FITC-Dextran (MW 10000) |
Catalog No.GC36048
One-Click Copy Product Info
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FITC-Dextran (MW 10000) is a fluorescent probe (Ex=495nm; Em=525nm) that serves as a measure of blood-brain barrier permeability.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 60842-46-8
Sample solution is provided at 25 µL, 10mM.
FITC-Dextran (MW 10000) is a fluorescent probe (Ex=495nm; Em=525nm) that serves as a measure of blood-brain barrier permeability[1]. Perfusion of FITC-Dextran through the transpiration stream into isolated sunflower leaves allows the measurement of intercellular pH values and pH gradients between different cell types within the sunflower leaves[2]. FITC-Dextran has been widely used as a model hydrophilic compound to study the effects of process parameters on the morphology of microspheres and the release of FITC-Dextran from Poly(lactic-co-glycolic acid) microspheres[3]. FITC-Dextran can be used as a marker to reveal the cellular damage induced by heat shock and to study the early as well as the late stages of apoptosis[4]. FITC-Dextran has been widely used as a tracer in studies of microcirculation and permeability, allowing for the tracking of specific microcirculatory regions within the body[5].
References:
[1] Natarajan R, Northrop N, Yamamoto B. Fluorescein isothiocyanate (FITC)‐dextran extravasation as a measure of blood‐brain barrier permeability[J]. Current protocols in neuroscience, 2017, 79(1): 9.58. 1-9.58. 15.
[2] Hoffmann B, Kosegarten H. FITC‐dextran for measuring apoplast pH and apoplastic pH gradients between various cell types in sunflower leaves[J]. Physiologia Plantarum, 1995, 95(3): 327-335.
[3] Mao S, Xu J, Cai C, et al. Effect of WOW process parameters on morphology and burst release of FITC-dextran loaded PLGA microspheres[J]. International journal of pharmaceutics, 2007, 334(1-2): 137-148.
[4] Moumaris M, Rajoely B, Abuaf N. Fluorescein isothiocyanate-dextran can track apoptosis and necrosis induced by heat shock of peripheral blood mononuclear cells and HeLa cells[J]. Open Biological Sciences Journal, 2015, 1(1): 7-15.
[5] Thorball N. FITC-dextran tracers in microcirculatory and permeability studies using combined fluorescence stereo microscopy, fluorescence light microscopy and electron microscopy[J]. Histochemistry, 1981, 71(2): 209-233.
Evaluation of Apoptosis in Cells using FITC-Dextran
This protocol only provides a guideline and should be modified according to your specific needs
1. Stock and dilute solution preparation for staining of cells
(1) Prepare FITC-Dextran stock solution (1mM) by dissolving FITC-Dextran powder in DMSO.
(2) Dilute the FITC-Dextran stock solution with PBS to a final 2µM concentration. NOTE: The working solution must be prepared fresh on the day of the experiment.
2. Cell Preparation
Seed cultured cells in a 6-well plate or a 60-mm culture dish, depending on the scale of the experiment. If the cells tend to detach easily, precoat the plate or dish with poly-l/d-lysine to enhance cell adhesion.
3. Labeling of cells and analysis
(1) To induce apoptosis, cells were heated to 43.5°C for 1 hour and then incubated at 37°C for 8h.
(2) Wash the cells twice with PBS.
(3) Add FITC-Dextran in PBS at a final concentration of 2μM, and incubate at 4°C for 30 minutes in the dark with gentle agitation.
(4) Collect cells into a 1.5ml tube and centrifuge at 500g for 10 minutes and discard the supernatant.
(5) Resuspend cells in culture medium and analyze using flow cytometry or fluorescence microscopy.
4. Experimental Precautions
(1) Dissolve the FITC-Dextran in PBS immediately before use, and ensure that the FITC-Dextran solution is adequately protected from light.
(2) This protocol and all reagents are for scientific research use only.
References:
[1] Moumaris M, Rajoely B, Abuaf N. Fluorescein isothiocyanate-dextran can track apoptosis and necrosis induced by heat shock of peripheral blood mononuclear cells and HeLa cells[J]. Open Biological Sciences Journal, 2015, 1(1): 7-15.
| Cas No. | 60842-46-8 | SDF | |
| Canonical SMILES | [FITC-Dextran] | ||
| Formula | M.Wt | 10000.00(Average) | |
| Solubility | Water: 50 mg/mL | Storage | 4°C,sealed storage,away from moisture and light |
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 100 μL | 500 μL | 1 mL |
| 5 mM | 20 μL | 100 μL | 200 μL |
| 10 mM | 10 μL | 50 μL | 100 μL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 3 reference(s) in Google Scholar.)