Terbutaline |
Catalog No.GC45993
One-Click Copy Product Info
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Terbutaline is a novel selective β2-adrenergic receptor agonist.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 23031-25-6
Sample solution is provided at 25 µL, 10mM.
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Terbutaline is a novel selective β2-adrenergic receptor agonist. Terbutaline can dilate bronchial smooth muscle by activating adenylate cyclase to increase intracellular cyclic adenosine monophosphate (cAMP) levels. Terbutaline reduces smooth muscle contraction and inflammation by activating protein kinase A (PKA) and inhibiting myosin light chain kinase (MLCK). Terbutaline is used for research on asthma, chronic obstructive pulmonary disease (COPD), and preterm labor[1-4].
In vitro, Terbutaline (10μM) repeatedly activated CD3+ T cells (for 48h each time; 4 times in total). Terbutaline had no significant effect on the expression of T cell exhaustion markers (CD4+TIM-3+CD38+ and CD8+TIM-3+CD38+) or cytotoxicity[5]. Terbutaline (0.1nM–10μM) was pretreated on J774 mouse macrophages for 30min, followed by stimulation with LPS (10ng/mL) for 1h. Terbutaline significantly inhibited the phosphorylation of p38 MAPK and reduced TNF production[6].
In vivo, Terbutaline (1–10μg; 5μL) was administered to male Hsd: CD-1 (ICR) mice via intrathecal injection (i.t.; single dose). Terbutaline significantly increased blood glucose levels and plasma corticosterone levels[7]. Terbutaline (500μg/kg/day; twice daily) was intraperitoneally injected into collagen-induced arthritis (CIA) DBA1/J mice for 16 days. Terbutaline significantly alleviated arthritis symptoms in CIA mice, reduced the histopathological injuries of the ankle joints, decreased the arthritis score of the four limbs, and reduced the thickness of the ankle joints and rear paws[8].
References:
[1] Mohamed Ismail NA, Ibrahim M, Mohd Naim N, et al. Nifedipine versus terbutaline for tocolysis in external cephalic version. Int J Gynaecol Obstet. 2008 Sep;102(3):263-6.
[2] Loureiro JA, Andrade S, Ramalho MJ, et al. The interaction of a β2 adrenoceptor agonist drug with biomimetic cell membrane models: The case of terbutaline sulphate. Life Sci. 2021 Nov 15;285:119992.
[3] Karni R, Mizrachi S, Reiss-Sklan E, et al. The pp60c-Src inhibitor PP1 is non-competitive against ATP. FEBS Lett. 2003 Feb 27;537(1-3):47-52.
[4] Choucair-Jaafar N, Salvat E, Freund-Mercier MJ, et al. The antiallodynic action of nortriptyline and terbutaline is mediated by β(2) adrenoceptors and δ opioid receptors in the ob/ob model of diabetic polyneuropathy. Brain Res. 2014 Feb 10;1546:18-26.
[5] Hajiaghayi M, Gholizadeh F, Rahbari N, et al. Nebivolol prevents exhausted T cells and enhances cytotoxicity against MCF-7 breast cancer cells in a β2-adrenergic receptor-dependent manner. Clin Exp Immunol. 2026 Jan 6;220(1):uxag018.
[6] Keränen T, Hömmö T, Hämäläinen M, et al. Anti-Inflammatory Effects of β2-Receptor Agonists Salbutamol and Terbutaline Are Mediated by MKP-1. PLoS One. 2016 Feb 5;11(2):e0148144.
[7] Sim YB, Park SH, Kim SS, et al. Spinal β-adrenergic receptors' activation increases the blood glucose level in mice. Anim Cells Syst (Seoul). 2017 Jul 10;21(4):278-285.
[8] Lu JH, Rui XX, Wang TT, et al. Activation of β2-adrenergic Receptor Alleviates Collagen-induced Arthritis by Ameliorating Th17/Treg Imbalance. Iran J Immunol. 2023 Mar 14;20(1):16-25.
| Cell experiment [1]: | |
Cell lines | J774 mouse macrophages |
Preparation Method | J774 macrophages were incubated with Terbutaline (0.1nM-10μM) for the indicated times. |
Reaction Conditions | 0.1nM-10μM; pre-incubation for 30 minutes before LPS (10ng/mL) stimulation |
Applications | Terbutaline significantly increased intracellular cAMP levels in J774 macrophages. Terbutaline also enhanced MKP-1 mRNA and protein expression in a dose-dependent manner. Terbutaline markedly inhibited LPS-induced p38 MAPK phosphorylation and suppressed both mRNA expression and protein production of TNF. |
| Animal experiment [2]: | |
Animal models | Male DBA1/J mice (8-10 weeks old, weighing 20-25g) |
Preparation Method | Mice were intradermally injected with type II collagen (CII) emulsion at the tail base on day 0, followed by an intraperitoneal injection of CII emulsions on day 21, and a lipopolysaccharide boost on day 28 to induce the collagen-induced arthritis (CIA) model. Terbutaline (500μg/kg/day) administration for 16 days. |
Dosage form | 500μg/kg/day; i.p.; twice a day |
Applications | Terbutaline administration significantly alleviated arthritis symptoms in the CIA mice, including markedly reducing the histopathological injuries of the ankle joints, notably decreasing the arthritis score of the four limbs, and significantly reducing the thickness of the ankle joints and rear paws. |
References: | |
| Cas No. | 23031-25-6 | SDF | |
| Chemical Name | 5-[2-[(1,1-dimethylethyl)amino]-1-hydroxyethyl]-1,3-benzenediol | ||
| Canonical SMILES | OC1=CC(O)=CC(C(O)CNC(C)(C)C)=C1 | ||
| Formula | C12H19NO3 | M.Wt | 225.3 |
| Solubility | DMSO: slightly soluble,Methanol: slightly soluble,Water: 100 mM | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 4.4385 mL | 22.1926 mL | 44.3853 mL |
| 5 mM | 887.7 μL | 4.4385 mL | 8.8771 mL |
| 10 mM | 443.9 μL | 2.2193 mL | 4.4385 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
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Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 28 reference(s) in Google Scholar.)