Morusin (Synonyms: Mulberrochromene, NSC 649220) |
Catalog No.GN10436
One-Click Copy Product Info
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Morusin is a significant prenylated flavone found in Morus alba (Moraceae) root cortex, which exhibits significant anti-tumor activity in the treatment of prostate cancer.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 62596-29-6
Sample solution is provided at 25 µL, 10mM.
Morusin is a significant prenylated flavone found in Morus alba (Moraceae) root cortex, which exhibits significant anti-tumor activity in the treatment of prostate cancer[1]. Prenylated flavonoids, a unique class of flavonoids which combine a flavonoid skeleton and a lipophilic prenyl side-chain, possess great potential biological activities including cytotoxicity, anti-inflammation, anti-Alzheimer, anti-diabetes and other effects[2]. Morusin as a candidate for the new efficacious mucoregualtors for inflammatory pulmonary diseases[3].
In vitro, treatment of PC3 cells with morusin (0-10μM) for 48h significantly inhibited cell viability in a time- and concentration-dependent manner and reduced levels of inflammatory cytokines[4]. In NCI-H292 cells deprived of serum for 24h, morusin (1-100μM) administered for 30minutes significantly suppressed the production of mucin 5AC (MUC5AC), thereby controlling various pulmonary inflammatory responses caused by excessive mucus secretion[3]. In cancer cell lines (MKN45 and SGC7901) treated with moracin (1-5mg/L) for 72h, moracin effectively inhibited cancer cell growth and proliferation in a dose-dependent manner[5].
In vivo, administration of morusin (5mg/kg/5days) via intragastric administration for 28days in ovariectomized rats, morusin alleviated estrogen deficiency-induced osteoporosis and reduced the expression of RUNX2 and β-catenin in femoral bone tissue[6]. In a destabilization of the medial meniscus osteoarthritis (DMM) mouse model, administration of morusin (5mg/kg/2days) via intragastric administration for 56days, morusin mitigated cartilage erosion and destruction while decreasing the expression levels of associated inflammatory factors and proteins[7].
References:
[1] Dirir A M, Daou M, Yousef A F, et al. A review of alpha-glucosidase inhibitors from plants as potential candidates for the treatment of type-2 diabetes[J]. Phytochem Rev, 2022, 21(4): 1049-1079.
[2] Shi S, Li J, Zhao X, et al. A comprehensive review: Biological activity, modification and synthetic methodologies of prenylated flavonoids[J]. Phytochemistry, 2021, 191: 112895.
[3] Lee H J, Ryu J, Park S H, et al. Effects of Morus alba L. and Natural Products Including Morusin on In Vivo Secretion and In Vitro Production of Airway MUC5AC Mucin[J]. Tuberc Respir Dis (Seoul), 2014, 77(2): 65-72.
[4] Fadil S A, Albadawi D a I, Alshali K Z, et al. Modulation of inflammatory mediators underlies the antitumor effect of the combination of morusin and docetaxel on prostate cancer cells[J]. Biomed Pharmacother, 2024, 180: 117572.
[5] Wang F, Zhang D, Mao J, et al. Morusin inhibits cell proliferation and tumor growth by down-regulating c-Myc in human gastric cancer[J]. Oncotarget, 2017, 8(34): 57187-57200.
[6] Chen M, Han H, Zhou S, et al. Morusin induces osteogenic differentiation of bone marrow mesenchymal stem cells by canonical Wnt/β-catenin pathway and prevents bone loss in an ovariectomized rat model[J]. Stem Cell Res Ther, 2021, 12(1): 173.
[7] Jia Y, He W, Zhang H, et al. Morusin Ameliorates IL-1β-Induced Chondrocyte Inflammation and Osteoarthritis via NF-κB Signal Pathway[J]. Drug Des Devel Ther, 2020, 14: 1227-1240.
| Cell experiment [1]: | |
Cell lines | PC3 (Human Prostate Cancer Cells) |
Preparation Method | In 96-well culture plates, PC3 cells were planted at a density of 4×104 cells/well. They were cultured for 24h to allow them to stick to the plates before being subjected to the medications. Serial dilutions of morusin (0, 1.25, 2.5, 5, 10µM) was applied to the cells to measure the inhibitory concentration 50. Following a 48h treatment period, the cells were immobilized by applying 10% trichloroacetic acid at a temperature of 4℃ for a duration of 1h. Afterwards, the cells were treated with Sulforhodamine-B (0.4%) for a duration of 30minutes, then rinsed with 1% acetic acid and allowed to dry in the air. The dye was eliminated by rinsing the plate with a solution of 10mM tris base at a pH of 10.5. Subsequently, the optical density of the wells was quantified at a wavelength of 570nm using an ELISA microplate reader. |
Reaction Conditions | 0, 1.25, 2.5, 5, and 10μM; 48h |
Applications | Morusin significantly inhibited cell viability and reduced levels of inflammatory cytokines. |
| Animal experiment [2]: | |
Animal models | C57BL/6 mice (males and females) |
Preparation Method | Mice were randomly divided into three groups (Sham group, DMM group and morusin treated group), and were anesthetized, at which the mice knee joints were carefully, and aseptically, exposed with a scalpel, followed by resectioning of the medial meniscuses. The wound was later sutured carefully, and penicillin was injected intramuscularly for the following 3days. The mourusin-treated group was administered morusin, dissolved in saline (40mg/kg), intragastrically once every 2days for 8weeks; while mice in the Sham and DMM groups received 5% DMSO (dimethyl-sulfoxide) in saline once every 2days for 8weeks. |
Dosage form | 40mg/kg/2days for 56days; Intragastric administration |
Applications | Morusin mitigated cartilage erosion and destruction and decreased the expression levels of associated inflammatory factors and proteins. |
References: | |
| Cas No. | 62596-29-6 | SDF | |
| Synonyms | Mulberrochromene, NSC 649220 | ||
| Chemical Name | 2-(2,4-dihydroxyphenyl)-5-hydroxy-8,8-dimethyl-3-(3-methylbut-2-enyl)pyrano[2,3-h]chromen-4-one | ||
| Canonical SMILES | CC(=CCC1=C(OC2=C3C=CC(OC3=CC(=C2C1=O)O)(C)C)C4=C(C=C(C=C4)O)O)C | ||
| Formula | C25H24O6 | M.Wt | 420.47 |
| Solubility | ≥ 49.3mg/mL in DMSO | Storage | Store at -20°C, protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.3783 mL | 11.8915 mL | 23.7829 mL |
| 5 mM | 475.7 μL | 2.3783 mL | 4.7566 mL |
| 10 mM | 237.8 μL | 1.1891 mL | 2.3783 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
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- Purity: >98.50% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)