JC-1 (Synonyms: CBIC2;JC1;JC 1) |
Catalog No.GC12331 |
JC-1(CBIC2) is a cationic carbonyl cyanine fluorescent dye.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 3520-43-2
Sample solution is provided at 25 µL, 10mM.
JC-1(CBIC2) is a cationic carbonyl cyanine fluorescent dye.JC-1 serves as an excellent fluorescent probe widely employed for the detection of mitochondrial membrane potential. It selectively enters the mitochondria and changes its fluorescence properties with changes in mitochondrial transmembrane potential (ΔΨ m) allowing for the assessment of membrane potential in cells, tissues, or purified mitochondria. In healthy mitochondria, JC-1 forms aggregates within the mitochondrial matrix, giving rise to intense red fluorescence (Ex=585 nm, Em=590 nm).Conversely, under conditions of low mitochondrial membrane potential, JC-1 fails to aggregate in the matrix, resulting in the emission of green fluorescence (Ex=514 nm, Em=529 nm) [1-4].
JC-1, a fluorescent molecule, is also a probe of P-glycoprotein (Pgp). A specific property of JC-1, due to its stacking in a liquid crystal form, is the dependence of its fluorescence emission wavelength on its concentration. On excitation at 490 nm, JC-1 monomers display a cytoplasmic fluorescence emission centered at 537 nm (green band).Beyond a critical concentration, JC-1 aggregates in mitochondrium, thus leading to the emergence of an intense emission band centered at 597 nm (red band), in addition to the cytoplasmic green band. We have shown in cell lines that sensitive cells display both green and red fluorescence. In resistant cells, when Pgp activity increased, green fluorescence of JC-1 decreased and red fluorescence was lost. In intermediate-resistant cells, green fluorescence intensity was often identical to the fluorescence intensity of sensitive cells, but red fluorescence was already lost [5-7].
References:
[1]. Perelman A, Wachtel C, et,al. JC-1: alternative excitation wavelengths facilitate mitochondrial membrane potential cytometry. Cell Death Dis. 2012 Nov 22;3(11):e430. doi: 10.1038/cddis.2012.171. PMID: 23171850; PMCID: PMC3542606.
[2]. Chazotte B. Labeling mitochondria with JC-1. Cold Spring Harb Protoc. 2011 Sep 1;2011(9):pdb.prot065490. doi: 10.1101/pdb.prot065490. PMID: 21880824.
[3]. Armon-Omer A, Neuman H, et,al.Mitochondrial activity is impaired in lymphocytes of MS patients in correlation with disease severity. Mult Scler Relat Disord. 2020 Jun;41:102025. doi: 10.1016/j.msard.2020.102025. Epub 2020 Feb 25. PMID: 32146432.
[4]. Gravance CG, Garner DL, et,al. Assessment of equine sperm mitochondrial function using JC-1. Theriogenology. 2000 Jun;53(9):1691-703. doi: 10.1016/s0093-691x(00)00308-3. PMID: 10968415.
[5].Legrand O, Perrot JY, et,al. JC-1: a very sensitive fluorescent probe to test Pgp activity in adult acute myeloid leukemia. Blood. 2001 Jan 15;97(2):502-8. doi: 10.1182/blood.v97.2.502. PMID: 11154229.
[6]. Kühnel JM, Perrot JY, et,al. Functional assay of multidrug resistant cells using JC-1, a carbocyanine fluorescent probe. Leukemia. 1997 Jul;11(7):1147-55. doi: 10.1038/sj.leu.2400698. PMID: 9205004.
[7].Smiley ST, Reers M, et,al. Intracellular heterogeneity in mitochondrial membrane potentials revealed by a J-aggregate-forming lipophilic cation JC-1. Proc Natl Acad Sci U S A. 1991 May 1;88(9):3671-5. doi: 10.1073/pnas.88.9.3671. PMID: 2023917; PMCID: PMC51514.
Protocol for mitochondrial membrane potential cell assay [1]: |
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Protocol for functional assay JC-1 for P-glycoprotein (Pgp) [2]: |
This protocol only provides a guideline, and should be modified according to your specific needs. |
References: [1]. Perelman A, Wachtel C, et,al. JC-1: alternative excitation wavelengths facilitate mitochondrial membrane potential cytometry. Cell Death Dis. 2012 Nov 22;3(11):e430. doi: 10.1038/cddis.2012.171. PMID: 23171850; PMCID: PMC3542606. [2]. Legrand O, Perrot JY, et,al. JC-1: a very sensitive fluorescent probe to test Pgp activity in adult acute myeloid leukemia. Blood. 2001 Jan 15;97(2):502-8. doi: 10.1182/blood.v97.2.502. PMID: 11154229. |
Cas No. | 3520-43-2 | SDF | |
Synonyms | CBIC2;JC1;JC 1 | ||
Chemical Name | 5,6-dichloro-2-[(E)-3-(5,6-dichloro-1,3-diethylbenzimidazol-3-ium-2-yl)prop-2-enylidene]-1,3-diethylbenzimidazole;iodide | ||
Canonical SMILES | CCN1C2=CC(=C(C=C2[N+](=C1C=CC=C3N(C4=CC(=C(C=C4N3CC)Cl)Cl)CC)CC)Cl)Cl.[I-] | ||
Formula | C25H27Cl4IN4 | M.Wt | 652.23 |
Solubility | ≥ 32.6 mg/mL in DMSO with gentle warming | Storage | Store at -20°C,protect from light |
General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. |
Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
1 mM | 1.5332 mL | 7.666 mL | 15.332 mL |
5 mM | 0.3066 mL | 1.5332 mL | 3.0664 mL |
10 mM | 0.1533 mL | 0.7666 mL | 1.5332 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
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- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5
(Based on Reviews and 30 reference(s) in Google Scholar.)GLPBIO products are for RESEARCH USE ONLY. Please make sure your review or question is research based.
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