Fisetin (Synonyms: CI-75620, NSC 407010, NSC 656275) |
| Catalog No.GN10030 |
Fisetin is a natural flavonol found in many fruits and vegetables with multiple biological activities.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 528-48-3
Sample solution is provided at 25 µL, 10mM.
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Fisetin is a natural flavonol found in many fruits and vegetables with multiple biological activities[1]. Fisetin is a strong antioxidant and can be used as an effective anti-aging agent[2]. Fisetin can be used as a chemotherapeutic agent for various cancers and as a neuroprotectant[3].
In vitro, treatment of human retinoblastoma Y79 cell line with Fisetin (25, 50, 100µM) for 24-72h inhibited cell viability and proliferation, cell invasion and migration, and intracellular vascular endothelial growth factor receptor (VEGFR) expression in a time- and dose-dependent manner[4]. Treatment of glioma cells (T98G and BEAS-2B cells) with Fisetin (1-500μM) for 24h and 48h inhibited cell proliferation and induced cell apoptosis and necrosis in a time- and dose-dependent manner[5]. Fisetin (0-40μM) treated A549 cells for 24-72h, inhibited cell proliferation in a time- and dose-dependent manner, led to cell cycle arrest, and inhibited cell adhesion, invasion, and migration[6].
In vivo, Fisetin (10, 20mg/kg) was orally treated for 10 days in mice with acute otitis media, significantly downregulated the mRNA levels of IL-1β, TNF-α, IL-6, and VEGF in mouse serum and middle ear tissue in a dose-dependent manner, upregulated the protein levels of SOD1, SOD2, HO-1, and Nrf2, and improved the inflammatory damage of the middle ear in mice[7]. Fisetin (223mg/kg) was treated with 4T1 breast tumor model mice by intraperitoneal injection for 3 weeks, inhibited the growth of 4T1 cell-derived in situ breast tumors, and promoted tumor cell apoptosis[8].
References:
[1] Antika L D, Dewi R M. Pharmacological aspects of fisetin[J]. Asian Pacific Journal of Tropical Biomedicine, 2021, 11(1): 1-9.
[2] Yousefzadeh M J, Zhu Y I, McGowan S J, et al. Fisetin is a senotherapeutic that extends health and lifespan[J]. EBioMedicine, 2018, 36: 18-28.
[3] Khan N, Syed D N, Ahmad N, et al. Fisetin: a dietary antioxidant for health promotion[J]. Antioxidants & redox signaling, 2013, 19(2): 151-162.
[4] Wang L, Chen N, Cheng H. Fisetin inhibits vascular endothelial growth factor induced angiogenesis in retinoblastoma cells[J]. Oncology Letters, 2020, 20(2): 1239-1244.
[5] Pak F, Oztopcu-Vatan P. Fisetin effects on cell proliferation and apoptosis in glioma cells[J]. Zeitschrift für Naturforschung C, 2019, 74(11-12): 295-302.
[6] Wang J, Huang S. Fisetin inhibits the growth and migration in the A549 human lung cancer cell line via the ERK1/2 pathway[J]. Experimental and Therapeutic Medicine, 2018, 15(3): 2667-2673.
[7] Li P, Chen D, Huang Y. Fisetin administration improves LPS-induced acute otitis media in mouse in vivo[J]. International journal of molecular medicine, 2018, 42(1): 237-247.
[8] Sun X, Ma X, Li Q, et al. Anti cancer effects of fisetin on mammary carcinoma cells via regulation of the PI3K/Akt/mTOR pathway: In vitro and in vivo studies[J]. International Journal of Molecular Medicine, 2018, 42(2): 811-820.
| Cell experiment [1]: | |
|
Cell lines |
Y79 cells |
|
Preparation Method |
The Y79 cells in the logarithmic growth phase were harvested, digested with 0.25% trypsin, added to RPMI-1640 medium containing 10% serum to prepare a cell suspension of 1×108/ml, plated in 96-well cell culture plates with 90µL per well, and incubated for 24h until the cells grew into a monolayer. Subsequently, 10µl of Fisetin were added at final concentrations of 25, 50 and 100µM with 100µg/ml VEGF. The negative control comprised an equal volume of DMSO with 100µg/ml VEGF. Fisetin was added to the cells for 24, 48 and 72h. The cell viability was determined by CCK-8 method, and the proliferation inhibition rate was calculated. |
|
Reaction Conditions |
25, 50, 100µM; 24, 48, 72h |
|
Applications |
Fisetin inhibited Y79 cell viability and proliferation in a time- and dose-dependent manner. |
| Animal experiment [2]: | |
|
Animal models |
C57BL/6 mice |
|
Preparation Method |
After LPS exposure for 24h, 36 mice with acute otitis media were randomly divided into two groups. Eighteen mice after LPS treatment were given low dose of 10mg/kg of Fisetin dissolved in 5% DMSO in PBS by intragastric administration every day for 10 days, and the other 18 mice a were given high dose of 20mg/kg of Fisetin dissolved in 5% DMSO in PBS through intragastric administration every day for 10 days. Finally, the mice were sacrificed and the eyeball blood and Middle ear lavage fluids (MELF) was collected for following research. |
|
Dosage form |
10、20mg/kg/day for 10 days; p.o. |
|
Applications |
Fisetin administration significantly downregulated the mRNA levels of IL-1β, TNF-α, IL-6, and VEGF in the serum and middle ear tissues of mice in a dose-dependent manner, and upregulated the protein levels of SOD1, SOD2, HO-1, and Nrf2. |
|
References: [1]Wang L, Chen N, Cheng H. Fisetin inhibits vascular endothelial growth factor induced angiogenesis in retinoblastoma cells[J]. Oncology Letters, 2020, 20(2): 1239-1244. [2]Li P, Chen D, Huang Y. Fisetin administration improves LPS-induced acute otitis media in mouse in vivo[J]. International journal of molecular medicine, 2018, 42(1): 237-247. |
|
| Cas No. | 528-48-3 | SDF | |
| Synonyms | CI-75620, NSC 407010, NSC 656275 | ||
| Chemical Name | 2-(3,4-dihydroxyphenyl)-3,7-dihydroxychromen-4-one | ||
| Canonical SMILES | C1=CC(=C(C=C1C2=C(C(=O)C3=C(O2)C=C(C=C3)O)O)O)O | ||
| Formula | C15H10O6 | M.Wt | 286.05 |
| Solubility | ≥ 10.3mg/mL in DMSO | Storage | Store at -20°C,protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 3.4959 mL | 17.4795 mL | 34.9589 mL |
| 5 mM | 699.2 μL | 3.4959 mL | 6.9918 mL |
| 10 mM | 349.6 μL | 1.7479 mL | 3.4959 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)
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