Alda 1 |
Catalog No.GC16597
One-Click Copy Product Info
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Alda 1 is an aldehyde dehydrogenase 2 (ALDH2) activator.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 349438-38-6
Sample solution is provided at 25 µL, 10mM.
Alda 1 is an aldehyde dehydrogenase 2 (ALDH2) activator [1]. Alda 1 specifically activating the ALDH2 enzyme in mitochondria, enhances the enzyme's ability to metabolize toxic aldehydes such as acetaldehyde, thereby reducing cellular damage caused by these substances [2-3]. Alda 1 is primarily used to study diseases associated with abnormal acetaldehyde metabolism [4].
In human pulmonary alveolar epithelial cells, Alda 1 (20μM; 1h) pretreatment can alleviate lung ischemia-reperfusion injury [5]. In SH-SY5Y cells, pretreatment with Alda 1 (20 μM; 24 h) restored ALDH2 activity and content, increased mitochondrial membrane potential, and reduced reactive oxygen species in cells [6].
In C57BL/6J mice, after Alda 1 (5mg/kg; ip; single injection) administration elevated hepatic ALDH activity, which was blocked by the specific ALDH2 inhibitor, daidzin [7]. In liver ischemia-reperfusion injury (IRI) mice model, Alda 1 (30mg/kg; ip; single injection) mediated ALDH2 activation may improve hepatic IRI by scavenging reactive aldehydes and enhancing autophagy [8]. In hyperoxia mice model, Alda 1 (8mg/kg; sc; 48h) pretreatment attenuates hyperoxia-induced immune cell infiltration [9].
References:
[1]. Stachowicz A, Olszanecki R, Suski M, et al. Proteomic analysis of mitochondria-enriched fraction isolated from the frontal cortex and hippocampus of apolipoprotein E knockout mice treated with Alda-1, an activator of mitochondrial aldehyde dehydrogenase (ALDH2)[J]. International Journal of Molecular Sciences, 2017, 18(2): 435.
[2]. Stachowicz A, Głombik K, Olszanecki R, et al. The impact of mitochondrial aldehyde dehydrogenase (ALDH2) activation by Alda-1 on the behavioral and biochemical disturbances in animal model of depression[J]. Brain, Behavior, and Immunity, 2016, 51: 144-153.
[3]. Ikeda T, Takahashi T, Tsujita M, et al. Effects of Alda-1, an aldehyde dehydrogenase-2 agonist, on hypoglycemic neuronal death[J]. PLoS One, 2015, 10(6): e0128844.
[4]. Stachowicz A, Olszanecki R, Suski M, et al. Mitochondrial aldehyde dehydrogenase activation by Alda‐1 inhibits atherosclerosis and attenuates hepatic steatosis in apolipoprotein E‐knockout mice[J]. Journal of the American Heart Association, 2014, 3(6): e001329.
[5]. Ding J, Zhang Q, Luo Q, et al. Alda-1 attenuates lung ischemia-reperfusion injury by reducing 4-hydroxy-2-nonenal in alveolar epithelial cells[J]. Critical care medicine, 2016, 44(7): e544-e552.
[6]. Deza-Ponzio R, Albrecht P A, Fernandez-Hubeid L E, et al. ALDH2 inhibition by lead and ethanol elicits redox imbalance and mitochondrial dysfunction in SH-SY5Y human neuroblastoma cell line: Reversion by Alda-1[J]. Neurotoxicology, 2023, 97: 12-24.
[7]. Zhong W, Zhang W, Li Q, et al. Pharmacological activation of aldehyde dehydrogenase 2 by Alda-1 reverses alcohol-induced hepatic steatosis and cell death in mice[J]. Journal of hepatology, 2015, 62(6): 1375-1381.
[8]. Li M, Xu M, Li J, et al. Alda‐1 Ameliorates Liver Ischemia‐Reperfusion Injury by Activating Aldehyde Dehydrogenase 2 and Enhancing Autophagy in Mice[J]. Journal of Immunology Research, 2018, 2018(1): 9807139.
[9]. Sidramagowda Patil S, Hernández-Cuervo H, Fukumoto J, et al. Alda-1 attenuates hyperoxia-induced acute lung injury in mice[J]. Frontiers in pharmacology, 2021, 11: 597942.
| Cell experiment [1]: | |
Cell lines | Human pulmonary alveolar epithelial cells |
Preparation Method | Primary human pulmonary alveolar epithelial cells were cultured at 37℃ and 5% CO2, in alveolar epithelial cell medium and endothelial cell-growth medium MV2. Hypoxia was induced by exposing the cells for 24 hours to serum-deprived media in a sealed anaerobic jar fitted with an AnaeroPack to scavenge any free oxygen. The cells were then washed with PBS and were reoxygenated for 2 hours under normal conditions in complete medium. Alda 1, 20μM, was added to the medium for 1 hour before hypoxic exposure of the hypoxia/reoxygenation (H/R) + Alda 1 group. |
Reaction Conditions | 20μM; 1h |
Applications | Alda 1 pretreatment can alleviate lung ischemia-reperfusion injury. |
| Animal experiment [2]: | |
Animal models | C57BL/6J mice |
Preparation Method | Mice were given 5mg/kg body weight (BW) Alda 1 (dissolved in dimethyl sulfoxide, DMSO) at 9:00 am via three routes, intraperitoneal injection (i.p.). Livers were collected 3 hours after treatments, and hepatic ALDH activity was immediately measured. To assess the specificity of Alda 1 on activating ALDH2, mice were given an ALDH2 specific inhibitor, daidzin, at 50mg/kg BW (dissolved in DMSO) via i.p. along with 5mg/kg BW Alda 1 administration at 9:00 am (n = 4). Three hours later, liver and ileum were collected, mitochondrial and cytosolic fractions from the liver were isolated, and ALDH activity was immediately measured. |
Dosage form | 5mg/kg; ip; single injection |
Applications | One dose of Alda 1 administration elevated hepatic ALDH activity, which was blocked by the specific ALDH2 inhibitor, daidzin. |
References: | |
| Cas No. | 349438-38-6 | SDF | |
| Chemical Name | N-(benzo[d][1,3]dioxol-5-ylmethyl)-2,6-dichlorobenzamide | ||
| Canonical SMILES | ClC1=CC=CC(Cl)=C1C(NCC2=CC=C3OCOC3=C2)=O | ||
| Formula | C15H11Cl2NO3 | M.Wt | 324.16 |
| Solubility | ≥ 15.15mg/mL in DMSO | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.0849 mL | 15.4245 mL | 30.849 mL |
| 5 mM | 617 μL | 3.0849 mL | 6.1698 mL |
| 10 mM | 308.5 μL | 1.5424 mL | 3.0849 mL |
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
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- Purity: >99.50% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
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Average Rating: 5 (Based on Reviews and 20 reference(s) in Google Scholar.)