Stachyose |
Catalog No.GC18245
One-Click Copy Product Info
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Stachyose is an oligosaccharide and prebiotic.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 470-55-3
Sample solution is provided at 25 µL, 10mM.
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Stachyose is an oligosaccharide and prebiotic[1]. Stachyose also serves as a specific inhibitor of PRDX5 (peroxiredoxin 5), acts directly on the PRDX5 protein, and inhibits its enzymatic activity[2]. Stachyose is commonly used in research on castration-resistant prostate cancer and metabolic diseases such as type 2 diabetes[3][4].
In vitro, Stachyose (0.4-3.2mg/mL in medium; 24h) dose-dependently inhibited Caco-2 colon cancer cells proliferation, induced cells apoptosis, decreased mitochondrial membrane potential, upregulated Bax, downregulated Bcl-2, promoted cytochrome C release, and activated caspases 3/9[5].
In vivo, Stachyose (200-600mg/kg/day; oral gavage for 3 weeks) dose-dependently alleviated DSS-induced colitis symptoms, increased tight junction protein expression (Occludin, Claudin-1, ZO-1), decreased pro-inflammatory cytokines (TNF-α, IL-1β, IL-6), increased anti-inflammatory cytokine IL-10, and restored Treg/Th17 cell balance in a DSS-induced colitis mouse model[6]. Stachyose (1 or 5g/kg/day; oral gavage for 3 days) reduced myocardial infarction area and improved cardiac function in a mouse model of myocardial ischemia-reperfusion injury[7].
References:
[1] Li CN, Wang X, Lei L, et al. Berberine combined with stachyose induces better glycometabolism than berberine alone through modulating gut microbiota and fecal metabolomics in diabetic mice. Phytother Res. 2020;34(5):1166-1174.
[2] Wang R, Pan Y, Zhang L, et al. Prebiotic stachyose inhibits PRDX5 activity and castration-resistant prostate cancer development. Int J Biol Macromol. 2024;278(Pt 3):134844.
[3] Wang R, Mi Y, Ni J, et al. Identification of PRDX5 as A Target for The Treatment of Castration-Resistant Prostate Cancer. Adv Sci (Weinh). 2024;11(9):e2304939.
[4] Wu Y, Cao Y, Feng L, et al. The natural compound stachyose targets SGLT2-mediated metabolic reprogramming to ameliorate diabetic kidney disease. Phytomedicine. 2025;147:157182.
[5] Huang G, Mao J, Ji Z, Ailati A. Stachyose-induced apoptosis of Caco-2 cells via the caspase-dependent mitochondrial pathway. Food Funct. 2015;6(3):765-771.
[6] He N, Chen K, Yu S, et al. Stachyose Exerts Anticolitis Efficacy by Re-balancing Treg/Th17 and Activating the Butyrate-Derived PPARγ Signaling Pathway. J Agric Food Chem. 2024;72(21):12171-12183.
[7] Zhang AY, Su JB, Sun HT, et al. Stachyose ameliorates myocardial ischemia-reperfusion injury by inhibiting cardiomyocyte ferroptosis and macrophage pyroptosis. Int Immunopharmacol. 2024;143(Pt 1):113334.
| Cell experiment [1]: | |
Cell lines | Caco-2 cells |
Preparation Method | Caco-2 cells from passages 30 to 50 were used in this experiment. After reaching subconfluence, Caco-2 cells were recovered from the culture flask through 0.25% trypsin digestion after washing twice with PBS. The medium was changed every 2d. Cell counts based on conductivity measurements and cell viability were assessed using the trypan blue test. To evaluate the cytotoxic effect of Stachyose, cells in the log phase were seeded in 96-well cell culture plates (104 cells per well) and pre-incubated for 24h. The cells were then treated with Serum free medium (SFM) mixed with different Stachyose concentrations (0, 0.4, 0.8, 1.6, 3.2mg/mL) for 24h. The MTT assay was used to measure cell viability according to the commercially available protocol. Flow cytometry analysis for cell apoptosis was performed by using an annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) apoptosis detection kit. |
Reaction Conditions | 0.4-3.2mg/mL; 24h |
Applications | Stachyose dose-dependently inhibited Caco-2 colon cancer cells proliferation and induced cells apoptosis. |
| Animal experiment [2]: | |
Animal models | Male C57BL/6J mice |
Preparation Method | Male C57BL/6J mice, aged 6 weeks were housed in a controlled environment with regulated humidity and temperature, and were provided free access to standard chow and clean water on a 12-h light-dark cycle. After one week acclimatization, the effects of Stachyose on a mouse model of MIRI were evaluated. Stachyose (1 or 5g/kg/day) was administered via gavage for three consecutive days before sham or MIRI surgery. The MIRI model was established under clean and standardized conditions. Echocardiography was performed using the Vevo 3100LT ultrasound cardiography system. After the echocardiography was completed, the mice were euthanized by cervical dislocation, the chest skin and ribs were incised, and the apex of the heart was rinsed with physiological saline. The hearts were then removed and frozen at -20℃ for 20min. Once the hearts were fully frozen, it was dissected using a surgical tool. The hearts were then soaked in 2,3,5-triphenyltetrazolium chloride (TTC dye) at 37℃ for 30min. The necrotic area appeared white, while the viable myocardium was red. After being incubated in 4% paraformaldehyde for 24h, the heart was photographed against a white background. |
Dosage form | 1 or 5g/kg/day; oral gavage for 3 days |
Applications | Stachyose reduced myocardial infarction area and improved cardiac function in a mouse model of myocardial ischemia-reperfusion injury. |
References: | |
| Cas No. | 470-55-3 | SDF | |
| Formula | C24H42O21 | M.Wt | 666.58 |
| Solubility | H2O : 125 mg/mL (Need ultrasonic) | Storage | Store at RT |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.5002 mL | 7.501 mL | 15.002 mL |
| 5 mM | 300 μL | 1.5002 mL | 3.0004 mL |
| 10 mM | 150 μL | 750.1 μL | 1.5002 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >80.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 3 reference(s) in Google Scholar.)