3,3-Dimethyl-1-butanol (Synonyms: DMB; Neohexanol) |
Catalog No.GD09670
One-Click Copy Product Info
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3,3-Dimethyl-1-butanol is an oral inhibitor that inhibits the production of trimethylamine (TMA) by microorganisms and suppresses different microbial TMA lyases.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 624-95-3
Sample solution is provided at 25 µL, 10mM.
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3,3-Dimethyl-1-butanol is an oral inhibitor that inhibits the production of trimethylamine (TMA) by microorganisms and suppresses different microbial TMA lyases[1]. 3,3-Dimethyl-1-butanol can reduce superoxide production in aorta and inhibit the occurrence of atherosclerosis and endothelial dysfunction[2]. 3,3-Dimethyl-1-butanol has been widely used to reduce TMA and gut microbiota-derived metabolite trimethylamine N-oxide (TMAO) formation in animal models[3].
In vitro, 3,3-Dimethyl-1-butanol treatment at 1μM for 24h inhibited secretion of IL-1β and IL-6 from 10µg/µl lipopolysaccharide (LPS)-stimulated mouse bone marrow-derived macrophages[4].
In vivo, 3,3-Dimethyl-1-butanol treatment (1.0% dissolved in drinking water) via oral administration for 8 weeks significantly prevented the impairment of cardiac contraction and relaxation function in mice caused by a high-fat diet[5]. Drinking 1.0% 3,3-Dimethyl-1-butanol water for six consecutive weeks can significantly reduce the plasma TMAO levels in mice undergoing aortic banding surgery, and inhibit adverse cardiac structural remodeling[6]. The continuous consumption of water containing 1% 3,3-Dimethyl-1-butanol for 21 consecutive days led to a significant reduction in the inflammatory response and oxidative stress in the limbs of mice with unilateral hindlimb ischemia, and improved perfusion recovery[7].
References:
[1] Wang Z, Roberts A B, Buffa J A, et al. Non-lethal inhibition of gut microbial trimethylamine production for the treatment of atherosclerosis[J]. Cell, 2015, 163(7): 1585-1595.
[2] Casso A G, VanDongen N S, Ziemba B P, et al. Initiation of the Gut Microbiome Targeted Compound 3, 3‐Dimethyl‐1‐butanol at Mid‐life Prevents Age‐related Vascular Dysfunction[J]. The FASEB Journal, 2020, 34(S1): 1-1.
[3] Iglesias-Carres L, Essenmacher L A, Racine K C, et al. Development of a high-throughput method to study the inhibitory effect of phytochemicals on trimethylamine formation[J]. Nutrients, 2021, 13(5): 1466.
[4] Fechtner S, Allen B E, Chriswell M E, et al. 3, 3-dimethyl-1-butanol and its metabolite 3, 3-dimethylbutyrate ameliorate collagen-induced arthritis independent of choline trimethylamine lyase activity[J]. Inflammation, 2025, 48(3): 1350-1365.
[5] Chen K, Zheng X, Feng M, et al. Gut microbiota-dependent metabolite trimethylamine N-oxide contributes to cardiac dysfunction in western diet-induced obese mice[J]. Frontiers in physiology, 2017, 8: 139.
[6] Wang G, Kong B, Shuai W, et al. 3, 3-Dimethyl-1-butanol attenuates cardiac remodeling in pressure-overload-induced heart failure mice[J]. The Journal of nutritional biochemistry, 2020, 78: 108341.
[7] Chen L, Jin Y, Wang N, et al. Trimethylamine N-oxide impairs perfusion recovery after hindlimb ischemia[J]. Biochemical and Biophysical Research Communications, 2020, 530(1): 95-99.
| Cell experiment [1]: | |
Cell lines | Mouse bone marrow-derived macrophages |
Preparation Method | Bone marrow was flushed and processed to a single cell suspension from the femur and tibia of 6–10-week-old DBA/1j mice using 1ml cold PBS. The single cell suspension was cultured in 9ml of complete RPMI 1640 medium supplemented with 10% fetal bovine serum, 2% HEPES, 0.6% penicillin/streptomycin, 0.1% 2-mercaptoethanol, and 20ng/ml recombinant mouse GM-CSF. After 72h, the culture media were removed and replaced with fresh differentiation media to remove non-adherent cells. After 6 days of culture, the adherent macrophages were washed with cold PBS and resuspended in RPMI 1640 without recombinant mouse GM-CSF. Cells were stimulated with 10µg/µl LPS and treated with different concentrations of 3,3-Dimethyl-1-butanol (0.00001, 0.001, 1, 100, 10000, and 25000nM) for 24h. The secretion levels of proinflammatory cytokines were analyzed. |
Reaction Conditions | 0.00001, 0.001, 1, 10, 1000, 10000, and 25000nM; 24h |
Applications | 3,3-Dimethyl-1-butanol treatment inhibited secretion of IL-1β and IL-6 from LPS-stimulated macrophages in a dose-dependent manner. |
| Animal experiment [2]: | |
Animal models | Male CD1 mice |
Preparation Method | Eight-week-old male CD1 mice were raised under a 12-hour light/12-hour dark cycle, with free access to food and water. Forty mice were selected and fed either a normal diet (ND, control group) or a high-fat diet (WD). Additionally, 1.0% 3,3-Dimethyl-1-butanol was added to the drinking water for 8 weeks (n=10 in each group). After 8 weeks of baseline and high-fat diet feeding, cardiac function was evaluated. |
Dosage form | 1.0% in drinking water for 8 weeks; p.o. |
Applications | 3,3-Dimethyl-1-butanol treatment significantly prevented the impairment of cardiac contraction and relaxation function in mice caused by a high-fat diet. |
References: | |
| Cas No. | 624-95-3 | SDF | |
| Synonyms | DMB; Neohexanol | ||
| Formula | C6H14O | M.Wt | 102.17 |
| Solubility | DMSO: 100 mg/mL (978.76 mM; solubilized by ultrasound); H2O: 10.5 mg/mL (102.77 mM; solubilized by ultrasound) | Storage | Store at RT |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 9.7876 mL | 48.938 mL | 97.8761 mL |
| 5 mM | 1.9575 mL | 9.7876 mL | 19.5752 mL |
| 10 mM | 978.8 μL | 4.8938 mL | 9.7876 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >99.50% Appearance: A liquid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)