FITC-Dextran (MW 10000) |
| Catalog No.: GC36048 |
FITC-Dextran is a fluorescent probe for fluorescein isothiocyanate (FITC) dextran (Ex=495 nm; Em=525 nm).
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.:60842-46-8
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
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Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
| Cell experiment [1]: | |
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Cell lines |
Human fibroblast |
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Preparation Method |
Aspire the cell culture media from the cells and add 1 ml of FITC-Dextran containing medium and incubate for 3 days at 37 °C with 5% of CO2 in air. |
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Reaction Conditions |
1mL 0.1 mg/ml FITC-Dextran for 3 days at 37 °C |
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Applications |
When analyse the lysosomal pH in cultured cells using the fluorescent probe fluorescein isothiocyanate (FITC)-dextran together with a dual-emission ratiometric technique suitable for flow cytometry. Fluorescence-labeled dextran is endocytosed and accumulated in the lysosomal compartment. FITC shows a pH-dependent variation in fluorescence when analyzed at maximum emission wavelength and no variation when analyzing at the isosbestic point, thereby the ratio can be used to determine the lysosomal pH. |
| Animal experiment [2]: | |
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Animal models |
Specific-pathogen-free male C3H/HeOuJ mice |
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Preparation Method |
The 12-week-old male mice were fasted for 6 h and 4-kDa FITC-Dextran or 70-kDa FITC-Dextran was applied orally at a single dose. Three mice each were anaesthetized after 30 min, 1, 1.5, 2 and 8 h; another three mice were anaesthetized after 15 and 45 min and blood from the retrobulbar capillary plexus was sampled into heparinized tubes for 4-kDa FITC-Dextran analyses. Plasma was obtained after centrifugation at 2000 g for 5 min. |
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Dosage form |
600 mg/kg 4-kDa FITC dextran;(100 µL, 5 mg/mL PBS)70-kDa FITC-Dextran for 30min-8h |
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Applications |
After 6 h of fasting in our C3H/HeOu mice, plasma concentrations of 4-kDa FITC-Dextran peaked at 45 min after oral administration. |
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References: [1]: Eriksson I, Öllinger K, Appelqvist H. Analysis of Lysosomal pH by Flow Cytometry Using FITC-Dextran Loaded Cells. Methods Mol Biol. 2017;1594:179-189. doi: 10.1007/978-1-4939-6934-0_11. PMID: 28456983. |
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FITC-Dextran is a fluorescent probe for fluorescein isothiocyanate (FITC) dextran (Ex=495 nm; Em=525 nm). In cell permeability studies, FITC-Dextran can assess BBB permeability and BBB disruption [1,2].
When analyse the lysosomal pH in cultured cells using the fluorescent probe fluorescein isothiocyanate (FITC)-dextran together with a dual-emission ratiometric technique suitable for flow cytometry. Fluorescence-labeled dextran is endocytosed and accumulated in the lysosomal compartment. FITC shows a pH-dependent variation in fluorescence when analyzed at maximum emission wavelength and no variation when analyzing at the isosbestic point, thereby the ratio can be used to determine the lysosomal pH[2].Fitc-glucan increased intestinal epithelial paracellular permeability after 21 days of incubation[5]. When examined their expression and FITC-Dextran uptake by various human DC preparations. In contrast to immature Mo-DC, the FITC-dextran uptake by LC was not inhibited effectively by mannose, an inhibitor for MMR-mediated FITC-dextran uptake[7].
After 6 h of fasting in our C3H/HeOu mice, plasma concentrations of 4-kDa FITC-Dextran peaked at 45 min after oral administration[3]. The fluorescence of small vessels and neovascular tufts could be observed clearly following RO injection of 0.05 ml of 25 mg/ml or 50 mg/ml FITC-dextran. No visible damage to tissues adjacent to the injection site was discovered. Vitreous blood flow was gradually reduced from P0 to P5 and eventually disappeared in P17 OIR mice, as demonstrated by FITC-dextran perfusion. The retinal NV areas assessed by isolectin B4 were larger than those assessed by FITC-dextran, but the retinal avascular areas were smaller[4]. When measured fluorescence recovery after photobleaching (FRAP) of fluorescein isothiocyanate (FITC)-labelled 10 and 250 kDa dextran (FITC-Dextran) in isolated rat descending colonic crypts.FRAP of either 10 or 250 kDa FITC-Dextran in crypt lumens was almost complete within 2-3 min. In the presence of amiloride (0.1 mM), or in the absence of Na+, the rate of FITC-Dextran uptake into the crypt lumens was reduced by 70-80 %[6].
References:
[1]: Natarajan R, Northrop N, et,al. Fluorescein Isothiocyanate (FITC)-Dextran Extravasation as a Measure of Blood-Brain Barrier Permeability. Curr Protoc Neurosci. 2017 Apr 10;79:9.58.1-9.58.15. doi: 10.1002/cpns.25. PMID: 28398646; PMCID: PMC5470084.
[2]: Eriksson I, Öllinger K, et,al.Analysis of Lysosomal pH by Flow Cytometry Using FITC-Dextran Loaded Cells. Methods Mol Biol. 2017;1594:179-189. doi: 10.1007/978-1-4939-6934-0_11. PMID: 28456983.
[3]: Woting A, Blaut M. Small Intestinal Permeability and Gut-Transit Time Determined with Low and High Molecular Weight Fluorescein Isothiocyanate-Dextrans in C3H Mice. Nutrients. 2018 May 28;10(6):685. doi: 10.3390/nu10060685. PMID: 29843428; PMCID: PMC6024777.
[4]: Li J, Wu Y, et,al. Retro-orbital injection of FITC-dextran combined with isolectin B4 in assessing the retinal neovascularization defect. BMC Ophthalmol. 2021 May 11;21(1):208. doi: 10.1186/s12886-021-01969-5. PMID: 33975571; PMCID: PMC8112026.
[5]: Sun X, Yang Q, et,al.AMPK improves gut epithelial differentiation and barrier function via regulating Cdx2 expression. Cell Death Differ. 2017 May;24(5):819-831. doi: 10.1038/cdd.2017.14. Epub 2017 Feb 24. PMID: 28234358; PMCID: PMC5423107.
[6]: Thiagarajah JR, Pedley KC, et,al. Evidence of amiloride-sensitive fluid absorption in rat descending colonic crypts from fluorescence recovery of FITC-labelled dextran after photobleaching. J Physiol. 2001 Oct 15;536(Pt 2):541-53. doi: 10.1111/j.1469-7793.2001.0541c.xd. PMID: 11600688; PMCID: PMC2278881.
[7]: Kato M, Neil TK,et,al. Expression of multilectin receptors and comparative FITC-dextran uptake by human dendritic cells. Int Immunol. 2000 Nov;12(11):1511-9. doi: 10.1093/intimm/12.11.1511. PMID: 11058570.
| Cas No. | 60842-46-8 | SDF | |
| Canonical SMILES | [FITC-Dextran] | ||
| Formula | M.Wt | 10000.00(Average) | |
| Solubility | Water: 50 mg/mL | Storage | 4°C,sealed storage,away from moisture and light |
| General tips | For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months. | ||
| Shipping Condition | Evaluation sample solution : ship with blue ice All other available size: ship with RT , or blue ice upon request |
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Weak Electric Current Treatment to Artificially Enhance Vascular Permeability in Embryonated Chicken Eggs
Biol Pharm Bull 2020;43(11):1729-1734.PMID:33132318DOI:10.1248/bpb.b20-00423.
Technologies that overcome the barrier presented by vascular endothelial cells are needed to facilitate targeted delivery of drugs into tissue parenchyma by intravenous administration. We previously reported that weak electric current treatment (ET: 0.3-0.5 mA/cm2) applied onto skin tissue in a transdermal drug delivery technique termed iontophoresis induces cleavage of intercellular junctions that results in permeation of macromolecules such as small interfering RNA and cytosine-phosphate-guanine (CpG) oligonucleotide through the intercellular space. Based on these findings, we hypothesized that application of ET to blood vessels could promote cleavage of intercellular junctions that artificially induces increase in vascular permeability to enhance extravasation of drugs from the vessels into target tissue parenchyma. Here we investigated the effect of ET (0.34 mA/cm2) on vascular permeability using embryonated chicken eggs, which have blood vessels in the chorioallantoic membrane (CAM), as an animal model. ET onto the CAM of the eggs significantly increased extravasation of intravenously injected calcein (M.W. 622.6), a low molecular weight compound model, and the macromolecule fluorescein isothiocyanate (FITC)-dextran (M.W. 10000). ET-mediated promotion of penetration of FITC-Dextran through vascular endothelial cells was also observed in transwell permeability assay using monolayer of human umbilical vein endothelial cells without induction of obvious cellular damage. Confocal microscopy detected remarkable fluorescence derived from injected FITC-Dextran in blood vessel walls. These results in embryonated chicken eggs suggest that ET onto blood vessels could artificially enhance vascular permeability to facilitate extravasation of macromolecules from blood vessels.
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