Triclosan |
Catalog No.GC13311
One-Click Copy Product Info
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Triclosan is a broad-spectrum antibacterial agent.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 3380-34-5
Sample solution is provided at 25 µL, 10mM.
Triclosan is a broad-spectrum antibacterial agent[1]. Triclosan inhibits bacterial fatty acid synthesis at the enoyl-acyl carrier protein reductase (FabI) step[2]. Triclosan is commonly used for antibacterial research and is widely found in daily necessities such as soaps, detergents, toys, and surgical cleaning treatments[3][4].
In vitro, Triclosan (1-100µM; 1-24h) decreased cell viability and induced cell apoptosis in a dose- and time-dependent manner in rat neural stem cells (NSCs)[5].
In vivo, Triclosan (50-200mg/kg/day; p.o.; 7 days) significantly reduced serum testosterone levels, downregulated the expression of Leydig cell genes involved in testosterone biosynthesis in adult male rats[6]. Triclosan (80ppm in diet; 4 weeks) increased the severity of dextran sodium sulfate (DSS)-induced colitis, and promoted colonic inflammation and immune cell infiltration in mice[7].
References:
[1] Vosatka R, Kratky M, Vinsova J. Triclosan and its derivatives as antimycobacterial active agents. Eur J Pharm Sci. 2018;114:318-331.
[2] Heath RJ, Rubin JR, Holland DR, Zhang E, Snow ME, Rock CO. Mechanism of triclosan inhibition of bacterial fatty acid synthesis. J Biol Chem. 1999;274(16):11110-11114.
[3] Shrestha P, Zhang Y, Chen WJ, Wong TY. Triclosan: antimicrobial mechanisms, antibiotics interactions, clinical applications, and human health. J Environ Sci Health C Toxicol Carcinog. 2020;38(3):245-268.
[4] Weatherly LM, Gosse JA. Triclosan exposure, transformation, and human health effects. J Toxicol Environ Health B Crit Rev. 2017;20(8):447-469.
[5] Park BK, Gonzales EL, Yang SM, Bang M, Choi CS, Shin CY. Effects of Triclosan on Neural Stem Cell Viability and Survival. Biomol Ther (Seoul). 2016;24(1):99-107.
[6] Sang J, Ji Z, Li H, et al. Triclosan inhibits testosterone biosynthesis in adult rats via inducing m6A methylation-mediated autophagy. Environ Int. 2024;190:108827.
[7] Zhang J, Walker ME, Sanidad KZ, et al. Microbial enzymes induce colitis by reactivating triclosan in the mouse gastrointestinal tract. Nat Commun. 2022;13(1):136.
| Cell experiment [1]: | |
Cell lines | Neural stem cells |
Preparation Method | Neural stem cells were cultured from embryonic day 14 (E14) brain of Sprague-Dawley rat. In brief, cortices were dissociated into single cells by mechanical trituration, and cells were incubated in DMEM/F12 supplemented with B27-serum free supplement and growth factor (10ng/ml FGF and 20ng/ml EGF) in a 5% CO2 incubator. EGF and FGF were added every day and the cells grew into floating neurospheres, which were dissociated into single cells using trypsin-EDTA (0.1%) and plated into poly-L-ornithine pre-coated plates using B27- medium without growth factors for differentiation. Neural stem wells were treated in vitro with Triclosan at 1, 10, 20, 30, 50 and 100μM concentrations and applied into the culture media at different lengths of exposure (3, 6, 8, 12 and 24h) to determine the dose and time-dependent effects. All drug treatments and experiments were conducted during the 4th day of in vitro culture (DIV4) of NSCs. To evaluate neuronal cell death in culture Triclosan treatment, PI staining was performed. MTT assay was used to examine whether Triclosan treatment can influence NSC proliferation. |
Reaction Conditions | 1-100µM; 1-24h |
Applications | Triclosan decreased cell viability and induced cell apoptosis in a dose- and time-dependent manner in rat neural stem cells (NSCs). |
| Animal experiment [2]: | |
Animal models | Adult male Sprague-Dawley rats |
Preparation Method | 24 adult male Sprague-Dawley rats (56 days old) rats were housed in cages free from Triclosan following a one-week acclimatization period, under the conditions of temperature maintained at 23±2℃, humidity levels ranging from 45% to 55%, and a 12h-12h light-dark cycle. The rats were randomly divided into four groups consisting of 6 rats in each group: control group (corn oil), 50mg/kg/day, 100mg/kg/day, and 200mg/kg/day Triclosan groups. Each dose of Triclosan was mixed with 1ml of corn oil and administered to the rats at a rate of 1ml per kilogram of body weight. The administration regimen for the rats extended from postnatal day (PND) 56 to PND 63, a timeframe that aligns with the transition to adulthood. This period was selected to assess the potential effects of Triclosan exposure during a critical developmental window on subsequent hormonal regulation. The body weight of each rat was recorded throughout the study. At the end of the Triclosan exposure period, the rats were euthanized using CO2 followed by cervical dislocation. Trunk blood was collected, centrifuged at 1500×g for 10min to obtain serum. The testes and epididymis were removed and weighed. One testis was stored at -80℃ for mRNA and protein analysis, while the other testis was fixed in Bouin’s fixative for immunohistochemistry. Sperm counting was performed using the cauda epididymis. |
Dosage form | 50-200mg/kg/day; p.o.; 7 days |
Applications | Triclosan significantly reduced serum testosterone levels, downregulated the expression of Leydig cell genes involved in testosterone biosynthesis in adult male rats. |
References: | |
| Cas No. | 3380-34-5 | SDF | |
| Chemical Name | 5-chloro-2-(2,4-dichlorophenoxy)-phenol | ||
| Canonical SMILES | ClC1=C(OC2=CC=C(Cl)C=C2O)C=CC(Cl)=C1 | ||
| Formula | C12H7Cl3O2 | M.Wt | 289.5 |
| Solubility | ≥ 10.95mg/mL in DMSO | Storage | 4°C, protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.4542 mL | 17.2712 mL | 34.5423 mL |
| 5 mM | 690.8 μL | 3.4542 mL | 6.9085 mL |
| 10 mM | 345.4 μL | 1.7271 mL | 3.4542 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
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- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 22 reference(s) in Google Scholar.)