TCEP hydrochloride (Synonyms: Tris(2-carboxyethyl) phosphine hydrochloride) |
Catalog No.GC10529
One-Click Copy Product Info
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TCEP hydrochloride is a non-thiol reducing agent which does not bind Hg2+.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 51805-45-9
Sample solution is provided at 25 µL, 10mM.
TCEP hydrochloride is a non-thiol reducing agent which does not bind Hg2+ [1]. TCEP hydrochloride increased SOD1 monomer formation, thus preventing the NO‐induced increase in dismutase activity and the decrease in ROS[2].
In vitro efficacy test it shown that TCEP hydrochloride promoted NF-kappaB-DNA binding in a dose-related manner in concentrations from 0.25 to 6mM[1].
In vitro experiment it indicated that treatment with 100μM TCEP hydrochloride can improve the quality and developmental capacity of in vitro-fertilized embryos by decreasing oxidative stress in porcine oocytes[3]. In SH-SY5Y cell line, 1mM TCEP hydrochloride maximally protected against BoNT/B inhibition of [(3)H]-NA release, and had no toxic[4]. TCEP hydrochloride (0.01mM) didn't scavenge Fe3+ from Tf and is able to protect thiol groups for the coupling to maleimide. In addition, TCEP hydrochloride didn't interfere with the maleimide coupling itself[5]. In vitro, human hepatocellular (HepG2) cells were treated with 100, 200, and 400μM TCEP hydrochloride for 3 days, the level of oxidative stress, esterase, Ca2+ influx, and ΔΨm dysfunction increased. And there were 65.96% subG1 apoptotic peak in 400μM treated cells[6].
In vivo, treatment with 20mg/kg and 60mg/kg TCEP hydrochloride orally in adult ICR mice for 9 weeks, TCEP improved body weight gain, hypertriglyceridemia, and hepatic steatosis, consistent with upregulation of hepatic lipogenesis-related gene expression. TCEP hydrochloride also altered the levels of several hepatic metabolites[7].
References:
[1] Dieguez-Acuña FJ, et al. Inhibition of NF-kappaB-DNA binding by mercuric ion: utility of the non-thiol reductant, tris(2-carboxyethyl)phosphine hydrochloride (TCEP hydrochloride), on detection of impaired NF-kappaB-DNA binding by thiol-directed agents. Toxicol In Vitro. 2000 Feb;14(1):7-16.
[2] Peng H, et al. Nitric oxide inhibits endothelial cell apoptosis by inhibiting cysteine-dependent SOD1 monomerization. FEBS Open Bio. 2022 Feb;12(2):538-548.
[3] Zeng Y, et al. Effects of tris (2-carboxyethyl) phosphine hydrochloride treatment on porcine oocyte in vitro maturation and subsequent in vitro fertilized embryo developmental capacity. Theriogenology. 2021 Mar 1;162:32-41.
[4] Shi X, et al. TCEP hydrochloride treatment reduces proteolytic activity of BoNT/B in human neuronal SHSY-5Y cells. J Cell Biochem. 2009 Aug 1;107(5):1021-30.
[5] Visser CC, et al. Coupling of metal containing homing devices to liposomes via a maleimide linker: use of TCEP to stabilize thiol-groups without scavenging metals. J Drug Target. 2004;12(9-10):569-73.
[6] M Al-Salem A, et al. Tris(2-chloroethyl) Phosphate (TCEP) Elicits Hepatotoxicity by Activating Human Cancer Pathway Genes in HepG2 Cells. Toxics. 2020 Nov 20;8(4):109.
[7] Yang D, et al. Tris (2-chloroethyl) phosphate (TCEP) induces obesity and hepatic steatosis via FXR-mediated lipid accumulation in mice: Long-term exposure as a potential risk for metabolic diseases. Chem Biol Interact. 2022 Aug 25;363:110027.
| Cell experiment [1]: | |
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Cell lines |
HUVEC cells |
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Preparation Method |
The NO donor SNP (100µM), the NOS inhibitor L‐NAME (500µM, and the cysteine thiol‐reducing agent TCEP hydrochloride hydrochloride (2mM) were used to HUVEC cells. |
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Reaction Conditions |
2mM; 2h |
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Applications |
TCEP hydrochloride successfully abolished NO‐induced inhibition of SOD1 monomerization in HUVECs, indicating that NO inhibited monomeric SOD1 by acting on cysteine thiol |
| Animal experiment [2]: | |
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Animal models |
Adult Kunming mice |
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Preparation Method |
Total 30 adult Kunming mice were randomly divided into normal control group (0mg/kg·d), low-dose TCEP hydrochloride group (10mg/kg·d), and high-dose TCEP hydrochloride group (100mg/kg·d), and administered continuously by gavage for 30 days. |
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Dosage form |
0, 10, 100mg/kg·d; 30 days; p.o. |
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Applications |
Compared with the control group, the water intake of high-dose TCEP hydrochloride group was declined significantly, and the organ index of liver and spleen were increased significantly. In addition, the escape latency of TCEP hydrochloride exposed mice were longer than that in the control group in water maze test, while the total swimming course of high-dose TCEP hydrochloride group was elevated and the swimming time in target quadrant was obviously shortened compared with the control group. |
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References: |
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| Cas No. | 51805-45-9 | SDF | |
| Synonyms | Tris(2-carboxyethyl) phosphine hydrochloride | ||
| Chemical Name | 3,3',3''-phosphinetriyltripropanoic acid hydrochloride | ||
| Canonical SMILES | O=C(O)CCP(CCC(O)=O)CCC(O)=O.Cl | ||
| Formula | C9H16ClO6P | M.Wt | 286.65 |
| Solubility | ≥ 28.7mg/mL in Water ; ≥ 50mg/mL in DMSO | Storage | Store at 2-8°C , protect from light, stored under nitrogen |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.4886 mL | 17.4429 mL | 34.8857 mL |
| 5 mM | 697.7 μL | 3.4886 mL | 6.9771 mL |
| 10 mM | 348.9 μL | 1.7443 mL | 3.4886 mL |
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Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
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Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 6 reference(s) in Google Scholar.)