BMPO (Synonyms: BocMPO) |
Catalog No.GC41397
One-Click Copy Product Info
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BMPO is a cell-permeable superior spin trap that captures OH and O2•−, forming stable BMPO/OH and •BMPO-OOH adducts.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 387334-31-8
Sample solution is provided at 25 µL, 10mM.
BMPO is a cell-permeable superior spin trap that captures OH and O2•−, forming stable BMPO/OH and •BMPO-OOH adducts[1]. Using BMPO, the conversion of BMPO-OOH to BMPO-OH in the presence of glutathione (GSH) can be detected[2]. BMPO has been widely used for detecting the superoxide produced by the nitric oxide synthase (NOS) in neurons[3].
References:
[1] Misak A, Brezova V, Grman M, et al. • BMPO-OOH Spin-Adduct as a Model for Study of Decomposition of Organic Hydroperoxides and the Effects of Sulfide/Selenite Derivatives. An EPR Spin-Trapping Approach[J]. Antioxidants, 2020, 9(10): 918.
[2] Ranade S S, Díaz D F Z, Meinke M C, et al. Advancing EPR spectroscopy with BMPO for UVA-induced radical detection in skin: Refining spin trapping and uncovering glutathione-dependent oxidative mechanisms[J]. Chemico-Biological Interactions, 2025: 111744.
[3] Zhao H, Joseph J, Zhang H, et al. Synthesis and biochemical applications of a solid cyclic nitrone spin trap: a relatively superior trap for detecting superoxide anions and glutathiyl radicals[J]. Free Radical Biology and Medicine, 2001, 31(5): 599-606.
Evaluation of hydroxyl radicals using BMPO
This protocol only provides a guideline and should be modified according to your specific needs
1. Stock and working solution preparation
(1) Prepare BMPO stock solution (100mM) by dissolving BMPO powder in DMSO, which was then aliquoted into appropriate volumes and stored at -20°C.
(2) Dilute the BMPO stock solution with phosphate buffer to a final 30mM working solution.
NOTE: The working solution must be prepared fresh on the day of the experiment.
2. Electron paramagnetic resonance (EPR) Study of the BMPO-Adducts
(1) BMPO-OOH was prepared by the addition of the saturated KO2/DMSO solution (10% v/v DMSO/final buffer) to the BMPO (30mM final concentration; 37°C) diluted in the phosphate buffer.
(2) The BMPO/KO2 sample was mixed for 10s and then the studied compounds, H2Sn, SeOn2− or H2Sn/SeOn2− mixtures, were added. The sample was mixed again for 5s and transferred to a standard cavity aqueous EPR flat. The first EPR spectrum was recorded 100s after the addition of the compounds. The sets of the individual EPR spectra of the BMPO spin-adducts were recorded as 15 sequential scans, each 42s, with a total acquisition time of 11min. Each experiment was repeated at least twice.
3. Experimental Precautions
(1) This protocol and all reagents are for scientific research use only.
(2) For your safety and health, please wear a laboratory coat and put on disposable gloves when operating.
References:
[1] Misak A, Brezova V, Grman M, et al. • BMPO-OOH Spin-Adduct as a Model for Study of Decomposition of Organic Hydroperoxides and the Effects of Sulfide/Selenite Derivatives. An EPR Spin-Trapping Approach[J]. Antioxidants, 2020, 9(10): 918.
| Cas No. | 387334-31-8 | SDF | |
| Synonyms | BocMPO | ||
| Chemical Name | 3,4-dihydro-2-methyl-1,1-dimethylethyl ester-2H-pyrrole-2-carboxylic acid-1-oxide | ||
| Canonical SMILES | [O-][N+]1=CCCC1(C)C(OC(C)(C)C)=O | ||
| Formula | C10H17NO3 | M.Wt | 199.2 |
| Solubility | 33mg/mL in ethanol, 25mg/mL in DMSO or in DMF, 10mg/mL in water | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 5.0201 mL | 25.1004 mL | 50.2008 mL |
| 5 mM | 1.004 mL | 5.0201 mL | 10.0402 mL |
| 10 mM | 502 μL | 2.51 mL | 5.0201 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 18 reference(s) in Google Scholar.)