Hinokitiol (Synonyms: NSC 18804, β-Thujaplicin, 4-isopropyl Tropolone) |
Catalog No.GC11302
One-Click Copy Product Info
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Hinokitiol is a naturally occurring tropolone compound from the Formosan cypress with antibacterial and anticancer properties.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 499-44-5
Sample solution is provided at 25 µL, 10mM.
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Hinokitiol is a naturally occurring tropolone compound from the Formosan cypress with antibacterial and anticancer properties [1]. Hinokitiol induces oxidative stress, cytotoxicity, and autophagy through modulation of PI3K/Akt/mTOR, p38/ERK/MAPK, nuclear factor kappa B, and c-Jun N-terminal kinase signaling pathways [2]. Hinokitiol has been widely used to inhibit the metabolic processes of bacterial cell membranes, regulate respiration and membrane permeability, thereby effectively killing various bacteria[3].
In vitro, Hinokitiol treatment for 48 hours significantly reduced the cell viability of Ishikawa cells, HEC-1A cells, and KLE cells, with IC50 values of 13.33μM, 49.51μM, and 4.69μM, respectively[4]. Treatment with 30μg/ml Hinokitiol for 24 hours led to the loss of cell membrane integrity and cell cycle arrest in KB-1 cells[5]. Treatment with 40µM Hinokitiol for 24 hours blocked the process of HeLa cells transitioning from the G2/M phase to the G1 phase, increased the expression levels of p21 and p53, promoted cytoplasmic expansion and enhanced SA-β-gal activity, and induced cell senescence[6].
In vivo, Hinokitiol treatment via oral administration at a dose of 100mg/kg every other day for 3 weeks significantly reduced tumor volume and tumor weight in the HCT-116 xenograft mouse model[7]. A single intravenous injection of 1.7mg/kg dose of Hinokitiol can alleviate liver injury induced by hemorrhagic shock/resuscitation in mice within 24 hours[8].
References:
[1] El Hachlafi N, Lakhdar F, Khouchlaa A, et al. Health benefits and pharmacological properties of hinokitiol[J]. Processes, 2021, 9(9): 1680.
[2] Bhuia M S, Chowdhury R, Afroz M, et al. Therapeutic efficacy studies on the Monoterpenoid Hinokitiol in the treatment of different types of cancer[J]. Chemistry & biodiversity, 2025, 22(5): e202401904.
[3] Morita Y, Sakagami Y, Okabe T, et al. The mechanism of the bactericidal activity of hinokitiol[J]. Biocontrol Science, 2007, 12(3): 101-110.
[4] Chen H Y, Cheng W P, Chiang Y F, et al. Hinokitiol exhibits antitumor properties through induction of ROS-mediated apoptosis and p53-driven cell-cycle arrest in endometrial cancer cell lines (Ishikawa, HEC-1A, KLE)[J]. International journal of molecular sciences, 2021, 22(15): 8268.
[5] Roy A, Cheriyan B V, Perumal E, et al. Effect of hinokitiol in ameliorating oral cancer: in vitro and in silico evidences[J]. Odontology, 2025, 113(2): 750-763.
[6] Wang C C, Chen B K, Chen P H, et al. Hinokitiol induces cell death and inhibits epidermal growth factor-induced cell migration and signaling pathways in human cervical adenocarcinoma[J]. Taiwanese Journal of Obstetrics and Gynecology, 2020, 59(5): 698-705.
[7] Lee Y S, Choi K M, Kim W, et al. Hinokitiol inhibits cell growth through induction of S-phase arrest and apoptosis in human colon cancer cells and suppresses tumor growth in a mouse xenograft experiment[J]. Journal of natural products, 2013, 76(12): 2195-2202.
[8] Lu W J, Lin K H, Tseng M F, et al. New therapeutic strategy of hinokitiol in haemorrhagic shock‐induced liver injury[J]. Journal of Cellular and Molecular Medicine, 2019, 23(3): 1723-1734.
| Cell experiment [1]: | |
Cell lines | KLE cells |
Preparation Method | KLE cells were seeded in 96-well plates at a density of 3×103 cells in DMEM/Ham’s F-12 with 10% (v/v) fetal bovine serum (FBS), 100U/ml penicillin, and 100µg/ml streptomycin in a humidified atmosphere containing 5% CO2 for 24h. After 48h of treatment with different concentrations of Hinokitiol (1, 5, 10, 25, and 50µM), the cell viability was measured. |
Reaction Conditions | 1, 5, 10, 25, and 50µM; 48h |
Applications | Hinokitiol treatment significantly inhibited the cell viability of KLE cells in a dose-dependent manner. |
| Animal experiment [2]: | |
Animal models | Male BALB/c-nude mice |
Preparation Method | Male BALB/c-nude mice (6 weeks old; 22-25g) were housed in a room with controlled temperature (21-23°C), humidity (55-60%), and lighting (12h light/dark cycle) and were supplied with water. After being acclimated for 1 week, nude mice were randomly distributed in 3 groups: normal (n=10), HCT-116 xenograft (n=10), HCT-116 xenograft-Hinokitiol treatment (n=10). The mice were inoculated with HCT-116 cells by intradermal injection (2×106 tumor cells in 0.2ml of PBS/mouse) with a 26-gauge needle into the flank. After 2 days, mice were orally administered Hinokitiol (100mg/kg; every other day for 3 weeks). Tumor volumes were periodically measured. |
Dosage form | 100mg/kg; every other day for 3 weeks; p.o. |
Applications | Hinokitiol treatment significantly decreased the tumor volumes in mice with HCT-116 xenografts. |
References: | |
| Cas No. | 499-44-5 | SDF | |
| Synonyms | NSC 18804, β-Thujaplicin, 4-isopropyl Tropolone | ||
| Chemical Name | 2-hydroxy-4-isopropylcyclohepta-2,4,6-trienone | ||
| Canonical SMILES | OC1=CC(C(C)C)=CC=CC1=O | ||
| Formula | C10H12O2 | M.Wt | 164.2 |
| Solubility | ≥ 16.4mg/mL in DMSO | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 6.0901 mL | 30.4507 mL | 60.9013 mL |
| 5 mM | 1.218 mL | 6.0901 mL | 12.1803 mL |
| 10 mM | 609 μL | 3.0451 mL | 6.0901 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 25 reference(s) in Google Scholar.)