β-Amyrin (Synonyms: (+)-β-Amyrin, NSC 527971, Olean-12-en3β-ol) |
Catalog No.GC45603
One-Click Copy Product Info
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β-Amyrin is a naturally occurring pentacyclic triterpenoid compound that possesses anti-inflammatory, antibacterial, and antioxidant activities.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 559-70-6
Sample solution is provided at 25 µL, 10mM.
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β-Amyrin is a naturally occurring pentacyclic triterpenoid compound that possesses anti-inflammatory, antibacterial, and antioxidant activities[1-2]. β-Amyrin can be used in research related to Alzheimer's disease, cancer, obesity, atherosclerosis, and type II diabetes[3-4].
In vitro, Hep-G2 hepatocellular carcinoma cells were treated with β-Amyrin (0-100μM) for 24 hours. β-Amyrin significantly induced apoptosis and caused cell cycle arrest at the G2/M phase, while simultaneously activating the JNK and p38 signaling pathways[5]. Escherichia coli (E. coli) cells were treated with β-Amyrin (5μM) for 2 hours. β-Amyrin induced apoptosis-like death in the cells by triggering the accumulation of reactive oxygen species (ROS) and glutathione dysfunction, which led to cell membrane depolarization, resulting in DNA fragmentation and phosphatidylserine externalization[6].
In vivo, an amyloid-β (Aβ)-injected Alzheimer's disease (AD) mouse model was orally administered β-Amyrin (4mg/kg) daily from day 1 to day 5 after Aβ (0.5mg; 35-40μl; or 1mg; 70-80μl) injection. β-Amyrin significantly ameliorated the Aβ-induced impairments in object recognition memory and passive avoidance memory[7]. ICR mice with scopolamine-induced memory impairment were orally administered β-Amyrin (4mg/kg) 60 minutes before training. β-Amyrin significantly ameliorated the scopolamine-induced memory impairment in the passive avoidance task[8].
References:
[1] Viet TD, Xuan TD, Anh H. α-Amyrin and β-Amyrin Isolated from Celastrus hindsii Leaves and Their Antioxidant, Anti-Xanthine Oxidase, and Anti-Tyrosinase Potentials. Molecules. 2021 Nov 29;26(23):7248.
[2] Kwun MS, Lee HJ, Lee DG. β-amyrin-induced apoptosis in Candida albicans triggered by calcium. Fungal Biol. 2021 Aug;125(8):630-636.
[3] Oliveira RC, Bandeira PN, Lemos TLG, et al. In silico and in vitro evaluation of efflux pumps inhibition of α,β-amyrin. J Biomol Struct Dyn. 2022;40(23):12785-12799.
[4] Mus AA, Goh LPW, Marbawi H, et al. The Biosynthesis and Medicinal Properties of Taraxerol. Biomedicines. 2022 Mar 30;10(4):807.
[5] Wen S, Gu D, Zeng H. Antitumor effects of beta-amyrin in Hep-G2 liver carcinoma cells are mediated via apoptosis induction, cell cycle disruption and activation of JNK and P38 signalling pathways. J BUON. 2018 Jul-Aug;23(4):965-970.
[6] Han G, Lee DG. Antibacterial Mode of Action of β-Amyrin Promotes Apoptosis-Like Death in Escherichia coli by Producing Reactive Oxygen Species. J Microbiol Biotechnol. 2022 Dec 28;32(12):1547-1552.
[7] Park HJ, Kwon H, Lee JH, et al. β-Amyrin Ameliorates Alzheimer's Disease-Like Aberrant Synaptic Plasticity in the Mouse Hippocampus. Biomol Ther (Seoul). 2020 Jan 1;28(1):74-82.
[8] Park SJ, Ahn YJ, Oh SR, et al. Amyrin attenuates scopolamine-induced cognitive impairment in mice. Biol Pharm Bull. 2014;37(7):1207-13.
| Cell experiment [1]: | |
Cell lines | Hep-G2 cells (human liver carcinoma cell line) |
Preparation Method | Hep-G2 cells were maintained in RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS) at 37°C, 5% CO₂. Hep-G2 cells were treated with β-Amyrin (0-100μM) for 24 hours. |
Reaction Conditions | 0-100μM; 24h. |
Applications | β-Amyrin significantly induced apoptosis in Hep-G2 cells. β-Amyrin also caused cell cycle arrest at the G2/M phase. β-Amyrin activated the JNK and p38 signaling pathways. |
| Animal experiment [2]: | |
Animal models | ICR mice |
Preparation Method | Mice were orally administered a single dose of β-Amyrin (4mg/kg) 60 minutes before the acquisition trial of a step-through passive avoidance task. Memory impairment was induced by an intraperitoneal (i.p.) injection of scopolamine (1mg/kg) 30 minutes before the acquisition trial. A retention trial was conducted 24 hours later. |
Dosage form | 4mg/kg; p.o.; single administration. |
Applications | A single administration of β-Amyrin (4mg/kg) significantly ameliorated the scopolamine-induced deficits in contextual long-term memory in the passive avoidance task. It also enhanced the phosphorylation levels of extracellular signal-regulated kinase (ERK) and glycogen synthase kinase-3β (GSK-3β) in the hippocampal tissue. |
References: | |
| Cas No. | 559-70-6 | SDF | |
| Synonyms | (+)-β-Amyrin, NSC 527971, Olean-12-en3β-ol | ||
| Canonical SMILES | CC1(C)[C@]2([H])CC[C@@]3(C)[C@]4(C)CC[C@@]5(C)CCC(C)(C)C[C@@]5([H])C4=CC[C@]3([H])[C@@]2(C)CC[C@@H]1O | ||
| Formula | C30H50O | M.Wt | 426.7 |
| Solubility | DMF: 3 mg/ ml,DMF:PBS (pH 7.2) (1:8): 0.11 mg/ml,Ethanol: 1 mg/ml,Soluble in Chloroform | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.3436 mL | 11.7178 mL | 23.4357 mL |
| 5 mM | 468.7 μL | 2.3436 mL | 4.6871 mL |
| 10 mM | 234.4 μL | 1.1718 mL | 2.3436 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
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- Purity: >95.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 28 reference(s) in Google Scholar.)