BMPO (Synonyms: BocMPO) |
| Catalog No.GC41397 |
BMPO is a cyclic nitrone spin trap agent, it is a water-soluble white solid which makes BMPO purification easier than other spin trap agents.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 387334-31-8
Sample solution is provided at 25 µL, 10mM.
BMPO is a cyclic nitrone spin trap agent, it is a water-soluble white solid which makes BMPO purification easier than other spin trap agents. Even after prolonged storage at 220°C, there was no artificial signal formation from aqueous solutions containing BMPO (25–100 mM). BMPO offers several advantages over the existing spin traps in the detection and characterization of thiyl radicals, hydroxyl radicals, and superoxide anions in biological systems. One of the perceived advantages of BMPO is that the BMPO/•OOH adduct does not readily decay nonenzymatically to the BMPO/•OH adduct. [1]
The in vitro experiment took advantage of Rabbit aortic segments, which were treated with ADR and incubation with 0.1 M BMPO, a novel solid superoxide spin trap. Results showed that BMPO-hydroxyl radical adduct was detected in supernatants upon 10-min incubation of aortic segment with incremental doses of ADR.[2] Time-dependent changes in the ESR spectra of superoxide adducts of BMPO was generated in a xanthine/xanthine oxidase incubation system. BMPO superoxide adducts did not decay to the corresponding hydroxyl adducts. Results also indicate that the BMPO superoxide adduct is persistent. The decay kinetics of BMPO/•OOH also demonstrate this feature. In this system, the ESR spectrum of the BMPO/•OOH adduct could be detected even up to 35 min. Although BMPO/•OOH is intrinsically more stable, it is likely to be enzymatically reduced to BMPO/•OH in biological systems.[1]
References:
[1]. Zhao H, Joseph J, Zhang H, Karoui H, Kalyanaraman B. Synthesis and biochemical applications of a solid cyclic nitrone spin trap: a relatively superior trap for detecting superoxide anions and glutathiyl radicals. Free Radic Biol Med. 2001 Sep 1;31(5):599-606.
[2]. Duquaine D, Hirsch GA, Chakrabarti A, Han Z, Kehrer C, Brook R, Joseph J, Schott A, Kalyanaraman B, Vasquez-Vivar J, Rajagopalan S. Rapid-onset endothelial dysfunction with adriamycin: evidence for a dysfunctional nitric oxide synthase. Vasc Med. 2003 May;8(2):101-7.
This plan only provides a guide, please modify it to meet your specific needs.
BMPO detects O2− produced by hypoxanthine and xanthine oxidase
1. Prepare sodium phosphate buffer with a concentration of 50 mM and adjust the pH to 7.4;
2. Add DTPA to the sodium phosphate buffer to a final concentration of 1 mM and hypoxanthine to a final concentration of 0.5–400 μM;
3． Add xanthine oxidase (0.04 U/mL) to the sodium phosphate buffer containing DTPA and hypoxanthine to control the O2− generation rate within the range of 0.1-6.0 μM/min;
4． Add 100 mM BMPO to the buffer for spin trapping to achieve a final BMPO concentration of 50mM in the reaction mixture(Recommended working concentration 25-100mM);
5. Record the EPR spectrum 10 minutes after mixing the reagents. It is reported that the half-life of BMPO-OOH at ambient temperature is 1.23 min. The solution used for control experiments contained SOD (30 U/mL).
Precautions:
1) Fluorescent dyes all have quenching problems. Please avoid light as much as possible to slow down fluorescence quenching.
2) For your safety and health, please wear a lab coat and disposable gloves.
References:
[1]. Mitchell DG, Rosen GM, Tseitlin M, Symmes B, Eaton SS, EatonGR. Use of rapid-scan EPR to improve detection sensitivity for spin-trapped radicals. Biophys J.2013 Jul 16;105(2):338 -42.doi:10.1016/j.bpj.2013.06.005.
| Cas No. | 387334-31-8 | SDF | |
| Synonyms | BocMPO | ||
| Chemical Name | 3,4-dihydro-2-methyl-1,1-dimethylethyl ester-2H-pyrrole-2-carboxylic acid-1-oxide | ||
| Canonical SMILES | [O-][N+]1=CCCC1(C)C(OC(C)(C)C)=O | ||
| Formula | C10H17NO3 | M.Wt | 199.2 |
| Solubility | 33mg/mL in ethanol, 25mg/mL in DMSO or in DMF, 10mg/mL in water | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 5.0201 mL | 25.1004 mL | 50.2008 mL |
| 5 mM | 1.004 mL | 5.0201 mL | 10.0402 mL |
| 10 mM | 0.502 mL | 2.51 mL | 5.0201 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 18 reference(s) in Google Scholar.)
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