PMSF (Synonyms: Benzylsulfonyl fluoride, NSC 88499, PMSF) |
Catalog No.GC10477
One-Click Copy Product Info
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PMSF is an irreversible serine protease inhibitor that is often added to the lysis buffer before cell or tissue lysis to prevent protein degradation.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 329-98-6
Sample solution is provided at 25 µL, 10mM.
PMSF is an irreversible serine protease inhibitor that is often added to the lysis buffer before cell or tissue lysis to prevent protein degradation[1,2]. PMSF can effectively inhibit chymotrypsin, trypsin, thrombin, etc. The principle of action of PMSF is that it can specifically recognize and sulfonate the serine residue active site that determines enzyme activity. PMSF is also an inhibitor of fatty acid amide hydrolase (FAAH), cysteine proteases (such as papain, the inhibitory activity of which is reversed by DTT), and acetylcholinesterase of mammalian origin[3]. The inhibitory effect of PMSF on papain can be reversed by DTT (Dithiothreitol).
The deactivation of PMSF increases with increasing pH and temperature. The half-lives of the inhibitors are approximately 110, 55, and 35 minutes at 25°C and pH 7.0, 7.5, and 8.0, respectively. At pH 8, 100μM PMSF is almost completely inactivated within 1 hour at 25°C or 22 hours at 4°C. PMSF stock solutions prepared using 100% isopropyl alcohol are stable for months or longer at 25°C. Within 1 week at 25°C and pH 7.0, chymotrypsin inhibited by PMSF did not reactivate[4].
In animal experiments, PMSF had significant analgesic effects at doses of 120 and 300 mg/kg. Sub-analgesic doses (60 mg/kg, i.p.) of PMSF can reduce tolerance resulting from acute and chronic administration of opioids[3].
PMSF can be used alone or often mixed with other protease inhibitors such as aprotinin A or phosphatase inhibitors such as sodium orthovanadate.
PMSF is poorly soluble in water and has poor stability in aqueous solutions. It is recommended to add it to lysis buffer or other aqueous solutions before use. PMSF powder can be dissolved in methanol, ethanol or isopropyl alcohol to prepare a storage solution. The recommended concentration is 100-200mM. The effective working concentration of PMSF is 0.1-1mM.
| Animal experiment [1]: | |
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Animal models |
Adult male mice |
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Preparation Method |
This assay measures analgesic activity. All animals were placed on a 55 ± 1 °C hot plate, and the latency time to either jumping or licking was recorded. The cut-off time was set as 15 s to protect mice from any damage. The antinociceptive effects of a single dose of morphine (10 mg/kg s.c.) and PMSF (60, 120, and 300 mg/kg) were determined. |
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Dosage form |
60, 120, and 300 mg/kg, i.p. |
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Applications |
The results suggest that the administration of the PMSF doses (120 and 300 mg/kg, i.p.) led to significant changes in the analgesic latency time, while administration with PMSF (60 mg/kg i.p.) had no significant difference. |
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References: [1]: Akbarabadi E A, Vardanjani H R, Molavinia S, et al. PMSF Attenuates Morphine Antinociceptive Tolerance and Dependence in Mice: Its Association with the Oxidative Stress Suppression[J]. Iranian Journal of Pharmaceutical Research: IJPR, 2021, 20(3): 300. |
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| Cas No. | 329-98-6 | SDF | |
| Synonyms | Benzylsulfonyl fluoride, NSC 88499, PMSF | ||
| Chemical Name | phenylmethanesulfonyl fluoride | ||
| Canonical SMILES | C1=CC=C(C=C1)CS(=O)(=O)F | ||
| Formula | C7H7FO2S | M.Wt | 174.2 |
| Solubility | ≥ 17.4mg/mL in DMSO | Storage | Store at RT |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 5.7405 mL | 28.7026 mL | 57.4053 mL |
| 5 mM | 1.1481 mL | 5.7405 mL | 11.4811 mL |
| 10 mM | 574.1 μL | 2.8703 mL | 5.7405 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
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