Home>>Signaling Pathways>> Proteases>> Endogenous Metabolite>>Heparan Sulfate
Heparan Sulfate Catalog No.GC30767

Size Price Stock Qty
1mg
$101.00
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5mg
$405.00
In stock
10mg
$708.00
In stock
25mg
$1,288.00
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Sample solution is provided at 25 µL, 10mM.

Quality Control

Quality Control & SDS

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Protocol

Animal experiment:

Rats: Sprague-Dawley male rats 3-4 months old receive aspiration of a small segment of the motor-sensory cortex in both hemispheres. Groups of rats (n=5) receive a piece of Gelfoam embedded with human recombinant FGF-2, heparan sulfate (10 kg/mL; sodium salt, bovine kidney) dissolved in PBS, or both, in the right hemisphere. The wound cavity of the left hemisphere receives a piece of Gelfoam embedded with saline[5].

References:

[1]. Kraushaar DC, et al. Heparan sulfate: a key regulator of embryonic stem cell fate. Biol Chem. 2013 Jun;394(6):741-51.
[2]. Sarrazin S, et al. Heparan sulfate proteoglycans. Cold Spring Harb Perspect Biol. 2011 Jul 1;3(7). pii: a004952.
[3]. Yamamoto S, et al. Heparan sulfate on intestinal epithelial cells plays a critical role in intestinal crypt homeostasis via Wnt/β-catenin signaling. Am J Physiol Gastrointest Liver Physiol. 2013 Aug 1;305(3):G241-9.
[4]. Minge D, et al. Heparan Sulfates Support Pyramidal Cell Excitability, Synaptic Plasticity, and Context Discrimination. Cereb Cortex. 2017 Feb 1;27(2):903-918.
[5]. Gómez-Pinilla F, et al. Regulation of astrocyte proliferation by FGF-2 and heparan sulfate in vivo. J Neurosci. 1995 Mar;15(3 Pt 1):2021-9.

Chemical Properties

Cas No. 9050-30-0 SDF Download SDF
Synonyms N/A
Chemical Name N/A
Canonical SMILES O=S(OCC1O[C@@H](OC)[C@@H](NS(=O)([O-])=O)[C@@H](OS(=O)([O-])=O)[C@H]1O[C@@H]2OC(C([O-])=O)[C@H](O[C@@H]3OC(COS(=O)([O-])=O)[C@H](O[C@@H]4OC(C([O-])=O)[C@H](OC)[C@H](O)[C@@H]4OS(=O)([O-])=O)[C@H](O)[C@@H]3NS(=O)([O-])=O)[C@H](O)[C@@H]2O)([O-])=O.[n]
Formula C12H19NO20S3 (monomer) M.Wt 593.47(monomer)
Solubility H2O : 47.1 mg/mL (Need ultrasonic and warming) Storage Store at -20°C
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.
Shipping Condition Evaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request
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Background

Heparan sulfate, a complex and linear polysaccharide, exists as part of glycoproteins named heparan sulfate proteoglycans, which are expressed abundantly on the cell surface and in the extracellular matrix.

Heparan sulfate is a linear polysaccharide and belongs to the family of glycosaminoglycans. Heparan sulfate is composed of glucuronic acid (GlcA) and iduronic acid (IdoA) residues as well as N-acetyl glucosamines (GlcNAc) with various sulfation modifications, and is typically 50-200 disaccharides in length. Heparan sulfate interacts with numerous proteins, including growth factors, morphogens, and adhesion molecules, and thereby regulates important developmental processes in invertebrates and vertebrates. Heparan sulfate chains regulate developmental signaling by acting as co-factors through a variety of mechanisms that include but are not limited to maintenance of morphogen gradients and co-receptor functions[1]. Heparan sulfate proteoglycans can act as receptors for proteases and protease inhibitors regulating their spatial distribution and activity. Membrane Heparan sulfate proteoglycans act as coreceptors for various tyrosine kinase-type growth factor receptors, lowering their activation threshold or changing the duration of signaling reactions[2]. Heparan sulfate influences the binding affinity of intestinal epithelium cells to Wnt, thereby promoting activation of canonical Wnt signaling and facilitating regeneration of small intestinal crypts after epithelial injury[3].

Digestion of heparan sulfate impairs context discrimination in a fear conditioning paradigm and oscillatory network activity in the low theta band after fear conditioning. Thus, heparan sulfate maintains neuronal excitability and, as a consequence, support synaptic plasticity and learning[4]. FGF-2/FGFR system is involved in the regulation of astrocytic reactivity and/or proliferation in the brain and its action is potentiated by heparan sulfate[5].