Acetylcysteine (Synonyms: N-acetylcysteine; N-acetyl-L-cysteine; NAC; Acetadote) |
Catalog No.GC11786
One-Click Copy Product Info
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Acetylcysteine is an N-acetyl derivative of L-cysteine and a glutathione precursor with mucolytic activity.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 616-91-1
Sample solution is provided at 25 µL, 10mM.
Acetylcysteine is an N-acetyl derivative of L-cysteine and a glutathione precursor with mucolytic activity. Acetylcysteine breaks disulfide bonds in mucoproteins through its free sulfhydryl group to reduce sputum viscosity. Acetylcysteine acts as a glutathione precursor to increase intracellular glutathione levels and scavenge reactive oxygen species. Acetylcysteine replenishes glutathione to neutralize toxic metabolites in acetaminophen poisoning. Acetylcysteine can be used in studies related to acetaminophen poisoning, chronic obstructive pulmonary disease, idiopathic pulmonary fibrosis, and acute lung injury[1-4].
In vitro, co-treatment of PC-9/GR cells with 5mM Acetylcysteine and 2µM gefitinib for 48 hours reduced the half-maximal inhibitory concentration value of gefitinib, decreased cell invasion and migration ability, and increased the proportion of apoptotic cells[5]. Pretreatment of MDA-MB468 human breast cancer cells with 1mM Acetylcysteine for 2 hours, followed by treatment with 25-500µM cadmium chloride for 48 hours, reversed the cadmium chloride-induced decrease in cell viability, inhibited cadmium chloride-induced DNA ladder fragmentation, and eliminated nuclear condensation[6]. Pretreatment of C6 astrocyte cells with 500µM Acetylcysteine for 1 hour, followed by treatment with 20mM monosodium glutamate for 12 or 24 hours, inhibited monosodium glutamate-induced reactive oxygen species generation and mitochondrial membrane potential loss, and prevented intracellular reduced glutathione depletion[7].
In vivo, C57BL/6 mice and 129S1/SvImJ mice received 0.5mg/kg or 1.0mg/kg Warfarin in drinking water combined with 30mg/kg Acetylcysteine by oral gavage for 23 weeks, starting 3 weeks after 5/6 nephrectomy. Acetylcysteine reduced serum creatinine levels, prevented Warfarin-induced systolic blood pressure elevation, and decreased renal tubulointerstitial fibrosis area[8]. Neonatal maternal separation (NMS) model Sprague-Dawley rats received intraperitoneal injections of 50mg/kg Acetylcysteine for 20 days. Acetylcysteine improved the prolonged immobility time in the tail suspension test and forced swim test in NMS rats, and improved the reduced sucrose preference in both male and female NMS rats[9]. BALB/c mice received intraperitoneal injections of 150mg/kg Acetylcysteine for 2 weeks. Acetylcysteine reduced testicular MDA levels, restored testicular and seminal vesicle weights and organ coefficients, reduced the magnitude of decrease in cauda epididymis sperm count, and improved sperm head deformity and immature nuclear protein abnormalities[10].
References:
[1] Moldéus P, Cotgreave IA. N-acetylcysteine. Methods Enzymol. 1994;234:482-92.
[2] Heard K, Green J. Acetylcysteine therapy for acetaminophen poisoning. Curr Pharm Biotechnol. 2012 Aug;13(10):1917-23.
[3] Ooi SL, Green R, Pak SC. N-Acetylcysteine for the Treatment of Psychiatric Disorders: A Review of Current Evidence. Biomed Res Int. 2018 Oct 22;2018:2469486.
[4] Singh J, Phogat A, Malik V. N-acetylcysteine: a potential therapeutic agent against toxicity of pesticides. Mol Biol Rep. 2025 Jun 3;52(1):539.
[5] Li J, Wang XH, Hu J, et al. Combined treatment with N-acetylcysteine and gefitinib overcomes drug resistance to gefitinib in NSCLC cell line. Cancer Med. 2020 Feb;9(4):1495-1502.
[6] Panjehpour M, Alaie SH. N-acetylcysteine prevents cadmium-induced apoptosis in human breast cancer MDA-MB468 cell line. Acta Medica Iranica. 2014;52(1):33-38.
[7] Park E, Yu KH, Kim DK, et al. Protective effects of N-acetylcysteine against monosodium glutamate-induced astrocytic cell death. Food Chem Toxicol. 2014;67:1-9.
[8] Medipally A, Xiao M, Satoskar AA, et al. N-acetylcysteine ameliorates hematuria-associated tubulointerstitial injury in 5/6 nephrectomy mice. Physiol Rep. 2023;11(13):e15767.
[9] Kim HB, Kim YJ, Lee YJ, et al. N-Acetylcysteine Alleviates Depressive-Like Behaviors in Adolescent EAAC1-/- Mice and Early Life Stress Model Rats. Int J Biol Sci. 2024;20(14):5450-5473.
[10] Wei Y, Geng W, Zhang T, et al. N-acetylcysteine rescues meiotic arrest during spermatogenesis in mice exposed to BDE-209. Environ Sci Pollut Res. 2023;30(20):50952-50968.
| Cell experiment [1]: | |
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Cell lines |
MDA-MB468 cells (human estrogen receptor-negative breast cancer cell line) |
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Preparation Method |
MDA-MB468 cells were cultured in RPMI-1640 medium supplemented with 10% heat-inactivated fetal bovine serum, 100U/mL penicillin and 100μg/mL streptomycin at 37°C, 5% CO2. Cells were pre-incubated with 1mM Acetylcysteine for 2h, then treated with 25,-500μM CdCl₂ for 48h. After treatment, cell viability, apoptosis, DNA fragmentation was measured. |
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Reaction Conditions |
1mM; 2h pretreatment |
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Applications |
Acetylcysteine reversed CdCl₂-induced reduction in MDA-MB468 cell viability. Acetylcysteine eliminated CdCl2-induced nuclear condensation, chromatin fragmentation and apoptotic bodies. Acetylcysteine prevented CdCl2-induced internucleosomal DNA ladder fragmentation. |
| Animal experiment [2]: | |
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Animal models |
7-week-old male BALB/c mice (22-25g) |
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Preparation Method |
After 1 week acclimation, mice were randomized into four groups (vehicle DMSO, BDE-209 80mg/kg, Acetylcysteine 150mg/kg, Acetylcysteine 150mg/kg + BDE-209 80mg/kg). Starting at 8 weeks of age, each mouse received 0.2mL i.p. every morning for 2 weeks; Acetylcysteine was given 2h before BDE-209 in the combination group. About 8.6 days after last administration, mice were anesthetized with chloroform and sacrificed; left testis was fixed in Bouin's/10% formalin for histology, right testis snap-frozen for MDA/GSH-Px assay, Western blot, RT-qPCR, flow cytometry, chromosome spread immunofluorescence, and epididymal sperm count/morphology. |
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Dosage form |
150mg/kg; i.p.; daily for 2 weeks |
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Applications |
Acetylcysteine lowered testicular MDA level elevated by BDE-209. Acetylcysteine restored testicular weight and seminal vesicle weight reduced by BDE-209. Acetylcysteine decreased the percentage of abnormal pachytene spermatocytes and increased round spermatid percentage in BDE-209-exposed seminiferous tubules. Acetylcysteine recovered Stra8, H1T, Spo11, Sycp3, γ-H2AX, DMC1, RAD51, MLH1 protein and mRNA levels reduced by BDE-209. Acetylcysteine attenuated pachytene-stage H3K4me3 focus number increased by BDE-209. Acetylcysteine improved cauda epididymis sperm count and reduced sperm head abnormality and immature nuclear protein rate induced by BDE-209. |
References: [1] Panjehpour M, Alaie SH. N-acetylcysteine prevents cadmium-induced apoptosis in human breast cancer MDA-MB468 cell line. Acta Med Iran. 2014;52(1):33-38. [2] Wei Y, Geng W, Zhang T, et al. N-acetylcysteine rescues meiotic arrest during spermatogenesis in mice exposed to BDE-209. Environ Sci Pollut Res. 2023;30:50952-50968. | |
| Cas No. | 616-91-1 | SDF | |
| Synonyms | N-acetylcysteine; N-acetyl-L-cysteine; NAC; Acetadote | ||
| Chemical Name | (2R)-2-acetamido-3-sulfanylpropanoic acid | ||
| Canonical SMILES | CC(=O)NC(CS)C(=O)O | ||
| Formula | C5H9NO3S | M.Wt | 163.19 |
| Solubility | DMF: 50 mg/mL,DMSO: 50 mg/mL,Ethanol: 50 mg/mL,PBS (pH 7.2): 30 mg/mL | Storage | 4°C, protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 6.1278 mL | 30.6391 mL | 61.2783 mL |
| 5 mM | 1.2256 mL | 6.1278 mL | 12.2557 mL |
| 10 mM | 612.8 μL | 3.0639 mL | 6.1278 mL |
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
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- Purity: >99.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 26 reference(s) in Google Scholar.)