1 | |
| 5mg | $64.00 | In Stock | 1 | |
| 10mg | $112.00 | In Stock | 1 | |
| 20mg | $184.00 | In Stock | 1 |
Citations
1 publicationsPublications from researchers using this productDescription
Dehydrodiisoeugenol (DEH) is a representative and major benzofuran-type neolignan found in Myristica fragrans Houtt, possessing antibacterial, anti-inflammatory, anticancer, and antioxidant properties. Dehydrodiisoeugenol inhibits the proliferation of colorectal cancer cells and induces apoptosis, autophagy, endoplasmic reticulum stress and cell cycle arrest. Dehydrodiisoeugenol is commonly used in the research related to colorectal cancer, inflammatory diseases and ulcerative colitis[1][2][3].
In vitro, treatment with Dehydrodiisoeugenol (2.5, 5 and 10µM) for 24h inhibited the activation of the NF-κB and MAPK signaling pathways and dose-dependently suppressed the expression of inflammatory mediators, including NO, iNOS, COX-2, PGE₂, IL-1β, IL-6, and TNF-α in the LPS/IFNγ-induced RAW264.7 ulcerative colitis (UC) model[1]. Treatment with Dehydrodiisoeugenol (40, 80, and 100µM) for 24h significantly inhibited the viability of HCT116 and HT29 cells[1]. Treatment with Dehydrodiisoeugenol at 40µM for 24h induced apoptosis in HCT116 cells, suppressing Bcl-2 expression while upregulating Bax expression[1].
In vivo, oral administration of Dehydrodiisoeugenol at 40mg/kg for 20 days significantly inhibited tumor growth in BALB/c nude mice transplanted with HCT116 cells[1]. Oral gavage administration of Dehydrodiisoeugenol at 20mg/kg/d for 8 days significantly improved rectal bleeding in DSS-induced mice[1].
References:
[1] Yi F, Chen N, Zhao M, et al. Dehydrodiisoeugenol targets NOD2 exerting dual effects against colitis and colorectal cancer: a double-edged sword. Mol Med. 2025;31(1):221.
[2] Li C, Zhang K, Pan G, et al. Dehydrodiisoeugenol inhibits colorectal cancer growth by endoplasmic reticulum stress-induced autophagic pathways. J Exp Clin Cancer Res. 2021;40(1):125.
[3] Murakami Y, Shoji M, Hirata A, Tanaka S, Yokoe I, Fujisawa S. Dehydrodiisoeugenol, an isoeugenol dimer, inhibits lipopolysaccharide-stimulated nuclear factor kappa B activation and cyclooxygenase-2 expression in macrophages. Arch Biochem Biophys. 2005;434(2):326-332.
Protocol
| Cell experiment [1]: | |
Cell lines | RAW264.7 |
Preparation Method | RAW 264.7 cells were seeded overnight in a 96-well plate, and treated with the positive control drug (curcumin, 5µM) and Dehydrodiisoeugenol (2.5, 5, 10µM), following stimulated by LPS (0.5µg/ml) and recombinant IFNγ (10ng/ml) for 24h, determining the concentration of various inflammatory factors in cell culture supernatant. |
Reaction Conditions | 2.5, 5, 10µM; 24h |
Applications | Dehydrodiisoeugenol could inhibit the production of NO, iNOS, COX2, PGE2, IL-1β, IL-6, and TNFα in LPS/IFNγ-stimulated RAW264.7 cells in a dose-dependent manner. |
| Animal experiment [1]: | |
Animal models | BALB/c nude mice transplanted with HCT116 cells |
Preparation Method | BALB/c nude mice were subcutaneously injected with HCT116 cells into their bodies. About one week later, the successfully transplanted mice were randomly divided into the control group and the Dehydrodiisoeugenol (DEH) group. Then, the control group received intraperitoneal injection of 100µL PBS (containing 0.1% DMSO), while the mice in the DEH group were orally gavaged with DEH at 40mg/kg once daily for 20 consecutive days. Tumor sizes and body weights were measured and recorded every 5 days. The mice were euthanized, and tumors were excised, photographed, and weighed. |
Dosage form | 40mg/kg; 20d; p.o. |
Applications | There was no significant difference in average weight between the two groups of mice. However, tumor weight and volume were significantly smaller in the DEH (40mg/kg) treated group compared to the control group. |
References: | |
Product Documents
Chemical Properties
Use stock solutions within 6 months when stored at -80°C or within 1 month when stored at -20°C.
To improve solubility, warm the tube to 37°C and sonicate it for an appropriate period.
CalculatorsMolarity / Dilution / Molecular Weight / Unit Conversion / In Vivo Formulation / Solubility
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