FB23-2 |
Catalog No.GC36032
One-Click Copy Product Info
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FB23-2 is a selective inhibitor of mRNA N6-methyladenine (M6A) demethylase FTO (IC50 = 2.6μM). It is can be used in research related to acute myeloid leukemia (AML).
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 2243736-45-8
Sample solution is provided at 25 µL, 10mM.
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FB23-2 is a selective inhibitor of mRNA N6-methyladenine (M6A) demethylase FTO (IC50 = 2.6μM)[1]. It is can be used in research related to acute myeloid leukemia (AML)[1].
FB23-2 (72 hours) inhibited the proliferation of NB4 and MONOMAC6 cells, with an IC50 of 0.8μM and 1.5μM. FB23-2 (10μM, 96 hours) can significantly enhance the sensitivity of nasopharyngeal carcinoma (NPC) cells to irradiation[2].
FB23-2 (2mg/kg/d, 10 days, i.p.) significantly inhibited the progression of acute myeloid leukemia (AML) and extended survival in AML mice[1]. FB23-2 (8mg/kg/d, 14 days, i.p.) significantly inhibited tumor growth and prolonged survival time in clear cell renal cell carcinoma (ccRCC) mouse model[3]. FB23–2 (2mg/kg/day, 3 days, intrabitoneal injection) aggravated lung injury, the inflammatory response, and ferroptosis in acute lung injury (ALI) mouse model[4].
References:
[1] Huang Y, Su R, Sheng Y, et al. Small-molecule targeting of oncogenic FTO demethylase in acute myeloid leukemia. Cancer cell. 2019 Apr 15;35(4):677-91.
[2] Huang WM, Li ZX, Wu YH, et al. m6A demethylase FTO renders radioresistance of nasopharyngeal carcinoma via promoting OTUB1-mediated anti-ferroptosis. Translational oncology. 2023 Jan 1;27:101576.
[3] Xu Y, Zhou J, Li L, et al. FTO-mediated autophagy promotes progression of clear cell renal cell carcinoma via regulating SIK2 mRNA stability. International Journal of Biological Sciences. 2022;18(15):5943.
[4] Zhao Y, Ding W, Cai Y, et al. The m6A eraser FTO suppresses ferroptosis via mediating ACSL4 in LPS-induced macrophage inflammation. Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease. 2024 Oct 1;1870(7):167354.
| Cell experiment [1]: | |
Cell lines | Nasopharyngeal carcinoma (NPC) cells |
Preparation Method | The cells (2 × 105) were seeded into dishes and cultured for 24h and treated with FB23-2 (10μmol) or transfected with Flag-FTO. After 24h, the cells were irradiated with X-ray beam at 4 Gy and were cultured again for 72h. Finally cellular lipid measurement was confirmed by flow cytometry. |
Reaction Conditions | 10μM, 96 hours |
Applications | FB23-2 significantly sensitized NPC cells to irradiation. |
| Animal experiment [2]: | |
Animal models | Acute myeloid leukemia (AML) mouse model |
Preparation Method | For the AML mouse model, 0.2 × 106 MONOMAC6 cells were directly transplanted into NOD/LtSz-scid IL2RG-SGM3 (NSGS) mice via tail vein. After 10 days, FB23-2 (2mg/kg/day) and DMSO vehicle were intraperitoneally injected into the mice for a continuous 10 days. |
Dosage form | 2mg/kg/d, 10 days, i.p. |
Applications | FB23-2 substantially delayed the onset of full-blown leukemic symptoms and significantly prolonged survival by almost doubling the median survival. |
| Cas No. | 2243736-45-8 | SDF | |
| Canonical SMILES | O=C(NO)C1=CC=CC=C1NC2=C(Cl)C=C(C3=C(C)ON=C3C)C=C2Cl | ||
| Formula | C18H15Cl2N3O3 | M.Wt | 392.24 |
| Solubility | DMSO: 62.5 mg/mL (159.34 mM) | Storage | 4°C, protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.5495 mL | 12.7473 mL | 25.4946 mL |
| 5 mM | 509.9 μL | 2.5495 mL | 5.0989 mL |
| 10 mM | 254.9 μL | 1.2747 mL | 2.5495 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >99.50% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 20 reference(s) in Google Scholar.)