|25(R)-27-hydroxy Cholesterol Catalog No.GC13887|
Sample solution is provided at 25 µL, 10mM.
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Stock solutions of 27-Hydroxycholesterol are prepared in 100% ethanol and stored at −80°C. 27-Hydroxycholesterol stock solution is dissolved in appropriate volumes of media to prepare the working solutions of 1 μM.
Proliferation assays are conducted on black 96 well plates using a commercial kit which quantifies cell number using DNA content and membrane integrity. LNCaP and PC3 cells are treated with 1 µM 27-Hydroxycholesterol for 48 hours.
. DuSell CD, et al. 27-hydroxycholesterol is an endogenous selective estrogen receptor modulator. Mol Endocrinol. 2008 Jan;22(1):65-77.
|Solubility||≤20mg/ml in ethanol;0.1mg/ml in DMSO;2mg/ml in dimethyl formamide||Storage||Store at -20°C|
|General tips||For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.|
|Shipping Condition||Evaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request
25(R)-27-hydroxy Cholesterol ((25R)-26-hydroxy cholesterol) is the naturally occurring isomer of the endogenous the endogenous 27-hydroxycholesterol. And approximately 80% is the naturally occurring (25R) isomer . 27-hydroxycholesterol is an endogenous ligand of liver X receptor with EC50 values of 85 and 71 nM for LXRα and LXRβ, respectively .
The liver x receptors (LXRα and LXRβ) are nuclear hormone receptors that are important regulators of cholesterol and fatty acid metabolism .
27-hydroxycholesterol is synthesized by a mitochondrial P-450 enzyme that appears to be widely distributed in tissues . 27-hydroxycholestero1 can inhibit both cholesterol synthesis and low density lipoprotein (LDL) receptor activity . In cholesterol-loaded human macrophage cells, 27-hydroxycholestero1 is an endogenous ligand for LXRs with Kd values of 85 and 71 nM for LXRα and LXRβ, respectively . In human fibroblasts, low density lipoprotein cholesterol is converted to 27-hydroxycholesterol, which was sufficient to reduce the activity of HMG-CoA reductase .
. Javitt NB. 26-Hydroxycholesterol: synthesis, metabolism, and biologic activities. J Lipid Res. 1990 Sep;31(9):1527-33.
. Fu X, Menke JG, Chen Y, et al. 27-hydroxycholesterol is an endogenous ligand for liver X receptor in cholesterol-loaded cells. J Biol Chem. 2001 Oct 19;276(42):38378-87.
. Zhang Y, Mangelsdorf DJ. LuXuRies of lipid homeostasis: the unity of nuclear hormone receptors, transcription regulation, and cholesterol sensing. Mol Interv. 2002 Apr;2(2):78-87.
. Axelson M, Larsson O. Low density lipoprotein (LDL) cholesterol is converted to 27-hydroxycholesterol in human fibroblasts. Evidence that 27-hydroxycholesterol can be an important intracellular mediator between LDL and the suppression of cholesterol production. J Biol Chem. 1995 Jun 23;270(25):15102-10.