FT895 |
Catalog No.GC65206
One-Click Copy Product Info
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FT895 is an effective selective inhibitor of histone deacetylase 11 (HDAC11), with an IC50 value of 3nM .
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 2225728-57-2
Sample solution is provided at 25 µL, 10mM.
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FT895 is an effective selective inhibitor of histone deacetylase 11 (HDAC11), with an IC50 value of 3nM [1]. HDAC11 is a novel and unique member of the histone deacetylase family, which is highly expressed in specific tissues and certain cancer cell lines [2]. HDAC11 possesses both histone and non-histone[3] deacetylase activities and can participate in cellular activities such as chromatin remodeling and metabolic signaling pathways by regulating the acetylation state of the cell[3]. As a selective inhibitor of HDAC11, FT895 has demonstrated excellent cellular activity and pharmacokinetic properties[4], making FT895 an effective tool for studying the biological mechanisms of HDAC11. Additionally, FT895 holds promising applications in the treatment of cancer and inflammatory diseases.
In vitro, treatment with FT895 (10μM) for 24 hours resulted in a significant reduction of migration ability in A549 and H1650 cells[5]. Treatment with FT895 (10μM) for 1 hour resulted in a significant improvement of bactericidal efficiency in BMDM (Bone Marrow Derived Macrophage) cells[6].
In vivo, intraperitoneal injection of FT895 (10mg/kg/day) for 5 days in C57BL/6J mice promoted the expression of the mitochondrial inner membrane transporter UCP1 (Uncoupling protein 1) in adipose tissue through the inhibition of HDAC11, ultimately resulting in an increase in core body temperature in mice [7]. Intraperitoneal injection of FT895 (50mg/kg/day) for 7 days in C57BL/6 mice led to the inhibition of HDAC11 activity, which upregulated the expression of the anti-inflammatory cytokine IL-10 (Interleukin-10), thereby promoting anti-inflammatory responses and reducing the replication of enteric virus EV71 in the mice[8].
References:
[1] Huang, P.-Y., et al., FT895 Impairs Mitochondrial Function in Malignant Peripheral Nerve Sheath Tumor Cells. International Journal of Molecular Sciences, 2023. 25(1).
[2] Lin Gao, M.A.C., Fred Asselbergs, Peter Atadja, Cloning and Functional Characterization of HDAC11, a Novel Member of the Human Histone Deacetylase Family. Journal of Biological Chemistry, 2002. 277(28): p. 25748-25755.
[3] Joshi, P., et al., The functional interactome landscape of the human histone deacetylase family. Molecular Systems Biology, 2013. 9(1): p. 672.
[4] Matthew W. Martin, J.Y.L., David R. Lancia, Pui Yee Ng, Bingsong Han, Jennifer R. Thomason, Maureen S. Lynes, C. Gary Marshall, Chiara Conti, Alan Collis, Monica Alvarez Morales, Kshama Doshi, Aleksandra Rudnitskaya, Lili Yao, Xiaozhang Zheng, Discovery of novel N-hydroxy-2-arylisoindoline-4-carboxamides as potent and selective inhibitors of HDAC11. Bioorganic & Medicinal Chemistry Letters, 2018. 28(12): p. 2143-2147.
[5] Bora-Singhal, N.e.a., Novel HDAC11 inhibitors suppress lung adenocarcinoma stem cell self-renewal and overcome drug resistance by suppressing Sox2. Scientific reports, 2020. 10,1 4722.
[6] Wu, H.e.a., HDAC11 negatively regulates antifungal immunity by inhibiting Nos2 expression via binding with transcriptional repressor STAT3. Redox biology, 2022. 56 (2022): 102461.
[7] Robinson EL, B.R., Major JL, Bergman BC, Matsuda JL, McKinsey TA, HDAC11 inhibition triggers bimodal thermogenic pathways to circumvent adipocyte catecholamine resistance. J Clin Invest, 2023.
[8] Xie, H.e.a., Targeting HDAC11 activity by FT895 restricts EV71 replication. Virus research, 2023. vol. 330 (2023): 199108.
| Cell experiment [1]: | |
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Cell lines |
A549，H1650 |
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Preparation Method |
A549 and H1650 cells need to be cultured in 6-well plates until 90-95% confluent. Then a sterile 2µl pipette tip is used to create a scratch in each hole. Wash the cells with PBS, and then incubate the cells at 37°C for 24h in a medium supplemented with 10μM FT895. Finally, images of the wound healing process will be captured using a suitable imaging system for observation and analysis. |
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Reaction Conditions |
10 μM;24h |
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Applications |
Treatment with FT895 (10μM) for 24 hours resulted in a significant reduction of migration ability in A549 and H1650 cells. |
| Animal experiment [2]: | |
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Animal models |
C57BL/6J mice |
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Preparation Method |
A suitable number of 6-week-old C57BL/6 male mice should be prepared and placed in clean, individually ventilated cages. Then mice will be infected with 1 × 106 PFU Gluc-EV71 in 100 µl PBS for 7 days. Simultaneously, FT895 (50mg/kg/day) will also be intraperitoneally injected for 7 days. Finally, the virus copy number can be determined by qPCR. |
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Dosage form |
50mg/kg/day for 7 days; i.p. injection |
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Applications |
FT895 (10mg/kg/day) upregulates the expression of the anti-inflammatory factor IL-10 (Interleukin-10) in C57BL/6J mice, promotes anti-inflammatory responses, and reduces the replication of the enterovirus EV71 in mice. |
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References: |
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| Cas No. | 2225728-57-2 | SDF | |
| Formula | C16H15F3N4O2 | M.Wt | 352.31 |
| Solubility | DMSO : 125 mg/mL (354.80 mM; Need ultrasonic) | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.8384 mL | 14.192 mL | 28.3841 mL |
| 5 mM | 567.7 μL | 2.8384 mL | 5.6768 mL |
| 10 mM | 283.8 μL | 1.4192 mL | 2.8384 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)